##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546101_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1059134
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.13940162434593	31.0	28.0	31.0	25.0	33.0
2	29.071791671308823	31.0	28.0	33.0	23.0	33.0
3	28.47578304539369	31.0	28.0	33.0	16.0	34.0
4	32.02659720110959	35.0	32.0	35.0	25.0	37.0
5	33.715256048809685	35.0	33.0	35.0	30.0	37.0
6	33.68177208927293	35.0	33.0	37.0	28.0	37.0
7	33.99450683294087	35.0	33.0	37.0	30.0	37.0
8	34.5344687263368	35.0	35.0	37.0	32.0	37.0
9	36.290160640674365	39.0	35.0	39.0	31.0	39.0
10	36.07215423166474	37.0	35.0	39.0	31.0	39.0
11	36.24640602605525	38.0	35.0	39.0	31.0	39.0
12	35.45895703470949	37.0	34.0	39.0	29.0	39.0
13	35.96222385458309	38.0	35.0	39.0	30.0	39.0
14	36.93017219728571	39.0	35.0	41.0	31.0	41.0
15	37.15638247851547	39.0	36.0	41.0	32.0	41.0
16	36.801996725626786	38.0	35.0	41.0	31.0	41.0
17	36.343845065874575	38.0	34.0	40.0	30.0	41.0
18	35.82660834228719	38.0	34.0	39.0	29.0	40.0
19	35.156655342949996	37.0	34.0	39.0	27.0	40.0
20	35.24734736114599	35.0	34.0	39.0	29.0	40.0
21	35.589206842571386	36.0	34.0	39.0	30.0	40.0
22	35.908851004688735	36.0	35.0	40.0	31.0	41.0
23	36.15834068210444	37.0	35.0	40.0	31.0	41.0
24	35.874266145737934	36.0	35.0	40.0	31.0	41.0
25	35.60764360317014	36.0	35.0	40.0	30.0	41.0
26	35.56458295173226	36.0	34.0	40.0	30.0	41.0
27	35.870170346717224	37.0	35.0	40.0	31.0	41.0
28	35.73683216665691	37.0	35.0	40.0	31.0	41.0
29	35.7267154108923	37.0	35.0	40.0	30.0	41.0
30	35.28280935179118	36.0	34.0	40.0	29.0	41.0
31	34.554695628692876	36.0	34.0	40.0	25.0	41.0
32	34.29907547109242	36.0	34.0	40.0	23.0	41.0
33	33.69666822139597	36.0	33.0	40.0	18.0	41.0
34	33.051697896583434	36.0	33.0	40.0	15.0	41.0
35	32.58110021961338	36.0	32.0	40.0	10.0	41.0
36	32.177445913359406	36.0	31.0	40.0	10.0	41.0
37	32.056481993779826	36.0	31.0	40.0	10.0	41.0
38	31.826014460870862	36.0	30.0	40.0	9.0	41.0
39	31.479135784518295	35.0	30.0	40.0	8.0	40.0
40	31.228268566583644	35.0	29.0	40.0	8.0	40.0
41	30.8440886611137	35.0	27.0	39.0	8.0	40.0
42	31.013175858767635	35.0	28.0	39.0	8.0	40.0
43	30.86797515706228	35.0	27.0	39.0	8.0	40.0
44	30.744138135495604	35.0	27.0	39.0	8.0	40.0
45	30.682425453247653	35.0	27.0	39.0	8.0	40.0
46	30.370136356683858	35.0	26.0	39.0	7.0	40.0
47	30.311793408577195	35.0	26.0	38.0	7.0	40.0
48	30.29012192980303	35.0	26.0	38.0	7.0	40.0
49	30.189278221641455	35.0	26.0	38.0	7.0	40.0
50	30.35812560072663	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	5.0
12	15.0
13	26.0
14	53.0
15	139.0
16	327.0
17	875.0
18	1775.0
19	2960.0
20	4537.0
21	6363.0
22	9255.0
23	13166.0
24	19681.0
25	29838.0
26	41220.0
27	45745.0
28	41086.0
29	36736.0
30	36735.0
31	40713.0
32	49076.0
33	65341.0
34	92832.0
35	93545.0
36	101808.0
37	138144.0
38	153652.0
39	33478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.121969458066687	19.30530036803653	21.90978667477392	31.66294349912287
2	46.09813300300056	20.38929918216203	22.54219012891664	10.970377685920761
3	25.11136456765622	19.50064864313675	43.71883066731877	11.669156121888259
4	22.70118795166617	20.5805875366101	43.77897414302628	12.939250368697444
5	19.15895439104023	24.559687442759838	42.08891415061739	14.192444015582542
6	16.52312172019782	33.60018656751648	40.65217432355113	9.22451738873457
7	64.70644885349729	4.650308648386323	27.800448290773407	2.84279420734298
8	64.73666221649007	5.3305813995207405	26.458502890097	3.47425349389218
9	61.231251192011584	5.452756686122814	26.82399016555034	6.492001956315254
10	29.108025991045515	27.12366895973503	32.61541976747041	11.152885281749052
11	24.60746232299218	19.99492037834684	40.17895752567664	15.218659772984344
12	22.570798407000435	17.766023940313502	44.66677493121739	14.996402721468671
13	18.82802364950988	21.820940504223262	45.13970847881382	14.211327367453034
14	15.28210783526919	27.30249430194857	42.69289816019503	14.722499702587207
15	14.65357546825992	21.996272426340767	48.43126554335901	14.91888656204031
16	17.2904467234552	19.017046001733494	46.77821692061628	16.914290354195032
17	16.279432064309145	19.269044332445187	42.30928286694601	22.142240736299655
18	17.422724603307984	22.074732753362653	43.33710370925681	17.165438934072554
19	20.090942222608284	22.95979545553254	39.55136932626089	17.39789299559829
20	20.42234504793539	22.05027881269037	40.23674058240034	17.290635556973903
21	20.148914112850687	22.30605381377616	42.19843759146624	15.346594481906916
22	17.75695993141567	19.14752996315858	41.39863322299161	21.69687688243414
23	16.82147867975157	23.479465299008435	41.6174912711706	18.081564750069397
24	17.86884379124832	22.726208392894574	43.39044917829094	16.014498637566163
25	19.765393236361025	22.054338733342522	41.00038333204297	17.179884698253478
26	15.845209388047216	22.703265120371928	40.5452001352048	20.90632535637606
27	16.977927249998583	22.056321485288922	42.99361553873259	17.972135725979903
28	15.482743448893151	22.35618911299231	45.109306282302335	17.0517611558122
29	17.32981851210517	22.834032332075072	42.93403856358119	16.902110592238564
30	17.438775452397902	21.91922835070916	45.06899032605884	15.573005870834097
31	21.935373616558433	21.26529787543408	39.97180715565736	16.82752135235013
32	22.05528290093605	22.91372007696854	38.69274331670969	16.33825370538572
33	20.390715433552316	21.04568449318028	37.23098304841503	21.33261702485238
34	18.730774387376854	23.706254354973026	37.308876874880795	20.25409438276932
35	18.798093536795154	22.6425551441083	38.137572771717274	20.421778547379272
36	22.614985450377382	24.222619611871586	34.01798072765108	19.14441421009995
37	19.070674721045684	25.428227212043048	34.99746018917342	20.503637877737848
38	19.699868005370426	27.39719431157908	32.462464617319434	20.44047306573106
39	20.72089084100784	24.24858422069351	32.31611864032313	22.714406297975515
40	20.01002705984323	23.35266359119809	36.324865408909545	20.312443940049135
41	18.34442100810662	23.661217560761905	35.47350948982848	22.52085194130299
42	19.914949383175312	25.677393039974167	33.22100886195704	21.18664871489349
43	20.158639039063992	23.49617706541382	32.81841580007818	23.526768095444016
44	19.594782152211145	24.115645423525258	34.59222345803269	21.6973489662309
45	20.762434215122923	25.087005043743282	32.3169683911573	21.83359234997649
46	22.482801987283953	24.60019223252204	32.251065493129296	20.665940287064714
47	20.493724118005844	24.698196828729888	33.89023485224721	20.917844201017058
48	19.13629436879564	26.133425987646515	33.05351353086578	21.676766112692068
49	18.951709604261595	24.69017140418493	35.05363816098813	21.304480830565346
50	19.077661561237765	23.655741388719463	36.19768603406179	21.06891101598098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2114.0
1	2199.5
2	2285.0
3	30576.5
4	58868.0
5	40298.0
6	21728.0
7	21162.5
8	20597.0
9	20928.0
10	21259.0
11	20908.0
12	20557.0
13	19762.5
14	18968.0
15	17839.5
16	16711.0
17	15726.5
18	14742.0
19	13455.5
20	12169.0
21	11350.5
22	10532.0
23	10359.0
24	10186.0
25	9959.5
26	9733.0
27	10598.5
28	11464.0
29	12847.0
30	14230.0
31	15688.0
32	17146.0
33	19360.5
34	21575.0
35	23550.5
36	25526.0
37	27710.0
38	29894.0
39	34176.0
40	38458.0
41	44556.5
42	50655.0
43	55518.0
44	60381.0
45	72257.0
46	84133.0
47	83439.5
48	82746.0
49	82338.5
50	81931.0
51	73488.0
52	65045.0
53	55667.5
54	46290.0
55	42372.0
56	38454.0
57	36644.0
58	34834.0
59	32655.5
60	30477.0
61	27425.5
62	24374.0
63	22048.0
64	19722.0
65	16515.0
66	13308.0
67	11129.5
68	8951.0
69	7824.0
70	6697.0
71	5968.0
72	5239.0
73	4328.5
74	3418.0
75	2598.5
76	1779.0
77	1330.0
78	881.0
79	689.0
80	497.0
81	370.5
82	244.0
83	200.0
84	156.0
85	119.5
86	83.0
87	63.5
88	44.0
89	31.5
90	19.0
91	16.5
92	14.0
93	13.5
94	13.0
95	7.5
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1059134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.94912424876714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87930505581474	28.77572118914938
2	7.344046465389428	5.4271217067678625
3	3.2695608749645286	3.624222330239165
4	2.0415314768908197	3.017312007896319
5	1.448256992974511	2.6755913788780545
6	1.0970572680266295	2.432118318259738
7	0.8496418638818014	2.1975465956865947
8	0.7112955682740567	2.1025398663804444
9	0.5754385940021357	1.9135756896588703
>10	4.544367547666758	33.03570510496987
>50	0.18421111670787416	4.292557722644822
>100	0.04866290554580179	3.49405698886084
>500	0.003566914540509129	0.9163474167056328
>1k	0.0025477961003636633	1.8820235827798448
>5k	2.547796100363663E-4	0.6212221700062124
>10k+	2.547796100363663E-4	3.5923379311163908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38160	3.6029435368895717	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	6599	0.6230561949668314	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4405	0.4159058249475515	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	3035	0.2865548646346921	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2088	0.19714219352791998	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2078	0.19619802593439545	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1863	0.17589842267361827	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1713	0.16173590877075045	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1649	0.15569323617219352	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1065	0.1005538487103615	TruSeq Adapter, Index 14 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029930112714727314	0.0
2	0.0	0.0	0.0	0.25039324580270295	0.0
3	0.0	0.0	0.0	0.349814093400835	0.0
4	0.0	0.0	0.0	0.7138851174638903	0.0
5	0.0	0.0	0.0	1.8328181325498	0.0
6	0.0	0.0	0.0	2.29564908689552	0.0
7	0.0	0.0	0.0	2.609679228501776	0.0
8	0.0	0.0	0.0	3.0256794702086798	0.0
9	0.0	0.0	0.0	3.215079489469699	0.0
10	9.441675935245209E-5	0.0	0.0	3.926415354431073	0.0
11	9.441675935245209E-5	0.0	0.0	4.137814478621213	0.0
12	9.441675935245209E-5	0.0	0.0	4.6459654774561105	0.0
13	9.441675935245209E-5	0.0	0.0	4.77607177184379	0.0
14	9.441675935245209E-5	0.0	0.0	4.8415970028343915	0.0
15	9.441675935245209E-5	0.0	0.0	4.925439085139368	0.0
16	9.441675935245209E-5	0.0	0.0	5.017778675786067	0.0
17	9.441675935245209E-5	0.0	0.0	5.110495933470174	0.0
18	9.441675935245209E-5	0.0	0.0	5.262506916027623	0.0
19	1.8883351870490418E-4	0.0	0.0	5.35711250889878	0.0
20	2.832502780573563E-4	0.0	0.0	5.4303799141562825	0.0
21	2.832502780573563E-4	0.0	0.0	5.50459148700731	0.0
22	2.832502780573563E-4	0.0	0.0	5.5990082463597615	0.0
23	2.832502780573563E-4	0.0	0.0	5.694463590065091	0.0
24	2.832502780573563E-4	0.0	0.0	5.777455921535896	0.0
25	2.832502780573563E-4	0.0	0.0	5.841470484376859	0.0
26	3.7766703740980837E-4	0.0	0.0	5.921063812510976	0.0
27	3.7766703740980837E-4	0.0	0.0	6.0256775818734924	0.0
28	4.720837967622605E-4	0.0	0.0	6.099228237409053	0.0
29	4.720837967622605E-4	0.0	0.0	6.17844389850576	0.0
30	4.720837967622605E-4	0.0	0.0	6.276731744991663	0.0
31	4.720837967622605E-4	0.0	0.0	6.359063159147001	0.0
32	4.720837967622605E-4	0.0	0.0	6.451308333034347	0.0
33	4.720837967622605E-4	0.0	0.0	6.557149520268446	0.0
34	4.720837967622605E-4	0.0	0.0	6.667900378988872	0.0
35	4.720837967622605E-4	0.0	0.0	6.787337579569724	0.0
36	4.720837967622605E-4	0.0	0.0	6.87420099817398	0.0
37	4.720837967622605E-4	0.0	0.0	6.964841087152334	0.0
38	4.720837967622605E-4	0.0	0.0	7.081162534674555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	25	4.442603E-5	44.0	1
ATAGCGC	20	7.856201E-4	44.0	10
CTAACGG	60	0.0	44.0	2
ATATCCG	20	7.856201E-4	44.0	40
TATCGCG	20	7.856201E-4	44.0	32
TAATGCG	20	7.856201E-4	44.0	1
CGTTTTT	22120	0.0	43.353527	1
GTTTTTT	24455	0.0	39.66387	2
TCACGAC	195	0.0	39.48718	25
CGGTCTA	195	0.0	39.48718	31
TACGGGA	230	0.0	39.217392	4
CGACGGT	200	0.0	38.5	28
TGCGACG	80	0.0	38.5	1
TATAGCG	40	4.124122E-7	38.5	1
GGGCGAT	915	0.0	37.748634	7
TAGGGTA	565	0.0	37.38053	5
ATAGGGA	990	0.0	37.333332	4
ACAACGA	65	1.0913936E-11	37.23077	13
TAACGGG	190	0.0	37.05263	3
CGTTCGA	95	0.0	37.05263	14
>>END_MODULE