##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546100_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1057011
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.125189804079618	31.0	28.0	31.0	25.0	33.0
2	29.054097828688633	31.0	28.0	33.0	23.0	33.0
3	28.472473796393793	31.0	28.0	33.0	16.0	34.0
4	31.995166559288407	35.0	32.0	35.0	25.0	37.0
5	33.73706517718359	35.0	33.0	35.0	30.0	37.0
6	33.75604132785752	35.0	33.0	37.0	29.0	37.0
7	34.05564180505217	35.0	33.0	37.0	30.0	37.0
8	34.57574235272859	35.0	35.0	37.0	32.0	37.0
9	36.416693866005176	39.0	35.0	39.0	32.0	39.0
10	36.07990361500495	38.0	35.0	39.0	31.0	39.0
11	36.303726262072956	38.0	35.0	39.0	31.0	39.0
12	35.4186806002965	37.0	34.0	39.0	29.0	39.0
13	35.95437701215976	38.0	35.0	39.0	30.0	39.0
14	36.94169124067772	39.0	35.0	41.0	31.0	41.0
15	37.20433183760623	39.0	36.0	41.0	32.0	41.0
16	36.84569223972125	38.0	35.0	41.0	31.0	41.0
17	36.40415473443512	38.0	34.0	40.0	30.0	41.0
18	35.91839630808005	38.0	34.0	39.0	29.0	40.0
19	35.237025915529735	37.0	34.0	39.0	27.0	40.0
20	35.23145076068272	35.0	34.0	39.0	29.0	40.0
21	35.48860324064745	36.0	34.0	39.0	30.0	40.0
22	35.87674300456665	36.0	35.0	39.0	31.0	40.0
23	36.17253935862541	37.0	35.0	40.0	31.0	41.0
24	35.80508717506251	36.0	35.0	40.0	31.0	41.0
25	35.553193864586085	36.0	35.0	40.0	30.0	41.0
26	35.53558288418947	36.0	34.0	40.0	30.0	41.0
27	35.8867779048657	37.0	35.0	40.0	31.0	41.0
28	35.74286076493055	37.0	35.0	40.0	31.0	41.0
29	35.73979457167428	37.0	35.0	40.0	31.0	41.0
30	35.32415462090744	36.0	35.0	40.0	29.0	41.0
31	34.591409171711554	36.0	34.0	40.0	25.0	41.0
32	34.379043359056816	36.0	34.0	40.0	23.0	41.0
33	33.78472977102414	36.0	33.0	40.0	19.0	41.0
34	33.0507147040097	36.0	33.0	40.0	15.0	41.0
35	32.60250460969659	36.0	32.0	40.0	10.0	41.0
36	32.19583523728703	36.0	31.0	40.0	10.0	41.0
37	32.071821390695085	36.0	31.0	40.0	10.0	41.0
38	31.878198996983002	36.0	31.0	40.0	9.0	41.0
39	31.49497308921099	35.0	30.0	40.0	8.0	40.0
40	31.229401586170816	35.0	29.0	40.0	8.0	40.0
41	30.83782193373579	35.0	27.0	39.0	8.0	40.0
42	31.00943225756402	35.0	28.0	39.0	8.0	40.0
43	30.884936864422414	35.0	28.0	39.0	8.0	40.0
44	30.75375185310276	35.0	27.0	39.0	8.0	40.0
45	30.68936841716879	35.0	27.0	39.0	8.0	40.0
46	30.370765299509657	35.0	26.0	39.0	7.0	40.0
47	30.310278700978515	35.0	26.0	38.0	7.0	40.0
48	30.294598637100275	35.0	26.0	38.0	7.0	40.0
49	30.26237002263931	35.0	26.0	38.0	7.0	40.0
50	30.442605611483703	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	5.0
11	5.0
12	12.0
13	20.0
14	50.0
15	144.0
16	305.0
17	788.0
18	1707.0
19	2672.0
20	4229.0
21	6276.0
22	9044.0
23	13173.0
24	19388.0
25	29597.0
26	41152.0
27	45599.0
28	40266.0
29	36854.0
30	36738.0
31	40536.0
32	48775.0
33	66115.0
34	92748.0
35	93549.0
36	102209.0
37	140209.0
38	154689.0
39	30154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.24910147576515	19.08106916578919	21.91453069078751	31.755298667658145
2	46.25107969548093	20.367243103430333	22.486993985871482	10.894683215217249
3	24.990846831300715	19.50991995352934	43.80645045321194	11.692782761958012
4	22.48529107076464	20.643682989107965	43.93019561764258	12.940830322484818
5	19.212193629016163	24.42349228153728	42.263798579201165	14.100515510245398
6	16.5451447525144	33.690756292980865	40.65444919683901	9.10964975766572
7	64.60954521759945	4.624739004608277	27.997343452433327	2.7683723253589605
8	64.65675380861694	5.399565378222175	26.536620716340703	3.4070600968201843
9	61.18403687378845	5.429271786197116	26.951848183226097	6.43484315678834
10	29.22911871305029	26.70416864157516	32.63343522442056	11.433277420953992
11	24.688295580651477	19.896292469993217	40.25539942346863	15.160012525886673
12	22.599859414897292	17.444567748112366	44.69461528782577	15.260957549164578
13	18.955148054277583	21.672811352010527	45.20520600069441	14.16683459301748
14	15.267958422381604	27.295742428413703	42.74383142654145	14.692467722663247
15	14.647624291516362	22.00639350016225	48.445758842623206	14.900223365698182
16	17.205213569205995	18.890342673822694	46.954194421817746	16.95024933515356
17	16.222915371741635	19.277566647839993	42.389719690712774	22.109798289705594
18	17.425646469147434	21.98056595437512	43.41118493563454	17.182602640842905
19	20.101872165947185	22.849241871655074	39.60091238407169	17.447973578326053
20	20.507638993350117	21.684164119389486	40.32569197482335	17.48250491243705
21	20.09506050551981	22.32039212458527	42.2378764270192	15.346670942875711
22	17.69763985426831	19.051362757814253	41.65737158837515	21.593625799542295
23	16.84466859852925	23.43939656257125	41.690767645748245	18.025167193151255
24	17.838603382557043	22.691154585903078	43.49396553110611	15.97627650043377
25	19.713229096007513	21.92673491571989	41.15463320627695	17.205402781995645
26	15.814310352493965	22.648297889047512	40.704401373306425	20.832990385152094
27	16.899067275553424	21.95029190803123	43.17239839509712	17.978242421318228
28	15.458684914348101	22.39711791078806	45.22507334360758	16.91912383125625
29	17.234919977180937	22.741295975160146	43.19784751530495	16.825936532353968
30	17.372761494440457	21.95369773824492	45.127439544148544	15.546101223166078
31	21.909516551861806	21.109241058040077	40.085959370337676	16.895283019760438
32	22.071388093406785	22.922278008459703	38.69543457920494	16.310899318928566
33	20.46260634941358	21.015864546348144	37.32638543969741	21.19514366454086
34	18.709549853312783	23.677047826370774	37.364038784837625	20.249363535478818
35	18.760448093728446	22.71092732242143	38.234606830014066	20.29401775383605
36	22.57545096503253	24.24392934416009	34.0700333298329	19.110586360974484
37	19.065648323432775	25.388855934328024	34.995946115981766	20.549549626257438
38	19.697240615282148	27.369913841956233	32.529557402903094	20.403288139858525
39	20.712367231750665	24.183192038682662	32.375254372944084	22.729186356622588
40	20.104804964186748	23.217639173102267	36.36707659617544	20.31047926653554
41	18.367926161600966	23.627474075482656	35.581843519130835	22.422756243785543
42	19.8804932020575	25.709382399993945	33.368526912208104	21.04159748574045
43	20.11823907225185	23.454155160163896	32.93551344309567	23.49209232448858
44	19.642085087099378	24.05727092717105	34.772485811405936	21.528158174323636
45	20.73422130895516	25.098414302216344	32.45415610622784	21.713208282600654
46	22.51215928689484	24.58867504690112	32.31262493957017	20.586540726633874
47	20.448983028558832	24.677605058036292	34.04657094391638	20.82684096948849
48	19.21664012957292	26.02366484360144	33.10826472004549	21.651430306780156
49	18.93840272239362	24.646952586113105	35.33709677571946	21.07754791577382
50	19.09809831685763	23.667681793283137	36.21059761913547	21.02362227072377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2054.0
1	2174.0
2	2294.0
3	31400.0
4	60506.0
5	41218.5
6	21931.0
7	21308.0
8	20685.0
9	20825.5
10	20966.0
11	20603.5
12	20241.0
13	19396.5
14	18552.0
15	17647.5
16	16743.0
17	15770.0
18	14797.0
19	13490.0
20	12183.0
21	11342.5
22	10502.0
23	10327.5
24	10153.0
25	9872.0
26	9591.0
27	10358.5
28	11126.0
29	12584.5
30	14043.0
31	15638.0
32	17233.0
33	19334.0
34	21435.0
35	23448.5
36	25462.0
37	27373.0
38	29284.0
39	33707.5
40	38131.0
41	44559.0
42	50987.0
43	55560.5
44	60134.0
45	71850.0
46	83566.0
47	82517.5
48	81469.0
49	81725.0
50	81981.0
51	73655.0
52	65329.0
53	55756.0
54	46183.0
55	42325.5
56	38468.0
57	36816.0
58	35164.0
59	32637.0
60	30110.0
61	27473.5
62	24837.0
63	22216.0
64	19595.0
65	16473.5
66	13352.0
67	11124.5
68	8897.0
69	7815.5
70	6734.0
71	5954.0
72	5174.0
73	4239.5
74	3305.0
75	2564.0
76	1823.0
77	1352.5
78	882.0
79	708.0
80	534.0
81	390.5
82	247.0
83	189.5
84	132.0
85	113.0
86	94.0
87	71.5
88	49.0
89	33.5
90	18.0
91	17.0
92	16.0
93	14.0
94	12.0
95	8.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1057011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.54193789667962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.35068279562934	28.265438469836525
2	7.541821549145715	5.511855493534456
3	3.373642547060212	3.6983830932081103
4	2.098969692145836	3.068016805496235
5	1.4929190647578428	2.727707787457506
6	1.1160249126152466	2.446902782876019
7	0.8918089454766652	2.281189897091815
8	0.7256056933583672	2.121203054734289
9	0.597027139126225	1.9634875776524539
>10	4.57267353747657	32.966643874630776
>50	0.18035354310203616	4.147233099133667
>100	0.05223371823133334	3.7277991467643616
>500	0.0033783001820865974	0.8953897020457411
>1k	0.002338823202983029	1.8187929752128622
>5k	2.5986924477589213E-4	0.6527636877571772
>10k+	2.5986924477589213E-4	3.707192552568001
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39039	3.693339047559581	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	6874	0.6503243580246563	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4483	0.4241204679989139	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	3107	0.2939420687201931	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2199	0.20803946221940925	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2134	0.2018900465558069	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1814	0.17161600021191833	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1665	0.15751964738304522	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1643	0.15543830669690287	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1059	0.10018817211930624	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.460639482465178E-5	0.0	0.0	0.024692269049234113	0.0
2	9.460639482465178E-5	0.0	0.0	0.23225869929452012	0.0
3	9.460639482465178E-5	0.0	0.0	0.32383768948478303	0.0
4	9.460639482465178E-5	0.0	0.0	0.6857071496890761	0.0
5	9.460639482465178E-5	0.0	0.0	1.7950617354029428	0.0
6	9.460639482465178E-5	0.0	0.0	2.2557002718041725	0.0
7	9.460639482465178E-5	0.0	0.0	2.547371787048574	0.0
8	9.460639482465178E-5	0.0	0.0	2.9550307423479985	0.0
9	9.460639482465178E-5	0.0	0.0	3.138472541912998	0.0
10	9.460639482465178E-5	0.0	0.0	3.84035738511709	0.0
11	1.8921278964930356E-4	0.0	0.0	4.045937081071058	0.0
12	2.8381918447395536E-4	0.0	0.0	4.554730272438035	0.0
13	3.784255792986071E-4	0.0	0.0	4.682448905451315	0.0
14	3.784255792986071E-4	0.0	0.0	4.752457637621557	0.0
15	3.784255792986071E-4	0.0	0.0	4.838454850517166	0.0
16	3.784255792986071E-4	0.0	0.0	4.934196522079714	0.0
17	3.784255792986071E-4	0.0	0.0	5.029181342483664	0.0
18	3.784255792986071E-4	0.0	0.0	5.1712801475102905	0.0
19	3.784255792986071E-4	0.0	0.0	5.279699075979342	0.0
20	3.784255792986071E-4	0.0	0.0	5.358411596473452	0.0
21	3.784255792986071E-4	0.0	0.0	5.4365564785986145	0.0
22	3.784255792986071E-4	0.0	0.0	5.530784447843968	0.0
23	4.730319741232589E-4	0.0	0.0	5.616687054344752	0.0
24	5.676383689479107E-4	0.0	0.0	5.7004137137645685	0.0
25	6.622447637725624E-4	0.0	0.0	5.759731923319625	0.0
26	6.622447637725624E-4	0.0	0.0	5.840052752525755	0.0
27	6.622447637725624E-4	0.0	0.0	5.93920025430199	0.0
28	7.568511585972143E-4	0.0	0.0	6.017345136427152	0.0
29	8.51457553421866E-4	0.0	0.0	6.097382146448807	0.0
30	9.460639482465178E-4	0.0	0.0	6.1978541377525875	0.0
31	9.460639482465178E-4	0.0	0.0	6.275904413482925	0.0
32	9.460639482465178E-4	0.0	0.0	6.363604541485377	0.0
33	9.460639482465178E-4	0.0	0.0	6.462941256051262	0.0
34	9.460639482465178E-4	0.0	0.0	6.571644003704787	0.0
35	9.460639482465178E-4	0.0	0.0	6.693591646633762	0.0
36	9.460639482465178E-4	0.0	0.0	6.780251104293144	0.0
37	9.460639482465178E-4	0.0	0.0	6.8661537107939274	0.0
38	9.460639482465178E-4	0.0	0.0	6.974667245657803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	25	4.4425964E-5	44.0	36
TACGGGT	35	1.4459329E-7	44.0	4
TACGATG	35	1.4459329E-7	44.0	1
TATTACG	20	7.856196E-4	44.0	1
CGTTTTT	22125	0.0	43.453106	1
CGTAAGG	170	0.0	42.705883	2
CGGTCTA	150	0.0	41.066666	31
GTTTTTT	24595	0.0	39.78695	2
TCCCGCG	180	0.0	39.11111	16
TACGGGA	260	0.0	38.923077	4
GCGAGAC	85	0.0	38.823532	21
TCACGAC	170	0.0	38.823532	25
CATATGC	825	0.0	38.666668	33
GTACGAG	80	0.0	38.5	1
AGGGTAC	470	0.0	38.382977	6
TGGGTAC	330	0.0	38.000004	6
GTAGGGA	1125	0.0	37.93778	4
GCCAGTA	850	0.0	37.788235	25
ACGGGTA	70	0.0	37.714287	5
TAGCCGT	105	0.0	37.714283	44
>>END_MODULE