##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546106_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	524101
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.08650622685322	28.0	25.0	30.0	16.0	33.0
2	26.272531439550775	30.0	25.0	31.0	10.0	33.0
3	26.524784345002203	30.0	25.0	31.0	16.0	33.0
4	27.821416101095018	32.0	19.0	35.0	10.0	37.0
5	31.358034043056588	33.0	30.0	35.0	19.0	37.0
6	29.937315517428893	33.0	28.0	35.0	17.0	36.0
7	29.87835359978325	32.0	28.0	35.0	17.0	35.0
8	32.45534734717163	33.0	32.0	35.0	28.0	35.0
9	27.1874257061139	31.0	23.0	34.0	10.0	35.0
10	31.422824989839743	34.0	29.0	37.0	17.0	39.0
11	33.636966920498146	35.0	32.0	37.0	27.0	39.0
12	33.116401228007575	35.0	31.0	38.0	24.0	39.0
13	33.098956117236945	35.0	31.0	38.0	23.0	39.0
14	32.872404364807544	35.0	31.0	39.0	21.0	40.0
15	31.914386730801887	34.0	27.0	38.0	18.0	40.0
16	30.587688250928732	34.0	27.0	38.0	17.0	40.0
17	30.510586699891814	32.0	26.0	38.0	17.0	40.0
18	31.518634766962858	34.0	28.0	38.0	18.0	39.0
19	31.213914875186273	34.0	27.0	37.0	18.0	39.0
20	31.583423805716837	34.0	29.0	36.0	19.0	39.0
21	32.53127927632269	34.0	31.0	37.0	23.0	39.0
22	32.81255139753597	34.0	31.0	37.0	25.0	39.0
23	34.131058708149766	35.0	33.0	38.0	28.0	39.0
24	33.764083640367026	35.0	33.0	38.0	26.0	40.0
25	32.66154806039294	35.0	31.0	37.0	23.0	39.0
26	26.223485549541024	33.0	19.0	36.0	0.0	39.0
27	29.487327824217086	33.0	23.0	37.0	12.0	39.0
28	30.96816644120122	34.0	27.0	36.0	18.0	39.0
29	32.26559575349026	34.0	30.0	37.0	23.0	39.0
30	32.020507497600654	34.0	30.0	37.0	21.0	39.0
31	30.914394362918596	34.0	29.0	36.0	18.0	39.0
32	31.06806321682271	34.0	29.0	37.0	18.0	39.0
33	30.626720803814532	34.0	29.0	37.0	16.0	38.0
34	30.52367387202085	34.0	29.0	37.0	14.0	39.0
35	29.60410493397265	33.0	26.0	37.0	10.0	39.0
36	29.347948200823886	33.0	25.0	37.0	10.0	39.0
37	29.44905848300232	33.0	25.0	37.0	9.0	39.0
38	29.19582103449526	33.0	25.0	36.0	9.0	38.0
39	29.147475391193684	33.0	24.0	36.0	9.0	39.0
40	29.0226483063379	33.0	24.0	36.0	9.0	38.0
41	28.42599995039124	33.0	23.0	36.0	8.0	38.0
42	28.62905813955707	33.0	23.0	36.0	8.0	38.0
43	28.2074695526244	32.0	23.0	35.0	8.0	38.0
44	27.8871496142919	32.0	23.0	35.0	8.0	38.0
45	27.289356440838695	31.0	23.0	35.0	8.0	38.0
46	26.83566717102238	31.0	21.0	34.0	7.0	38.0
47	26.927368961326156	31.0	22.0	34.0	7.0	37.0
48	27.065678180350734	31.0	22.0	34.0	7.0	37.0
49	26.922938517575812	31.0	22.0	34.0	7.0	37.0
50	28.65352288967203	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	17.0
12	30.0
13	76.0
14	226.0
15	438.0
16	912.0
17	1708.0
18	2730.0
19	4420.0
20	6355.0
21	8868.0
22	11769.0
23	15356.0
24	19928.0
25	24379.0
26	27824.0
27	30227.0
28	32679.0
29	36929.0
30	43976.0
31	50832.0
32	54816.0
33	53020.0
34	44824.0
35	33045.0
36	15572.0
37	3050.0
38	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.853608369379184	22.30676911511331	26.268410096527194	27.571212418980313
2	40.23613769101757	16.510558079454153	29.565484515389212	13.687819714139069
3	28.163846281537335	13.677897962415642	44.342025678256675	13.816230077790351
4	25.94671637718684	22.794461372903317	37.44182896044846	13.816993289461383
5	17.33310945790983	28.193039127954343	40.78526848832572	13.688582925810103
6	17.12017340169166	33.58226754003522	39.24911419745431	10.048444860818812
7	75.1221615680947	3.416707848296416	19.830147242611634	1.6309833409972507
8	77.30532855308422	1.9812974980013394	18.95378944134814	1.75958450756629
9	65.49901641095896	2.758628584948321	18.814694114302398	12.927660889790326
10	43.88963196025193	19.283878489069856	26.110043674787875	10.716445875890335
11	38.391645885048874	18.44491805968697	30.123964655667514	13.039471399596641
12	33.96425498138718	17.897504488638642	35.990391165061695	12.147849364912489
13	19.01866243338593	32.06099587674895	35.68358007330648	13.23676161655864
14	13.170934609932056	35.30407306988538	38.11345523095739	13.411537089225167
15	11.192117549861573	19.690670309730375	51.788491149606664	17.32872099080139
16	13.052064392168687	13.625808765867648	52.287631582462154	21.034495259501508
17	14.755552841914058	15.120749626503288	38.66182281659451	31.46187471498814
18	18.26708974033631	20.741421977824885	43.21838729557852	17.773100986260282
19	25.251812150711412	21.996332767920688	35.18062358209582	17.571231499272084
20	26.135802068685233	19.953024321647927	34.73967803915657	19.171495570510263
21	20.093264466200218	23.086580639991148	38.584547634902435	18.235607258906203
22	18.875751047985027	19.571800091967006	35.50231730143618	26.050131558611795
23	16.457705671235125	25.990601048271227	35.51014022106426	22.041553059429383
24	16.410958956384363	21.034113653665994	47.160375576463316	15.394551813486332
25	17.42622128177584	19.190766665203846	44.120312687821624	19.262699365198692
26	12.230657831219554	38.89670120835488	30.67462187631773	18.198019084107834
27	14.326437079875825	28.25447766747249	39.735470834820006	17.683614417831677
28	13.032030085804072	23.914856105979574	46.99685747594452	16.056256332271836
29	14.350859853348878	21.590304158931197	44.990183189881336	19.068652797838585
30	16.98642055634315	24.639907193460804	41.98847168770905	16.385200562487
31	26.859326732824396	22.190379335280795	33.97971001772559	16.970583914169215
32	26.404643379806565	21.77442897456788	34.74196767416967	17.078959971455884
33	24.41075288923318	22.279102692038364	33.27373922202018	20.03640519670827
34	17.798286971404366	23.446434942883148	34.40291088931332	24.352367196399165
35	18.336160396564786	22.220144590451078	36.619086779075026	22.824608233909114
36	27.67405519165199	22.593927506339426	30.870194867019908	18.861822434988675
37	18.15451601885896	29.295879992596845	32.077404927676156	20.47219906086804
38	19.38290520338637	30.771549758538907	28.642379999274947	21.203165038799774
39	18.585539810074774	27.20906848107521	30.785096765699745	23.42029494315027
40	21.872120068460088	22.848076992793374	32.222033539336884	23.057769399409654
41	17.081058803551226	21.327759344095888	33.251033674806955	28.34014817754593
42	20.382903295357195	23.473338154287056	27.711452563532603	28.432305986823152
43	20.252012493775055	22.713751738691588	29.76773560821292	27.266500159320433
44	18.232172806386554	26.230821921728825	32.4256202525849	23.111385019299714
45	18.14497587297105	32.65114930137511	27.289205706533664	21.91466911912017
46	21.346458030036196	27.53801271129038	29.33423137906625	21.781297879607177
47	21.080097156845724	24.751145294513844	29.5815119604809	24.587245588159533
48	19.263271773951967	23.999954207299737	34.16345322752676	22.57332079122154
49	19.382141991715336	23.355612754030233	34.05736680525319	23.204878449001242
50	18.534213825197813	27.878977525324316	32.68358579739402	20.903222852083854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	506.5
2	724.0
3	6751.0
4	12778.0
5	9982.0
6	7186.0
7	7322.5
8	7459.0
9	7876.0
10	8293.0
11	8696.0
12	9099.0
13	9041.0
14	8983.0
15	8557.0
16	8131.0
17	7604.0
18	7077.0
19	6687.0
20	6297.0
21	5809.0
22	5321.0
23	5152.0
24	4983.0
25	4624.5
26	4266.0
27	4465.5
28	4665.0
29	4936.5
30	5208.0
31	6031.0
32	6854.0
33	8181.0
34	9508.0
35	10529.0
36	11550.0
37	12225.0
38	12900.0
39	16118.0
40	19336.0
41	24799.0
42	30262.0
43	36833.0
44	43404.0
45	43908.5
46	44413.0
47	43831.0
48	43249.0
49	42556.5
50	41864.0
51	38608.5
52	35353.0
53	31481.5
54	27610.0
55	25038.0
56	22466.0
57	20266.0
58	18066.0
59	16548.5
60	15031.0
61	13420.5
62	11810.0
63	10510.0
64	9210.0
65	7912.5
66	6615.0
67	5735.5
68	4856.0
69	4052.5
70	3249.0
71	2827.5
72	2406.0
73	2011.5
74	1617.0
75	1199.5
76	782.0
77	628.0
78	474.0
79	338.0
80	202.0
81	162.5
82	123.0
83	95.0
84	67.0
85	49.0
86	31.0
87	25.5
88	20.0
89	13.0
90	6.0
91	4.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	524101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.77863458875695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.37572390847508	40.313568807848746
2	6.345906975266644	6.063975410110178
3	2.326070660166163	3.3340944029912336
4	1.3127310122484943	2.5088198139019937
5	0.844264130740175	2.016889369951468
6	0.604643095840481	1.733341291966626
7	0.4598637020917389	1.5380161848011926
8	0.3250855351988456	1.2425714397084904
9	0.30442010028938504	1.3090299059879347
>10	2.821554748937032	28.532949593691182
>50	0.2523280806109082	7.706416633724167
>100	0.026102904890566745	1.859101560932131
>500	8.700968296855582E-4	0.2271911903062186
>1k	0.0	0.0
>5k	4.350484148427791E-4	1.6140343940784883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7765	1.4815846563925654	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	566	0.10799445145115158	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	527	0.10055313765858108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03911459814043476	0.0
2	0.0	0.0	0.0	0.1570308013150137	0.0
3	0.0	0.0	0.0	0.19996145781061284	0.0
4	0.0	0.0	0.0	0.26025517982220986	0.0
5	0.0	0.0	0.0	0.41823999572601467	0.0
6	0.0	0.0	0.0	0.5498940089791853	0.0
7	0.0	0.0	0.0	0.6288864169310877	0.0
8	0.0	0.0	0.0	0.8536522540502689	0.0
9	0.0	0.0	0.0	0.9872142964810218	0.0
10	0.0	0.0	0.0	1.2301064107872337	0.0
11	0.0	0.0	0.0	1.44685852536057	0.0
12	0.0	0.0	0.0	1.6353718081056896	0.0
13	0.0	0.0	0.0	1.712646989797768	0.0
14	0.0	0.0	0.0	1.7357341428465125	0.0
15	0.0	0.0	0.0	1.7687430476186843	0.0
16	0.0	0.0	0.0	1.8521239226790256	0.0
17	0.0	0.0	0.0	1.968513702511539	0.0
18	0.0	0.0	0.0	2.143289175178067	0.0
19	0.0	0.0	0.0	2.203010488436389	0.0
20	0.0	0.0	0.0	2.2682650863097	0.0
21	0.0	0.0	0.0	2.3396253775512736	0.0
22	0.0	0.0	0.0	2.4149925300657697	0.0
23	0.0	0.0	0.0	2.497991799290595	0.0
24	0.0	0.0	0.0	2.5596211417264993	0.0
25	0.0	0.0	0.0	2.6046506303174386	0.0
26	0.0	0.0	0.0	2.6525421626747514	0.0
27	0.0	0.0	0.0	2.717605957630304	0.0
28	0.0	0.0	0.0	2.789347854707394	0.0
29	0.0	0.0	0.0	2.8776896056294494	0.0
30	0.0	0.0	0.0	2.970992232413218	0.0
31	0.0	0.0	0.0	3.0558995308156254	0.0
32	0.0	0.0	0.0	3.135273544603044	0.0
33	0.0	0.0	0.0	3.2203716459232092	0.0
34	0.0	0.0	0.0	3.3033709151480344	0.0
35	0.0	0.0	0.0	3.4327352933881063	0.0
36	0.0	0.0	0.0	3.517070183037239	0.0
37	0.0	0.0	0.0	3.600069452262064	0.0
38	0.0	0.0	0.0	3.690891641114976	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	20	7.852555E-4	44.000004	30
ACACACG	20	7.852555E-4	44.000004	28
ACGTCTT	20	7.852555E-4	44.000004	31
CACCGTG	20	7.852555E-4	44.000004	18
TACGTTG	20	7.852555E-4	44.000004	1
TACCGAA	20	7.852555E-4	44.000004	18
CCGATGT	25	4.4395158E-5	44.0	11
CTATCGA	25	4.4395158E-5	44.0	38
GGCCGTA	30	2.525072E-6	44.0	8
ACGGGAC	105	0.0	44.0	5
ATCGTAG	25	4.4395158E-5	44.0	1
CGGGATA	25	4.4395158E-5	44.0	6
TAGGACG	25	4.4395158E-5	44.0	1
ACGGGTA	45	4.783942E-10	43.999996	5
CGTTTTT	8345	0.0	43.34092	1
GCTTAAA	130	0.0	42.30769	22
CGTCTTT	105	0.0	41.904762	1
GACGGTC	90	0.0	41.555553	29
CGGTCTA	90	0.0	41.555553	31
CTAGGGT	85	0.0	41.411766	4
>>END_MODULE