FastQCFastQC Report
Thu 26 May 2016
SRR1546102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546102_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences379000
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53051.3997361477572559No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT4800.1266490765171504No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT4300.11345646437994723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG254.437177E-544.01
TCGTTAG254.437177E-544.01
GTCGAGG254.437177E-544.02
TTGTGCG207.8497967E-444.01
CACGGCG207.8497967E-444.04
TCCGATG254.437177E-544.01
TGGCGTA207.8497967E-444.06
GGCGTAA207.8497967E-444.07
ATTGTCA502.7284841E-1144.010
TAACGGG502.7284841E-1144.03
AGTATCG254.437177E-544.01
GTGGACC207.8497967E-444.031
AACGGGC254.437177E-544.04
AACGGCG254.437177E-544.04
TAATTCG207.8497967E-444.025
GTAGACG302.5232093E-644.01
TTAAGCG207.8497967E-444.01
TACTGGC302.5232093E-644.03
CTTGCAT207.8497967E-444.021
TGTTCGG454.783942E-1044.02
Produced by FastQC (version 0.11.5)