SRR1546099_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546099_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1044778
Sequences flagged as poor quality	0
Sequence length	50
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38498	3.684801938785082	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	7037	0.6735402161990394	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4312	0.4127192571053372	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	2901	0.27766664305718536	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2058	0.1969796454366382	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2032	0.1944910784874873	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1897	0.1815696731745883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1644	0.15735400247708126	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1624	0.15543972020850363	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1114	0.10662552235977404	TruSeq Adapter, Index 14 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1067	0.10212695902861661	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	25	4.4425615E-5	44.0	9
TAGCCGT	65	0.0	43.999996	44
CGTTTTT	22370	0.0	43.360752	1
CGGGATC	155	0.0	42.580647	6
AGTACGG	75	0.0	41.066666	2
GTTACGG	65	0.0	40.615383	2
GTTTTTT	24790	0.0	39.70472	2
TTACGGG	240	0.0	39.416668	3
CGACGGT	190	0.0	39.36842	28
GCGGGAT	405	0.0	38.5679	5
TCGATAG	40	4.1240673E-7	38.500004	1
ATCGTAG	40	4.1240673E-7	38.500004	1
GCGTAAG	40	4.1240673E-7	38.500004	1
AGGGTAC	555	0.0	38.45045	6
TACGGGA	235	0.0	38.382977	4
TAGGGAC	970	0.0	38.329895	5
CATATGC	850	0.0	38.305885	33
CGTTCGA	115	0.0	38.260868	14
TAGGGTA	535	0.0	38.242992	5
CGGTCTA	190	0.0	38.210526	31