##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546099_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1044778
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.027774321434794	31.0	28.0	31.0	25.0	33.0
2	28.952438699896053	31.0	28.0	33.0	16.0	33.0
3	28.37171437377127	31.0	28.0	33.0	16.0	34.0
4	31.911040431555794	35.0	32.0	35.0	25.0	37.0
5	33.689215316555284	35.0	33.0	35.0	30.0	37.0
6	33.71039493557483	35.0	33.0	37.0	28.0	37.0
7	33.96043848549644	35.0	33.0	37.0	30.0	37.0
8	34.53840337373107	35.0	35.0	37.0	32.0	37.0
9	36.387165503102096	39.0	35.0	39.0	31.0	39.0
10	36.14055521842918	38.0	35.0	39.0	31.0	39.0
11	36.30872204430032	38.0	35.0	39.0	31.0	39.0
12	35.58054629787381	37.0	35.0	39.0	30.0	39.0
13	36.02044262034614	38.0	35.0	39.0	31.0	39.0
14	36.993872382458285	39.0	35.0	41.0	31.0	41.0
15	37.16276663559148	39.0	36.0	41.0	32.0	41.0
16	36.79766897848155	38.0	35.0	41.0	31.0	41.0
17	36.35015285543915	38.0	34.0	40.0	30.0	41.0
18	35.83611446642253	38.0	34.0	39.0	29.0	40.0
19	35.136954453481984	37.0	34.0	39.0	27.0	40.0
20	35.29822507748057	35.0	34.0	39.0	30.0	40.0
21	35.58658968699571	36.0	34.0	39.0	30.0	40.0
22	35.88957079877256	36.0	35.0	40.0	31.0	41.0
23	36.160823639088875	37.0	35.0	40.0	31.0	41.0
24	35.81278606555651	36.0	35.0	40.0	31.0	41.0
25	35.571119414842194	36.0	35.0	40.0	30.0	41.0
26	35.54186726749606	36.0	34.0	40.0	30.0	41.0
27	35.88361833805842	37.0	35.0	40.0	31.0	41.0
28	35.74892178051223	37.0	35.0	40.0	31.0	41.0
29	35.72763017597997	37.0	35.0	40.0	30.0	41.0
30	35.305480207278485	36.0	35.0	40.0	29.0	41.0
31	34.54657640187676	36.0	34.0	40.0	25.0	41.0
32	34.34779254540199	36.0	34.0	40.0	23.0	41.0
33	33.77593804616866	36.0	33.0	40.0	19.0	41.0
34	33.05754906784025	36.0	33.0	40.0	15.0	41.0
35	32.582121752180846	36.0	32.0	40.0	10.0	41.0
36	32.22045066033167	36.0	31.0	40.0	10.0	41.0
37	32.12023319786596	36.0	31.0	40.0	10.0	41.0
38	31.895998958630447	36.0	30.0	40.0	9.0	41.0
39	31.483623315192318	35.0	30.0	40.0	8.0	41.0
40	31.276505630861294	35.0	29.0	40.0	8.0	40.0
41	30.868717564879812	35.0	27.0	39.0	8.0	40.0
42	31.066237995057325	35.0	28.0	40.0	8.0	40.0
43	30.93510200253068	35.0	28.0	39.0	8.0	40.0
44	30.82048339455846	35.0	27.0	39.0	8.0	40.0
45	30.78272704823417	35.0	27.0	39.0	8.0	40.0
46	30.47501765925393	35.0	26.0	39.0	7.0	40.0
47	30.430278968355	35.0	26.0	39.0	7.0	40.0
48	30.387471788265067	35.0	26.0	39.0	7.0	40.0
49	30.37458866859754	35.0	26.0	39.0	7.0	40.0
50	30.475173673258816	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	9.0
12	11.0
13	15.0
14	30.0
15	136.0
16	375.0
17	797.0
18	1658.0
19	2670.0
20	4242.0
21	6235.0
22	9008.0
23	12859.0
24	19306.0
25	29175.0
26	41129.0
27	44645.0
28	40468.0
29	36463.0
30	36191.0
31	39887.0
32	48307.0
33	64683.0
34	91658.0
35	91784.0
36	99182.0
37	135987.0
38	153882.0
39	33981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.149595416442533	19.221786829355135	21.871249203180003	31.757368551022324
2	46.174402600361034	20.388350443826344	22.517510897051814	10.919736058760808
3	25.063602028373495	19.54242910934189	43.71110417715534	11.682864685129282
4	22.596953611197783	20.67013279376097	43.77800834244213	12.954905252599117
5	19.215852554322545	24.458114546822387	42.20504260235189	14.12099029650318
6	16.6440143264885	33.61278663984119	40.56402412761371	9.179174906056598
7	64.57907804337381	4.689321559221193	27.908416907706705	2.8231834896982897
8	64.7059949577805	5.452354471476236	26.410299604317856	3.4313509664254034
9	61.23645406009698	5.434838788718752	26.871545916931634	6.457161234252635
10	29.21481884189751	27.078766972505164	32.58519991806872	11.121214267528604
11	24.741428322571878	19.927965558233424	40.17753053758789	15.153075581606808
12	22.621073567781863	17.713810972283106	44.56439549837381	15.100719961561213
13	19.01743719718447	21.80003790278892	45.00669041652868	14.17583448349793
14	15.261232529781447	27.358443611944356	42.634224686966995	14.746099171307206
15	14.651533627239472	22.046788887208574	48.269201686865536	15.03247579868642
16	17.232081839395548	18.899421695326662	46.86928706385471	16.999209401423077
17	16.22813650364001	19.357892298651006	42.36411945887069	22.0498517388383
18	17.356893043306805	21.924944820813607	43.36806479462623	17.350097341253356
19	20.14303517110812	22.790008977983838	39.57721161816195	17.489744232746098
20	20.561880131472908	21.892593450474646	40.29305747249655	17.252468945555897
21	20.169165124074205	22.292678444607372	42.175275513075505	15.362880918242919
22	17.72357381185285	19.101091332321314	41.5446152196926	21.63071963613323
23	16.899283867003327	23.42736925930676	41.62511078908629	18.04823608460362
24	17.787606553736772	22.7600504604806	43.465214619756544	15.987128366026084
25	19.800952929713297	21.999984685741854	41.077434632046234	17.12162775249862
26	15.827190082486423	22.66682491400087	40.58833551242465	20.91764949108806
27	16.99480655220535	21.978449010220352	43.03009826010884	17.996646177465454
28	15.534017753053758	22.474056689555102	45.02506752630702	16.966858031084115
29	17.3227231048127	22.76521902260576	43.08609101646474	16.825966856116803
30	17.429348627172473	22.038174616999974	45.06861744791716	15.463859307910388
31	21.92829481478362	21.128603397085314	39.952698085143446	16.99040370298762
32	22.03434605246282	22.913671612533953	38.772830208905624	16.2791521260976
33	20.529720189360802	21.04590640308276	37.21852872093402	21.20584468662242
34	18.757190522771346	23.549404753928584	37.307925702876595	20.385479020423478
35	18.71048203541805	22.646342093727085	38.22477119541185	20.418404675443014
36	22.654094936914827	24.221126401972477	34.074320094795254	19.050458566317438
37	18.97848155301892	25.46569701888822	35.05424118808015	20.50158024001271
38	19.757498722216585	27.322550819408526	32.5589742509892	20.360976207385683
39	20.729092687633162	24.170972206535744	32.43339733417051	22.666537771660582
40	20.100442390632267	23.27441810604741	36.33154603178857	20.29359347153175
41	18.36313551778464	23.626646043465694	35.5462117311046	22.46400670764507
42	20.01382111797913	25.729102259044506	33.23088732725996	21.02618929571641
43	20.067038165045588	23.511119108557033	32.95657067817278	23.4652720482246
44	19.58856331201461	24.124742289749594	34.78155167892126	21.505142719314534
45	20.655201392066065	25.1514675845012	32.57514993615869	21.618181087274042
46	22.48046953515484	24.63173994858238	32.43780018338824	20.449990332874542
47	20.513353075964464	24.708215525212054	33.99018738909127	20.788244009732214
48	19.148661246695468	26.10344015666486	33.14656319332911	21.60133540331056
49	18.966804431180595	24.747745454058183	35.359952066371996	20.925498048389226
50	19.142344115209163	23.663495977135813	36.23573620424626	20.958423703408762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2070.0
1	2206.5
2	2343.0
3	30988.0
4	59633.0
5	40854.0
6	22075.0
7	21193.0
8	20311.0
9	20614.0
10	20917.0
11	20447.0
12	19977.0
13	19132.0
14	18287.0
15	17354.5
16	16422.0
17	15260.5
18	14099.0
19	13014.0
20	11929.0
21	11112.5
22	10296.0
23	9978.5
24	9661.0
25	9684.5
26	9708.0
27	10507.0
28	11306.0
29	12661.5
30	14017.0
31	15435.0
32	16853.0
33	18927.0
34	21001.0
35	23167.0
36	25333.0
37	27289.0
38	29245.0
39	33447.0
40	37649.0
41	44193.0
42	50737.0
43	54979.0
44	59221.0
45	71018.5
46	82816.0
47	81775.5
48	80735.0
49	80981.0
50	81227.0
51	72575.5
52	63924.0
53	54608.5
54	45293.0
55	41663.0
56	38033.0
57	36268.5
58	34504.0
59	32186.0
60	29868.0
61	27216.0
62	24564.0
63	22183.0
64	19802.0
65	16527.5
66	13253.0
67	11048.5
68	8844.0
69	7716.0
70	6588.0
71	5896.5
72	5205.0
73	4282.5
74	3360.0
75	2532.0
76	1704.0
77	1286.0
78	868.0
79	684.5
80	501.0
81	387.5
82	274.0
83	197.5
84	121.0
85	103.5
86	86.0
87	65.5
88	45.0
89	38.0
90	31.0
91	24.5
92	18.0
93	12.5
94	7.0
95	9.5
96	12.0
97	8.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1044778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.356452031876366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22750602744819	28.077181184283624
2	7.507950082862191	5.459248540906029
3	3.441009605183809	3.7530870195627286
4	2.1592442740462148	3.1400984349785963
5	1.485548124640506	2.700462956726822
6	1.10311322315712	2.4063169790064216
7	0.8789868881927643	2.2369791246059956
8	0.7111406275935968	2.068364009202004
9	0.6023667991749163	1.9709927675817969
>10	4.637956500494726	33.15429509337932
>50	0.18896463830380086	4.350688406994823
>100	0.04987932621092196	3.6243759880170887
>500	0.0034308531244558542	0.899521493132397
>1k	0.0023752060092386684	1.7893521413787812
>5k	2.6391177880429647E-4	0.6751928263650857
>10k+	2.6391177880429647E-4	3.693843033878509
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38498	3.684801938785082	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	7037	0.6735402161990394	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4312	0.4127192571053372	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG	2901	0.27766664305718536	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2058	0.1969796454366382	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2032	0.1944910784874873	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1897	0.1815696731745883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1644	0.15735400247708126	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1624	0.15543972020850363	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1114	0.10662552235977404	TruSeq Adapter, Index 14 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1067	0.10212695902861661	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028809948142093346	0.0
2	0.0	0.0	0.0	0.2483781243479476	0.0
3	0.0	0.0	0.0	0.3455279494782624	0.0
4	0.0	0.0	0.0	0.7140272861794563	0.0
5	0.0	0.0	0.0	1.8093796002595768	0.0
6	0.0	0.0	0.0	2.2834516040728268	0.0
7	0.0	0.0	0.0	2.578825358114355	0.0
8	0.0	0.0	0.0	2.974316074802494	0.0
9	0.0	0.0	0.0	3.1554071774099377	0.0
10	0.0	0.0	0.0	3.8456973634590317	0.0
11	9.571411342888155E-5	0.0	0.0	4.042102724215097	0.0
12	9.571411342888155E-5	0.0	0.0	4.532828983764972	0.0
13	9.571411342888155E-5	0.0	0.0	4.658214472356807	0.0
14	9.571411342888155E-5	0.0	0.0	4.725884350551026	0.0
15	9.571411342888155E-5	0.0	0.0	4.813367050225024	0.0
16	9.571411342888155E-5	0.0	0.0	4.9071668813853275	0.0
17	9.571411342888155E-5	0.0	0.0	4.995223865739899	0.0
18	1.914282268577631E-4	0.0	0.0	5.127309342271755	0.0
19	2.871423402866446E-4	0.0	0.0	5.226373449670648	0.0
20	5.742846805732892E-4	0.0	0.0	5.305911877930049	0.0
21	5.742846805732892E-4	0.0	0.0	5.3806646005180045	0.0
22	5.742846805732892E-4	0.0	0.0	5.471880150615729	0.0
23	5.742846805732892E-4	0.0	0.0	5.557639996248007	0.0
24	5.742846805732892E-4	0.0	0.0	5.640432704363989	0.0
25	5.742846805732892E-4	0.0	0.0	5.6948940349050226	0.0
26	5.742846805732892E-4	0.0	0.0	5.768402474018404	0.0
27	5.742846805732892E-4	0.0	0.0	5.869955148366447	0.0
28	5.742846805732892E-4	0.0	0.0	5.943750729820115	0.0
29	5.742846805732892E-4	0.0	0.0	6.021470589924367	0.0
30	5.742846805732892E-4	0.0	0.0	6.114791850517526	0.0
31	6.699987940021708E-4	0.0	0.0	6.192033140054634	0.0
32	7.657129074310524E-4	0.0	0.0	6.283535832492644	0.0
33	7.657129074310524E-4	0.0	0.0	6.383844223366112	0.0
34	7.657129074310524E-4	0.0	0.0	6.490278317499029	0.0
35	8.614270208599339E-4	0.0	0.0	6.611069528646277	0.0
36	8.614270208599339E-4	0.0	0.0	6.696542231938269	0.0
37	9.571411342888154E-4	0.0	0.0	6.781919221116831	0.0
38	9.571411342888154E-4	0.0	0.0	6.887396174115458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	25	4.4425615E-5	44.0	9
TAGCCGT	65	0.0	43.999996	44
CGTTTTT	22370	0.0	43.360752	1
CGGGATC	155	0.0	42.580647	6
AGTACGG	75	0.0	41.066666	2
GTTACGG	65	0.0	40.615383	2
GTTTTTT	24790	0.0	39.70472	2
TTACGGG	240	0.0	39.416668	3
CGACGGT	190	0.0	39.36842	28
GCGGGAT	405	0.0	38.5679	5
TCGATAG	40	4.1240673E-7	38.500004	1
ATCGTAG	40	4.1240673E-7	38.500004	1
GCGTAAG	40	4.1240673E-7	38.500004	1
AGGGTAC	555	0.0	38.45045	6
TACGGGA	235	0.0	38.382977	4
TAGGGAC	970	0.0	38.329895	5
CATATGC	850	0.0	38.305885	33
CGTTCGA	115	0.0	38.260868	14
TAGGGTA	535	0.0	38.242992	5
CGGTCTA	190	0.0	38.210526	31
>>END_MODULE