##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546102_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	379000
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.14601055408971	28.0	25.0	30.0	16.0	33.0
2	26.271728232189975	30.0	25.0	31.0	10.0	33.0
3	26.64305277044855	30.0	25.0	31.0	16.0	33.0
4	27.493728232189973	32.0	19.0	35.0	10.0	35.0
5	31.27964907651715	33.0	30.0	35.0	19.0	35.0
6	29.96657519788918	33.0	28.0	35.0	17.0	36.0
7	30.059261213720315	32.0	28.0	35.0	17.0	35.0
8	32.56237994722955	35.0	32.0	35.0	28.0	35.0
9	27.25626649076517	31.0	23.0	34.0	10.0	37.0
10	31.954211081794195	35.0	31.0	37.0	18.0	39.0
11	33.883736147757254	35.0	32.0	39.0	27.0	39.0
12	33.346828496042214	35.0	31.0	39.0	25.0	39.0
13	33.15427176781002	35.0	31.0	39.0	22.0	39.0
14	32.98727440633245	35.0	31.0	39.0	21.0	40.0
15	31.994883905013193	34.0	27.0	38.0	18.0	40.0
16	30.701799472295516	34.0	27.0	38.0	17.0	40.0
17	30.699696569920846	33.0	27.0	38.0	17.0	40.0
18	31.685849604221637	34.0	29.0	38.0	18.0	39.0
19	31.317506596306067	34.0	28.0	37.0	18.0	39.0
20	31.724100263852243	34.0	30.0	36.0	19.0	39.0
21	32.57502638522428	34.0	31.0	37.0	23.0	39.0
22	32.851358839050135	34.0	31.0	37.0	25.0	39.0
23	34.11921635883905	35.0	33.0	38.0	28.0	39.0
24	33.76059102902374	35.0	33.0	38.0	26.0	40.0
25	32.616591029023745	35.0	31.0	37.0	23.0	39.0
26	26.07568073878628	33.0	18.0	35.0	0.0	38.0
27	29.394158311345645	33.0	23.0	36.0	12.0	39.0
28	30.920746701846966	34.0	27.0	36.0	18.0	39.0
29	32.216416886543534	34.0	30.0	37.0	23.0	39.0
30	31.99073878627968	34.0	30.0	37.0	21.0	39.0
31	30.878139841688654	33.0	29.0	36.0	18.0	38.0
32	31.00445646437995	34.0	29.0	37.0	18.0	39.0
33	30.56777308707124	34.0	29.0	36.0	15.0	38.0
34	30.410585751978893	34.0	29.0	37.0	12.0	39.0
35	29.460765171503958	33.0	26.0	37.0	9.0	39.0
36	29.10583377308707	33.0	24.0	36.0	8.0	39.0
37	29.168498680738786	33.0	24.0	37.0	8.0	39.0
38	28.93321899736148	33.0	24.0	36.0	8.0	38.0
39	28.851121372031663	33.0	24.0	36.0	8.0	39.0
40	28.737889182058048	33.0	23.0	36.0	8.0	38.0
41	28.173788918205805	33.0	23.0	35.0	8.0	38.0
42	28.399335092348284	33.0	23.0	36.0	8.0	38.0
43	27.992435356200527	32.0	23.0	35.0	7.0	38.0
44	27.716622691292876	32.0	23.0	35.0	7.0	38.0
45	27.109862796833774	31.0	22.0	35.0	7.0	38.0
46	26.67620844327177	31.0	20.0	35.0	7.0	38.0
47	26.79380474934037	31.0	21.0	34.0	7.0	37.0
48	26.8521635883905	31.0	21.0	34.0	7.0	37.0
49	26.70608179419525	31.0	20.0	34.0	7.0	37.0
50	28.467981530343007	33.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	21.0
13	55.0
14	135.0
15	291.0
16	631.0
17	1194.0
18	2005.0
19	3224.0
20	4706.0
21	6619.0
22	8862.0
23	11669.0
24	14801.0
25	18278.0
26	20777.0
27	21727.0
28	23051.0
29	26208.0
30	31281.0
31	36205.0
32	38848.0
33	38328.0
34	32078.0
35	23962.0
36	11714.0
37	2262.0
38	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.910817941952505	22.101583113456464	25.236675461741427	28.750923482849604
2	41.76965699208443	16.839313984168864	28.76490765171504	12.626121372031662
3	27.502902374670185	13.345646437994723	45.81978891820581	13.331662269129287
4	25.048548812664905	22.439050131926123	39.57915567282322	12.933245382585751
5	17.28126649076517	27.46622691292876	42.12691292875989	13.125593667546173
6	16.83456464379947	33.522163588390505	39.70369393139841	9.939577836411608
7	74.57730870712402	2.5179419525065962	21.574934036939315	1.3298153034300793
8	75.97493403693932	1.8551451187335093	20.693139841688655	1.4767810026385224
9	64.52928759894459	2.9013192612137204	19.948284960422164	12.621108179419524
10	40.807651715039576	25.048548812664905	26.15831134564644	7.985488126649076
11	37.832189973614774	16.362269129287597	34.5042216358839	11.30131926121372
12	33.799208443271766	18.06147757255937	37.183905013192614	10.955408970976253
13	17.41292875989446	34.15488126649077	37.64353562005277	10.788654353562006
14	12.450131926121372	36.74221635883905	38.36015831134565	12.447493403693931
15	9.979419525065964	20.217678100263853	53.91662269129288	15.886279683377309
16	12.407387862796835	14.260158311345647	53.472031662269124	19.86042216358839
17	13.20976253298153	15.827176781002638	39.931398416886545	31.03166226912929
18	16.988654353562005	20.702902374670185	44.789182058047494	17.519261213720316
19	23.328232189973615	21.24485488126649	38.12585751978892	17.301055408970974
20	26.11029023746702	19.529023746701846	36.901055408970976	17.45963060686016
21	19.900527704485487	24.026912928759895	39.135092348284964	16.937467018469658
22	18.29868073878628	19.576517150395777	36.855672823218995	25.269129287598947
23	15.326912928759896	26.864643799472294	37.20738786279683	20.601055408970975
24	15.492084432717679	21.245118733509237	49.24063324538259	14.022163588390502
25	15.839050131926122	20.035620052770447	46.47730870712401	17.64802110817942
26	11.332717678100265	40.58179419525066	31.604221635883906	16.48126649076517
27	13.46939313984169	30.18232189973615	41.10343007915567	15.24485488126649
28	12.062532981530342	23.920316622691292	49.258047493403694	14.759102902374671
29	12.340633245382586	21.245118733509237	47.22374670184696	19.190501319261212
30	15.194986807387863	25.652242744063326	43.68918205804749	15.46358839050132
31	26.032189973614777	22.461741424802113	35.747229551451184	15.758839050131925
32	25.427440633245386	22.591820580474934	35.76411609498681	16.216622691292876
33	23.61873350923483	22.62532981530343	34.61767810026385	19.138258575197888
34	16.49947229551451	23.866490765171505	35.11372031662269	24.520316622691293
35	17.491292875989444	22.22717678100264	37.60184696569921	22.679683377308706
36	27.554881266490767	22.469920844327177	32.497097625329815	17.47810026385224
37	17.511081794195253	28.833245382585755	33.518997361477574	20.136675461741422
38	17.866754617414248	31.60738786279683	30.17044854881267	20.355408970976253
39	18.19868073878628	27.96807387862797	31.77229551451187	22.06094986807388
40	21.078891820580477	23.72981530343008	32.92084432717678	22.270448548812666
41	15.630870712401054	22.16253298153034	34.61372031662269	27.592875989445908
42	19.253562005277043	24.05725593667546	28.735356200527708	27.95382585751979
43	19.261213720316622	22.893667546174143	30.693403693931398	27.151715039577834
44	17.092084432717677	26.144591029023744	33.48416886543536	23.27915567282322
45	17.23509234828496	33.27598944591029	27.43298153034301	22.05593667546174
46	20.603693931398418	28.740105540897098	29.293403693931396	21.362796833773086
47	19.95514511873351	24.56807387862797	30.030343007915565	25.446437994722952
48	18.788918205804748	24.16965699208443	34.51715039577836	22.524274406332452
49	18.58918205804749	24.216622691292876	34.46965699208444	22.724538258575198
50	17.68891820580475	28.503166226912928	32.57097625329815	21.23693931398417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	226.5
2	332.0
3	4455.0
4	8578.0
5	7058.0
6	5538.0
7	5655.5
8	5773.0
9	6249.0
10	6725.0
11	7044.0
12	7363.0
13	7308.5
14	7254.0
15	7045.5
16	6837.0
17	6489.0
18	6141.0
19	5717.0
20	5293.0
21	5101.0
22	4909.0
23	4421.5
24	3934.0
25	3812.5
26	3691.0
27	3849.5
28	4008.0
29	4233.0
30	4458.0
31	5218.5
32	5979.0
33	6881.0
34	7783.0
35	8234.5
36	8686.0
37	9647.5
38	10609.0
39	12921.0
40	15233.0
41	20523.5
42	25814.0
43	29086.0
44	32358.0
45	34174.5
46	35991.0
47	34791.5
48	33592.0
49	32020.5
50	30449.0
51	26950.0
52	23451.0
53	20409.5
54	17368.0
55	15488.0
56	13608.0
57	12241.0
58	10874.0
59	9133.0
60	7392.0
61	6661.5
62	5931.0
63	4997.5
64	4064.0
65	3447.0
66	2830.0
67	2373.5
68	1917.0
69	1707.0
70	1497.0
71	1320.5
72	1144.0
73	959.5
74	775.0
75	555.5
76	336.0
77	253.5
78	171.0
79	142.5
80	114.0
81	79.5
82	45.0
83	27.0
84	9.0
85	10.0
86	11.0
87	8.5
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	379000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.08362470283373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.44539244924876	43.64860360463767
2	5.920580091073247	6.048893827909098
3	2.258720601211367	3.4615090650252136
4	1.2089806452310508	2.4703645421589107
5	0.7862254633172565	2.0081623249955154
6	0.561227730850361	1.7201728065349697
7	0.4524502275769348	1.6178958335576297
8	0.3729295415541402	1.5240474193081228
9	0.27331233217648204	1.2565606143203674
>10	2.483069858600411	26.261956258441543
>50	0.21702635767814654	6.907825642302566
>100	0.019526793107288252	1.5620840145829014
>500	0.0	0.0
>1k	0.0	0.0
>5k	5.5790837449395E-4	1.5119240462255596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5305	1.3997361477572559	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	480	0.1266490765171504	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	430	0.11345646437994723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03852242744063324	0.0
2	0.0	0.0	0.0	0.1604221635883905	0.0
3	0.0	0.0	0.0	0.21556728232189973	0.0
4	0.0	0.0	0.0	0.30395778364116094	0.0
5	0.0	0.0	0.0	0.5551451187335092	0.0
6	0.0	0.0	0.0	0.7108179419525066	0.0
7	0.0	0.0	0.0	0.8065963060686016	0.0
8	0.0	0.0	0.0	1.045382585751979	0.0
9	0.0	0.0	0.0	1.1488126649076518	0.0
10	0.0	0.0	0.0	1.3522427440633245	0.0
11	0.0	0.0	0.0	1.5630606860158311	0.0
12	0.0	0.0	0.0	1.7593667546174143	0.0
13	0.0	0.0	0.0	1.8461741424802112	0.0
14	0.0	0.0	0.0	1.8728232189973615	0.0
15	0.0	0.0	0.0	1.9105540897097626	0.0
16	0.0	0.0	0.0	1.99155672823219	0.0
17	0.0	0.0	0.0	2.1387862796833774	0.0
18	0.0	0.0	0.0	2.310817941952507	0.0
19	0.0	0.0	0.0	2.3844327176781004	0.0
20	0.0	0.0	0.0	2.4678100263852243	0.0
21	0.0	0.0	0.0	2.549868073878628	0.0
22	0.0	0.0	0.0	2.6485488126649077	0.0
23	0.0	0.0	0.0	2.758311345646438	0.0
24	0.0	0.0	0.0	2.8319261213720317	0.0
25	0.0	0.0	0.0	2.9108179419525064	0.0
26	0.0	0.0	0.0	2.978627968337731	0.0
27	0.0	0.0	0.0	3.062532981530343	0.0
28	0.0	0.0	0.0	3.1662269129287597	0.0
29	0.0	0.0	0.0	3.2651715039577835	0.0
30	0.0	0.0	0.0	3.387335092348285	0.0
31	0.0	0.0	0.0	3.504221635883905	0.0
32	0.0	0.0	0.0	3.6145118733509234	0.0
33	0.0	0.0	0.0	3.729815303430079	0.0
34	0.0	0.0	0.0	3.8403693931398415	0.0
35	0.0	0.0	0.0	4.0168865435356205	0.0
36	0.0	0.0	0.0	4.1345646437994725	0.0
37	0.0	0.0	0.0	4.251715039577836	0.0
38	0.0	0.0	0.0	4.3672823218997365	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCCG	25	4.437177E-5	44.0	1
TCGTTAG	25	4.437177E-5	44.0	1
GTCGAGG	25	4.437177E-5	44.0	2
TTGTGCG	20	7.8497967E-4	44.0	1
CACGGCG	20	7.8497967E-4	44.0	4
TCCGATG	25	4.437177E-5	44.0	1
TGGCGTA	20	7.8497967E-4	44.0	6
GGCGTAA	20	7.8497967E-4	44.0	7
ATTGTCA	50	2.7284841E-11	44.0	10
TAACGGG	50	2.7284841E-11	44.0	3
AGTATCG	25	4.437177E-5	44.0	1
GTGGACC	20	7.8497967E-4	44.0	31
AACGGGC	25	4.437177E-5	44.0	4
AACGGCG	25	4.437177E-5	44.0	4
TAATTCG	20	7.8497967E-4	44.0	25
GTAGACG	30	2.5232093E-6	44.0	1
TTAAGCG	20	7.8497967E-4	44.0	1
TACTGGC	30	2.5232093E-6	44.0	3
CTTGCAT	20	7.8497967E-4	44.0	21
TGTTCGG	45	4.783942E-10	44.0	2
>>END_MODULE