FastQCFastQC Report
Thu 26 May 2016
SRR1546098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546098_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences920984
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200782.1800595884401903No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCATTGCGTCTCGTATG16630.18056774059049885No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12980.14093621604718432No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12130.13170695690696038No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC11640.12638656046141952No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCATTGCGTCTCGTATGCC10420.11313985910721576No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT9930.10781946266167491TruSeq Adapter, Index 14 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACT207.855666E-444.08
CGGAGAT207.855666E-444.027
CGTTTTT208450.043.4722981
GCGTAAG750.041.0666661
CGTAAGG1450.040.965522
TCACGAC1200.040.33333225
GTTTTTT230400.039.6649322
CGTTTAC1000.039.633
GGTACCT6850.039.182488
GACGTAG452.3501343E-839.111111
GACGGTC1800.039.1111129
CGGTCTA1800.039.1111131
ACGGGAT3050.038.950825
GGGTACC6950.038.9352537
TAGCCGT1250.038.7244
ACTAACC1650.038.66666814
TAGGGAC7700.038.571435
GTAGGGA9050.038.408844
ACGGGCG1950.038.3589745
GCCAGTA6850.038.21897525
Produced by FastQC (version 0.11.5)