SRR1546101_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546101_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1059134
Sequences flagged as poor quality	0
Sequence length	50
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38160	3.6029435368895717	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	6599	0.6230561949668314	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4405	0.4159058249475515	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	3035	0.2865548646346921	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2088	0.19714219352791998	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2078	0.19619802593439545	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1863	0.17589842267361827	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1713	0.16173590877075045	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1649	0.15569323617219352	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1065	0.1005538487103615	TruSeq Adapter, Index 14 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	25	4.442603E-5	44.0	1
ATAGCGC	20	7.856201E-4	44.0	10
CTAACGG	60	0.0	44.0	2
ATATCCG	20	7.856201E-4	44.0	40
TATCGCG	20	7.856201E-4	44.0	32
TAATGCG	20	7.856201E-4	44.0	1
CGTTTTT	22120	0.0	43.353527	1
GTTTTTT	24455	0.0	39.66387	2
TCACGAC	195	0.0	39.48718	25
CGGTCTA	195	0.0	39.48718	31
TACGGGA	230	0.0	39.217392	4
CGACGGT	200	0.0	38.5	28
TGCGACG	80	0.0	38.5	1
TATAGCG	40	4.124122E-7	38.5	1
GGGCGAT	915	0.0	37.748634	7
TAGGGTA	565	0.0	37.38053	5
ATAGGGA	990	0.0	37.333332	4
ACAACGA	65	1.0913936E-11	37.23077	13
TAACGGG	190	0.0	37.05263	3
CGTTCGA	95	0.0	37.05263	14