FastQCFastQC Report
Thu 26 May 2016
SRR1546097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546097_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1758943
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469192.6674542608828142No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT42140.2395756997242094No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT37580.21365103928893658No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31990.18187058932552105No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT25810.14673585215666454No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC23630.13434204519418763No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT20600.11711579056285508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATCG302.5284862E-644.09
TACGTAG502.7284841E-1144.01
CGTTTTT289700.043.392481
GCCCGAA3150.041.2063525
ACGGGAC4800.039.8755
ATAGCGG2600.039.7692342
GCGATAT1500.039.69
CGTTCGA1450.039.44827314
GTTTTTT327050.039.318152
CGGTCTA2250.039.1111131
ACTTCGC452.3521352E-839.11110732
ATCGTAG800.038.51
GCCCTAC6250.038.36826
GGCGATA4650.038.3225828
CACAACG1450.037.93103412
ATAACGC1050.037.71428711
CTAGCGG2450.037.7142872
CCCTACA6450.037.51938227
TAGGGTA8600.037.348845
AGTACGG1650.037.3333322