SRR1546100_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546100_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1057011
Sequences flagged as poor quality	0
Sequence length	50
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39039	3.693339047559581	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	6874	0.6503243580246563	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4483	0.4241204679989139	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	3107	0.2939420687201931	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2199	0.20803946221940925	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2134	0.2018900465558069	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1814	0.17161600021191833	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1665	0.15751964738304522	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1643	0.15543830669690287	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1059	0.10018817211930624	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	25	4.4425964E-5	44.0	36
TACGGGT	35	1.4459329E-7	44.0	4
TACGATG	35	1.4459329E-7	44.0	1
TATTACG	20	7.856196E-4	44.0	1
CGTTTTT	22125	0.0	43.453106	1
CGTAAGG	170	0.0	42.705883	2
CGGTCTA	150	0.0	41.066666	31
GTTTTTT	24595	0.0	39.78695	2
TCCCGCG	180	0.0	39.11111	16
TACGGGA	260	0.0	38.923077	4
GCGAGAC	85	0.0	38.823532	21
TCACGAC	170	0.0	38.823532	25
CATATGC	825	0.0	38.666668	33
GTACGAG	80	0.0	38.5	1
AGGGTAC	470	0.0	38.382977	6
TGGGTAC	330	0.0	38.000004	6
GTAGGGA	1125	0.0	37.93778	4
GCCAGTA	850	0.0	37.788235	25
ACGGGTA	70	0.0	37.714287	5
TAGCCGT	105	0.0	37.714283	44