##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546098_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	920984
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.007755835063367	28.0	25.0	31.0	16.0	33.0
2	26.267126247578677	30.0	25.0	31.0	16.0	33.0
3	26.539892115389627	30.0	25.0	31.0	16.0	33.0
4	27.31624110733737	32.0	19.0	35.0	10.0	35.0
5	31.369667659807337	33.0	32.0	35.0	19.0	35.0
6	30.44893179468916	35.0	28.0	35.0	17.0	36.0
7	30.288105982297196	32.0	28.0	35.0	18.0	35.0
8	32.494951052352704	35.0	32.0	35.0	28.0	35.0
9	27.187416936667738	30.0	23.0	34.0	10.0	37.0
10	31.272953710379333	34.0	28.0	37.0	17.0	39.0
11	33.501593947343274	35.0	32.0	38.0	26.0	39.0
12	33.28306897839702	35.0	31.0	39.0	22.0	39.0
13	33.91578355324305	37.0	32.0	39.0	25.0	39.0
14	34.12857443777525	36.0	32.0	39.0	25.0	41.0
15	32.40007101100562	35.0	27.0	39.0	18.0	40.0
16	31.038975704246763	34.0	27.0	38.0	17.0	40.0
17	31.34073338950514	34.0	27.0	38.0	17.0	40.0
18	32.125966357721744	35.0	29.0	38.0	17.0	39.0
19	31.687711187165032	35.0	29.0	37.0	18.0	39.0
20	31.864594824665794	34.0	30.0	37.0	18.0	39.0
21	32.97794532804044	35.0	31.0	37.0	25.0	39.0
22	33.34929488460169	35.0	31.0	37.0	26.0	39.0
23	34.34058137817812	35.0	33.0	38.0	28.0	40.0
24	34.02211547649036	35.0	33.0	38.0	26.0	40.0
25	33.03465966835471	35.0	31.0	38.0	24.0	40.0
26	26.34851745524352	33.0	19.0	36.0	0.0	39.0
27	29.77971061386517	34.0	24.0	37.0	15.0	39.0
28	31.244784925688176	34.0	27.0	37.0	19.0	39.0
29	32.319567983808625	35.0	30.0	37.0	22.0	39.0
30	32.19068735178896	35.0	30.0	38.0	21.0	39.0
31	30.982138669075685	34.0	29.0	37.0	18.0	39.0
32	31.16218957115433	34.0	30.0	37.0	16.0	39.0
33	30.603071280282826	34.0	28.0	37.0	15.0	39.0
34	30.057072652727953	34.0	26.0	37.0	10.0	39.0
35	29.018707165379638	33.0	24.0	37.0	8.0	39.0
36	28.868470027709492	33.0	23.0	37.0	8.0	39.0
37	28.965199178270197	34.0	23.0	37.0	8.0	39.0
38	28.64253342077604	33.0	23.0	37.0	8.0	39.0
39	28.382953449788488	33.0	22.0	37.0	7.0	39.0
40	28.14688745949984	33.0	22.0	37.0	7.0	39.0
41	27.39988425423243	32.0	20.0	36.0	7.0	38.0
42	27.57447143490006	33.0	20.0	36.0	7.0	38.0
43	27.32712294676129	32.0	20.0	36.0	7.0	38.0
44	27.15047384102221	32.0	19.0	36.0	7.0	38.0
45	26.664009363897744	31.0	19.0	35.0	7.0	38.0
46	26.072495287648863	30.0	18.0	35.0	7.0	38.0
47	26.04305069360597	30.0	18.0	34.0	7.0	37.0
48	26.06601851932281	31.0	18.0	34.0	7.0	37.0
49	25.86223756330186	30.0	18.0	34.0	7.0	37.0
50	27.52179951008921	33.0	20.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	11.0
11	17.0
12	41.0
13	136.0
14	398.0
15	1045.0
16	1961.0
17	3442.0
18	5402.0
19	8164.0
20	11913.0
21	16545.0
22	22711.0
23	29565.0
24	38938.0
25	47589.0
26	53822.0
27	54929.0
28	55522.0
29	59119.0
30	67645.0
31	77691.0
32	86391.0
33	90880.0
34	84857.0
35	64889.0
36	31183.0
37	6031.0
38	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.99219964733372	19.491652406556465	21.31090225237355	33.20524569373626
2	46.78626338785473	18.22941549473172	23.30876540743379	11.675555709979761
3	26.038671681592728	15.966075414990923	45.51827176150726	12.476981141909087
4	23.46609713089478	20.27114477558785	43.319536495748025	12.943221597769341
5	18.607706539961608	24.270671368883715	43.329525811523325	13.79209627963135
6	16.44187086854929	32.465276269728896	41.96707000338768	9.125782858334128
7	66.03306897839701	3.026979838954857	28.932098711812582	2.0078524708355414
8	66.9733676155069	4.074772200168515	26.409579319510435	2.5422808648141553
9	55.28054776195896	3.3931099780235057	24.384136966548823	16.942205293468724
10	29.252951191334486	27.375394143655047	33.09525464068865	10.276400024321813
11	24.831267427012847	19.42802480824857	41.2009329152298	14.539774849508785
12	22.299953093647666	17.199973072279214	45.95324131581005	14.546832518263075
13	18.324531153635675	21.761398677935773	46.123385422548054	13.790684745880494
14	14.779952746193203	27.061165014810246	43.7718787731383	14.387003465858255
15	13.095232924784794	20.236073590855	47.05260894868966	19.616084535670545
16	15.35976737923786	16.436659051623046	46.33468116709954	21.86889240203956
17	15.379311692711273	18.43332783197102	43.394890682139966	22.79246979317773
18	16.599202592010283	21.47768039401336	44.38133561495097	17.541781399025393
19	19.94019440077135	22.314177010675536	40.69972985415599	17.04589873439712
20	20.09459447721133	21.320348670552367	41.5385066407234	17.046550211512905
21	19.923038836722462	21.426865178982478	43.187829538841065	15.462266445453993
22	17.156432685041217	18.552222405600965	42.645583419473084	21.645761489884734
23	16.20896779965776	23.334933071584306	42.717463061247535	17.738636067510402
24	17.12483604492586	22.388771140432407	44.99133535436012	15.495057460281611
25	19.325634321551732	20.717189440858906	42.62386751561373	17.333308721975627
26	12.643650704029602	35.143390113183294	34.06150378291045	18.151455399876653
27	15.809069430087819	22.21287231917167	44.47753706904789	17.500521181692623
28	14.678756634208629	22.086377179190954	46.84207326077326	16.39279292582716
29	16.7825934001025	22.302341843072192	44.36396289186349	16.551101864961822
30	16.819076118586207	21.164211321803634	46.53283878981611	15.483873769794046
31	22.265533386030594	20.570498510288996	40.75532256803593	16.408645535644485
32	21.33793855267844	22.42818550593713	39.619363637153306	16.614512304231127
33	19.915655429410283	20.43119098703126	38.09979326459526	21.553360318963197
34	18.169045282002728	22.924176750084694	37.87481650061239	21.031961467300192
35	17.9024825621292	22.157171025772435	38.246701354203765	21.6936450578946
36	22.338390243478713	23.40594407720438	34.14988751161801	20.105778167698894
37	18.21508299818455	25.051032374069475	34.85619728464338	21.877687343102593
38	19.18806407060275	27.43674157205771	32.555505850264495	20.81968850707504
39	19.744425527479304	23.637435612345055	32.612292938856704	24.00584592131894
40	19.396102429575322	22.51407190570086	36.59194948012126	21.497876184602553
41	17.818984911789997	22.564452802654554	36.00323132649427	23.613330959061177
42	19.271127402864764	25.04810072704846	32.500130295423155	23.18064157466362
43	19.503378994640517	22.774337013455174	32.80013550724008	24.922148484664227
44	18.89240203955769	23.43037446904615	34.779974462097066	22.897249029299097
45	20.363980264586573	25.178287570685264	31.891759248803453	22.565972915924707
46	21.67833534567376	23.509854677171376	32.116301694709136	22.69550828244573
47	19.981671777142708	23.767948194539752	33.284291583784295	22.966088444533238
48	18.29901496660094	25.60902252373548	33.01077977467578	23.081182734987795
49	18.231261346559766	24.37045594711743	34.258467030914765	23.139815675408045
50	18.559931551471035	23.239383094603163	36.18673071410578	22.013954639820017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1162.0
1	1369.5
2	1577.0
3	18534.0
4	35491.0
5	27799.0
6	20107.0
7	19258.5
8	18410.0
9	18828.5
10	19247.0
11	19528.5
12	19810.0
13	19588.0
14	19366.0
15	18694.0
16	18022.0
17	17300.0
18	16578.0
19	15451.5
20	14325.0
21	13461.0
22	12597.0
23	11981.0
24	11365.0
25	11116.5
26	10868.0
27	11162.0
28	11456.0
29	12600.0
30	13744.0
31	14954.5
32	16165.0
33	17715.5
34	19266.0
35	20617.5
36	21969.0
37	23453.0
38	24937.0
39	27932.0
40	30927.0
41	35981.5
42	41036.0
43	45156.0
44	49276.0
45	54609.0
46	59942.0
47	64152.0
48	68362.0
49	68914.5
50	69467.0
51	63927.5
52	58388.0
53	51023.5
54	43659.0
55	39510.5
56	35362.0
57	33494.0
58	31626.0
59	29324.0
60	27022.0
61	24556.0
62	22090.0
63	19976.5
64	17863.0
65	15121.5
66	12380.0
67	10461.0
68	8542.0
69	7454.5
70	6367.0
71	5649.5
72	4932.0
73	4130.5
74	3329.0
75	2527.0
76	1725.0
77	1346.5
78	968.0
79	762.0
80	556.0
81	430.5
82	305.0
83	248.5
84	192.0
85	144.0
86	96.0
87	74.5
88	53.0
89	42.5
90	32.0
91	22.0
92	12.0
93	9.0
94	6.0
95	5.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	920984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.75207028721767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72066716564449	45.00159185980478
2	6.882602810137117	7.399083000189844
3	2.690301266813844	4.338277883627055
4	1.4863137430421367	3.1956976313943404
5	0.960992597328162	2.582767081853962
6	0.6895889700572835	2.2240100872685487
7	0.5207954711555588	1.9595684339572765
8	0.39640393740736973	1.7046025844520616
9	0.33316344736682446	1.6117402535993963
>10	2.240092168957708	21.573603637334063
>50	0.051143760064679945	1.8281283100079921
>100	0.024711431794705762	2.651730087580479
>500	0.0019339381380947557	0.8732067013320015
>1k	0.0010744100767193087	0.7369125336637791
>5k	0.0	0.0
>10k+	2.148820153438617E-4	2.3190799139343814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20078	2.1800595884401903	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCATTGCGTCTCGTATG	1663	0.18056774059049885	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1298	0.14093621604718432	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1213	0.13170695690696038	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	1164	0.12638656046141952	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCATTGCGTCTCGTATGCC	1042	0.11313985910721576	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	993	0.10781946266167491	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024756130399659494	0.0
2	0.0	0.0	0.0	0.15635450778732313	0.0
3	0.0	0.0	0.0	0.2934904406591211	0.0
4	0.0	0.0	0.0	0.4091276287101622	0.0
5	0.0	0.0	0.0	0.9784100483830338	0.0
6	0.0	0.0	0.0	1.1718987517698463	0.0
7	0.0	0.0	0.0	1.2763522493333217	0.0
8	0.0	0.0	0.0	1.4529025477098407	0.0
9	0.0	0.0	0.0	1.5892784239465616	0.0
10	0.0	0.0	0.0	2.005355141891716	0.0
11	0.0	0.0	0.0	2.1554120375598274	0.0
12	0.0	0.0	0.0	2.457805998801282	0.0
13	0.0	0.0	0.0	2.5422808648141553	0.0
14	0.0	0.0	0.0	2.5791979013750512	0.0
15	0.0	0.0	0.0	2.630773173041008	0.0
16	0.0	0.0	0.0	2.696680941254137	0.0
17	0.0	0.0	0.0	2.766171833604058	0.0
18	0.0	0.0	0.0	2.8650877756833997	0.0
19	0.0	0.0	0.0	2.9509741754471306	0.0
20	0.0	0.0	0.0	3.0048296170183195	0.0
21	0.0	0.0	0.0	3.0531475031053743	0.0
22	0.0	0.0	0.0	3.1109118073712465	0.0
23	0.0	0.0	0.0	3.17030480442657	0.0
24	0.0	0.0	0.0	3.22112001945745	0.0
25	0.0	0.0	0.0	3.258037056018346	0.0
26	0.0	0.0	0.0	3.3065721011439937	0.0
27	0.0	0.0	0.0	3.383989298402578	0.0
28	0.0	0.0	0.0	3.444359511131572	0.0
29	0.0	0.0	0.0	3.5067927347272048	0.0
30	0.0	0.0	0.0	3.5758493089999392	0.0
31	0.0	0.0	0.0	3.6336136132658114	0.0
32	0.0	0.0	0.0	3.701692971864875	0.0
33	0.0	0.0	0.0	3.77574420402526	0.0
34	0.0	0.0	0.0	3.855550150708373	0.0
35	0.0	0.0	0.0	3.9513172867281083	0.0
36	0.0	0.0	0.0	4.020482440520139	0.0
37	0.0	0.0	0.0	4.08932185575428	0.0
38	0.0	0.0	0.0	4.1768369483074625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACT	20	7.855666E-4	44.0	8
CGGAGAT	20	7.855666E-4	44.0	27
CGTTTTT	20845	0.0	43.472298	1
GCGTAAG	75	0.0	41.066666	1
CGTAAGG	145	0.0	40.96552	2
TCACGAC	120	0.0	40.333332	25
GTTTTTT	23040	0.0	39.664932	2
CGTTTAC	100	0.0	39.6	33
GGTACCT	685	0.0	39.18248	8
GACGTAG	45	2.3501343E-8	39.11111	1
GACGGTC	180	0.0	39.11111	29
CGGTCTA	180	0.0	39.11111	31
ACGGGAT	305	0.0	38.95082	5
GGGTACC	695	0.0	38.935253	7
TAGCCGT	125	0.0	38.72	44
ACTAACC	165	0.0	38.666668	14
TAGGGAC	770	0.0	38.57143	5
GTAGGGA	905	0.0	38.40884	4
ACGGGCG	195	0.0	38.358974	5
GCCAGTA	685	0.0	38.218975	25
>>END_MODULE