##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546105_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	445533
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.10031580152312	31.0	28.0	33.0	25.0	33.0
2	28.856836642852493	31.0	28.0	33.0	16.0	33.0
3	28.373738870072476	31.0	28.0	33.0	16.0	34.0
4	31.99466257269383	35.0	32.0	35.0	25.0	37.0
5	33.66848920281999	35.0	33.0	35.0	28.0	37.0
6	33.5137015664384	35.0	33.0	37.0	28.0	37.0
7	34.17461781731095	35.0	33.0	37.0	30.0	37.0
8	34.69725699330914	35.0	35.0	37.0	32.0	37.0
9	36.51308208370648	39.0	37.0	39.0	32.0	39.0
10	36.290158080321774	38.0	35.0	39.0	31.0	39.0
11	36.37811116123834	39.0	35.0	39.0	32.0	39.0
12	35.215357784945226	37.0	34.0	39.0	30.0	39.0
13	35.21360931737941	37.0	34.0	39.0	27.0	39.0
14	36.03030303030303	38.0	34.0	40.0	29.0	41.0
15	36.64702502396007	38.0	35.0	40.0	31.0	41.0
16	36.310062329838644	38.0	34.0	40.0	31.0	41.0
17	35.82304116642314	37.0	34.0	40.0	30.0	41.0
18	35.44558539995915	37.0	34.0	39.0	29.0	40.0
19	34.84551537147641	37.0	33.0	39.0	27.0	40.0
20	35.117744364614964	35.0	34.0	39.0	30.0	40.0
21	35.313420105805854	35.0	34.0	39.0	30.0	40.0
22	35.62981417762545	35.0	34.0	39.0	31.0	40.0
23	35.967100080128745	36.0	35.0	40.0	31.0	41.0
24	35.659286741947284	35.0	35.0	40.0	31.0	41.0
25	35.287305317451235	35.0	34.0	39.0	30.0	41.0
26	35.34020600045339	35.0	34.0	39.0	30.0	41.0
27	35.62232876128143	35.0	34.0	40.0	31.0	41.0
28	35.571569333809165	36.0	35.0	40.0	31.0	41.0
29	35.70643700915532	36.0	35.0	40.0	31.0	41.0
30	35.31571398751608	36.0	34.0	40.0	30.0	41.0
31	34.572289819160424	35.0	34.0	39.0	27.0	41.0
32	34.36292485629572	35.0	34.0	40.0	24.0	41.0
33	33.88103462594241	35.0	33.0	40.0	22.0	41.0
34	33.4201013168497	35.0	33.0	40.0	18.0	41.0
35	33.03226697012342	35.0	33.0	40.0	15.0	41.0
36	32.57186785266187	35.0	33.0	40.0	12.0	41.0
37	32.46229123319709	35.0	32.0	40.0	10.0	41.0
38	32.32504214053729	35.0	32.0	40.0	10.0	41.0
39	32.1417268754503	35.0	31.0	39.0	10.0	40.0
40	31.953516350079568	35.0	31.0	39.0	10.0	40.0
41	31.681861949619893	35.0	31.0	39.0	10.0	40.0
42	31.853945723436873	35.0	31.0	39.0	10.0	40.0
43	31.639602453690298	35.0	31.0	39.0	10.0	40.0
44	31.462428147858855	35.0	30.0	39.0	10.0	40.0
45	31.385580866063794	35.0	30.0	39.0	10.0	40.0
46	31.203192580572033	35.0	30.0	38.0	8.0	40.0
47	31.163307768448128	35.0	30.0	38.0	8.0	40.0
48	31.16350079567619	35.0	30.0	38.0	8.0	40.0
49	31.165498403036363	35.0	30.0	38.0	8.0	40.0
50	31.29867147888035	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	6.0
13	9.0
14	22.0
15	49.0
16	134.0
17	334.0
18	594.0
19	1022.0
20	1635.0
21	2432.0
22	3394.0
23	4777.0
24	7372.0
25	11135.0
26	15750.0
27	16949.0
28	15362.0
29	13981.0
30	14457.0
31	16816.0
32	22237.0
33	34120.0
34	55125.0
35	40235.0
36	39089.0
37	53975.0
38	60944.0
39	13575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.72222484080865	21.694689282275387	25.777439606044894	26.80564627087107
2	41.25575434367376	19.78169967207816	27.14658622369162	11.815959760556458
3	26.252600817447863	17.499713825911886	43.531904482945144	12.7157808736951
4	24.020891830683695	22.694615213687875	40.094224221325916	13.19026873430251
5	18.303021325019696	27.560472512698276	40.562876375038435	13.573629787243593
6	17.17111863767667	34.786873250690746	38.08068089232447	9.96132721930811
7	72.13158172346381	4.313709646647947	21.158028698210906	2.396679931677339
8	73.04307425039222	3.3952591614986996	20.749529215568767	2.8121373725403056
9	69.75465341512302	5.008158767139583	21.530840588688154	3.706347229049251
10	39.59437348075227	25.390487348860802	25.754545678995722	9.26059349139121
11	36.17083358583989	17.632812833168362	33.88278758251353	12.313565998478229
12	32.808793063589	18.63161651325491	36.507733433887054	12.051856989269034
13	18.060839488881854	33.185420608574454	37.124298312358455	11.629441590185237
14	13.531208687123064	36.100131752305664	37.21295616710771	13.155703393463558
15	11.583429285821701	22.161321383601216	53.078447612185855	13.176801718391228
16	14.26134539977959	16.52627302579158	52.575679018164756	16.636702556264073
17	14.165729586809505	17.127350835965014	38.75762289213145	29.949296685094033
18	17.638872990328437	21.496275247849205	42.94317143735705	17.921680324465303
19	22.658029820462232	22.342901648138298	36.845531082994974	18.153537448404496
20	25.700004264555037	20.422056278659493	35.78141237573872	18.096527081046744
21	20.278632559204368	24.447571784806062	38.07866083993778	17.195134816051784
22	18.907914789701323	20.33182727205392	36.084420233742506	24.67583770450225
23	16.13887186807711	26.746615851126627	36.16881353345319	20.94569874734307
24	16.64904732084941	22.03136468005737	46.37232258889914	14.947265410194083
25	16.57722323598925	21.68548682140268	43.84187029916976	17.89541964343831
26	14.76321619273993	28.34537509006067	37.70382889707384	19.18757982012556
27	14.698574516365792	29.20008169989653	39.95169830293154	16.149645480806136
28	13.184881927937997	24.24938220064507	47.08248322795394	15.483252643462997
29	13.431328319114408	21.926770856479767	45.08038686247708	19.561513961928746
30	16.146278726828317	25.628853530490446	42.24804896606985	15.976818776611385
31	25.191399963639054	23.225664541122658	34.894160477450605	16.688775017787684
32	24.894676713060537	23.538997111325084	34.89640498010249	16.66992119551189
33	23.052613386662717	23.398491245317405	34.06571454864174	19.483180819378138
34	17.28738387504405	24.699629432612173	34.53773345633208	23.4752532360117
35	17.76815634307672	23.047002130033018	36.90074584823122	22.284095678659043
36	27.019996274125596	23.22521564059228	32.43014546621687	17.32464261906525
37	18.32928200604669	28.356822053585258	33.82196156064759	19.49193437972047
38	18.700971645198	30.624218632514317	30.194620824944508	20.480188897343183
39	18.90903704102726	27.826446076946038	31.76711938285155	21.497397499175143
40	21.155784195559026	24.51176456064983	32.70150583682915	21.630945406962
41	16.41337454240202	23.196934907178594	34.17143062354528	26.218259926874104
42	20.183016746234287	24.96223624288212	29.42206301216745	25.432683998716143
43	20.005476586470586	23.787239104623	30.64823481088943	25.559049498016982
44	18.015500535313883	26.694318939337826	32.94503437455811	22.34514615079018
45	17.873423517449886	32.14329802730662	28.144267652452232	21.839010802791265
46	21.686833522993805	28.89729829215793	29.494784898088355	19.921083286759902
47	20.514305337651756	25.393180752043058	30.66282407812665	23.42968983217854
48	19.713242341195826	24.69671157916473	33.622874175425835	21.967171904213608
49	19.061663221355097	24.312228274897706	34.835130057706166	21.790978446041034
50	18.40222834223279	27.79524749008491	32.68848772144825	21.11403644623406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	382.5
2	536.0
3	7496.5
4	14457.0
5	10601.5
6	6746.0
7	6872.5
8	6999.0
9	7374.0
10	7749.0
11	7809.0
12	7869.0
13	7564.5
14	7260.0
15	6872.5
16	6485.0
17	6004.5
18	5524.0
19	5003.0
20	4482.0
21	4374.5
22	4267.0
23	3762.0
24	3257.0
25	3336.0
26	3415.0
27	3730.5
28	4046.0
29	4299.0
30	4552.0
31	5462.5
32	6373.0
33	7695.0
34	9017.0
35	9845.0
36	10673.0
37	11968.5
38	13264.0
39	16105.0
40	18946.0
41	25555.5
42	32165.0
43	35832.5
44	39500.0
45	41932.0
46	44364.0
47	42526.0
48	40688.0
49	39213.5
50	37739.0
51	32714.0
52	27689.0
53	24037.5
54	20386.0
55	17906.0
56	15426.0
57	13941.0
58	12456.0
59	10430.5
60	8405.0
61	7512.0
62	6619.0
63	5535.5
64	4452.0
65	3750.0
66	3048.0
67	2595.0
68	2142.0
69	1875.0
70	1608.0
71	1389.0
72	1170.0
73	1002.5
74	835.0
75	583.5
76	332.0
77	242.5
78	153.0
79	147.5
80	142.0
81	87.5
82	33.0
83	21.5
84	10.0
85	9.5
86	9.0
87	7.5
88	6.0
89	5.5
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	445533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.480002986017006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61948493071485	27.89419560100882
2	7.7355690722572366	5.489160275644552
3	3.3544327745854243	3.570458545760525
4	2.0056541546727575	2.8464246158682744
5	1.2735367350744173	2.2592543581621336
6	0.8639316587807896	1.8391378699954224
7	0.6239538960566101	1.5496520266657827
8	0.49659305779158214	1.4095298538624519
9	0.3821219784996848	1.2201920044372387
>10	3.9607660469968087	31.271889294235617
>50	0.626959386689025	14.840915214413124
>100	0.054463138641672876	3.0580598950516675
>500	0.0018998769293606813	0.4615176358880327
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.332923097868939E-4	2.289612809006355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10190	2.287148202265601	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	720	0.16160419093535158	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	715	0.16048193960941165	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	619	0.13893471415136477	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	468	0.10504272410797853	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	468	0.10504272410797853	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05970377054000489	0.0
2	0.0	0.0	0.0	0.24375298799415532	0.0
3	0.0	0.0	0.0	0.32724848664408696	0.0
4	0.0	0.0	0.0	0.49738178765658214	0.0
5	0.0	0.0	0.0	0.922266139657444	0.0
6	0.0	0.0	0.0	1.212929233075889	0.0
7	0.0	0.0	0.0	1.4086498643198146	0.0
8	0.0	0.0	0.0	1.84318557772376	0.0
9	2.2445026518798833E-4	0.0	0.0	1.979651338958057	0.0
10	2.2445026518798833E-4	0.0	0.0	2.3342827579550787	0.0
11	2.2445026518798833E-4	0.0	0.0	2.638412867284803	0.0
12	2.2445026518798833E-4	0.0	0.0	2.9528676888131744	0.0
13	2.2445026518798833E-4	0.0	0.0	3.0639705700812288	0.0
14	4.4890053037597666E-4	0.0	0.0	3.1012293141024347	0.0
15	4.4890053037597666E-4	0.0	0.0	3.1775424042663505	0.0
16	4.4890053037597666E-4	0.0	0.0	3.324557327964483	0.0
17	4.4890053037597666E-4	0.0	0.0	3.514891152843897	0.0
18	4.4890053037597666E-4	0.0	0.0	3.80241194254971	0.0
19	4.4890053037597666E-4	0.0	0.0	3.9184527296519	0.0
20	6.733507955639649E-4	0.0	0.0	4.0412270247097295	0.0
21	6.733507955639649E-4	0.0	0.0	4.2061979696229015	0.0
22	6.733507955639649E-4	0.0	0.0	4.383962579651788	0.0
23	6.733507955639649E-4	0.0	0.0	4.57788760877421	0.0
24	6.733507955639649E-4	0.0	0.0	4.713455568947754	0.0
25	6.733507955639649E-4	0.0	0.0	4.845432324878292	0.0
26	6.733507955639649E-4	0.0	0.0	4.9605753109197295	0.0
27	6.733507955639649E-4	0.0	0.0	5.087614161016131	0.0
28	6.733507955639649E-4	0.0	0.0	5.218693115885916	0.0
29	6.733507955639649E-4	0.0	0.0	5.352465473937958	0.0
30	6.733507955639649E-4	0.0	0.0	5.517660869116317	0.0
31	6.733507955639649E-4	0.0	0.0	5.691160924106632	0.0
32	6.733507955639649E-4	0.0	0.0	5.8366046959484486	0.0
33	6.733507955639649E-4	0.0	0.0	5.992822080519288	0.0
34	6.733507955639649E-4	0.0	0.0	6.14095925554336	0.0
35	6.733507955639649E-4	0.0	0.0	6.367429573118041	0.0
36	6.733507955639649E-4	0.0	0.0	6.5157911984073005	0.0
37	6.733507955639649E-4	0.0	0.0	6.671335232182577	0.0
38	6.733507955639649E-4	0.0	0.0	6.821941360123717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.5242134E-6	44.000004	1
TGAATAG	30	2.5242134E-6	44.000004	1
TAATACC	25	4.438437E-5	44.0	10
CGGAAAG	25	4.438437E-5	44.0	13
TAGACTT	20	7.8512856E-4	44.0	27
GCGCGAC	180	0.0	44.0	9
ATCCGTA	20	7.8512856E-4	44.0	13
TATTGCG	25	4.438437E-5	44.0	1
TCGGATG	20	7.8512856E-4	44.0	41
CCGTAGT	20	7.8512856E-4	44.0	36
GTTAACG	20	7.8512856E-4	44.0	1
CATAGCG	25	4.438437E-5	44.0	19
CGGATAA	35	1.4434227E-7	44.0	21
ACGCTAG	20	7.8512856E-4	44.0	1
CGTAATG	20	7.8512856E-4	44.0	2
CGTAAGG	40	8.292773E-9	44.0	2
ACCGTAG	20	7.8512856E-4	44.0	35
ACGTAGG	70	0.0	44.0	2
TTGCACG	20	7.8512856E-4	44.0	1
GTAAGCG	20	7.8512856E-4	44.0	1
>>END_MODULE