FastQCFastQC Report
Thu 26 May 2016
SRR1546105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546105_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences445533
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101902.287148202265601No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7200.16160419093535158No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7150.16048193960941165No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6190.13893471415136477No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4680.10504272410797853No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC4680.10504272410797853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG302.5242134E-644.0000041
TGAATAG302.5242134E-644.0000041
TAATACC254.438437E-544.010
CGGAAAG254.438437E-544.013
TAGACTT207.8512856E-444.027
GCGCGAC1800.044.09
ATCCGTA207.8512856E-444.013
TATTGCG254.438437E-544.01
TCGGATG207.8512856E-444.041
CCGTAGT207.8512856E-444.036
GTTAACG207.8512856E-444.01
CATAGCG254.438437E-544.019
CGGATAA351.4434227E-744.021
ACGCTAG207.8512856E-444.01
CGTAATG207.8512856E-444.02
CGTAAGG408.292773E-944.02
ACCGTAG207.8512856E-444.035
ACGTAGG700.044.02
TTGCACG207.8512856E-444.01
GTAAGCG207.8512856E-444.01
Produced by FastQC (version 0.11.5)