##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546097_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1758943
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.05023642039566	31.0	28.0	33.0	25.0	33.0
2	28.976438690736426	31.0	28.0	33.0	16.0	33.0
3	28.439811295761146	31.0	28.0	33.0	16.0	34.0
4	32.14192728246453	35.0	32.0	35.0	25.0	37.0
5	33.730211837450106	35.0	33.0	35.0	30.0	37.0
6	33.57260525213154	35.0	33.0	37.0	28.0	37.0
7	34.19439856777622	35.0	33.0	37.0	30.0	37.0
8	34.69463706328175	35.0	35.0	37.0	32.0	37.0
9	36.472012452933384	39.0	37.0	39.0	32.0	39.0
10	36.14765799687653	38.0	35.0	39.0	31.0	39.0
11	36.354030801452915	38.0	35.0	39.0	32.0	39.0
12	35.371756788025536	37.0	34.0	39.0	29.0	39.0
13	35.86636462921197	37.0	35.0	39.0	30.0	39.0
14	36.65950346315941	38.0	35.0	40.0	31.0	41.0
15	37.058869446025255	39.0	35.0	40.0	32.0	41.0
16	36.66331086339921	38.0	35.0	40.0	31.0	41.0
17	36.18106101221017	38.0	34.0	40.0	30.0	41.0
18	35.81363978252848	38.0	34.0	39.0	29.0	40.0
19	35.25123611168753	37.0	34.0	39.0	28.0	41.0
20	35.43401122151201	36.0	34.0	39.0	30.0	41.0
21	35.73057796642643	36.0	34.0	39.0	31.0	41.0
22	36.01106914777795	37.0	35.0	40.0	31.0	41.0
23	36.30960923691103	37.0	35.0	40.0	32.0	41.0
24	35.99174902199787	37.0	35.0	40.0	31.0	41.0
25	35.68788186996395	36.0	35.0	40.0	30.0	41.0
26	35.761721670344066	37.0	34.0	40.0	31.0	41.0
27	36.05959942988488	37.0	35.0	40.0	31.0	41.0
28	35.944680981703215	37.0	35.0	40.0	31.0	41.0
29	35.98736911884012	37.0	35.0	40.0	31.0	41.0
30	35.563607234572125	37.0	35.0	40.0	30.0	41.0
31	34.86264705564649	36.0	34.0	40.0	27.0	41.0
32	34.69527892603683	37.0	34.0	40.0	25.0	41.0
33	34.221173170477954	37.0	33.0	40.0	22.0	41.0
34	33.73379069134133	37.0	33.0	40.0	18.0	41.0
35	33.346286377671134	37.0	33.0	40.0	15.0	41.0
36	32.93801277244345	36.0	33.0	40.0	12.0	41.0
37	32.85249038769307	37.0	33.0	40.0	10.0	41.0
38	32.655628977175496	36.0	32.0	40.0	10.0	41.0
39	32.46660920791634	36.0	32.0	40.0	10.0	41.0
40	32.27884871766737	36.0	31.0	40.0	10.0	41.0
41	31.942072597008544	35.0	31.0	40.0	10.0	40.0
42	32.12836231759642	36.0	31.0	40.0	10.0	41.0
43	31.91942490461601	35.0	31.0	40.0	10.0	40.0
44	31.66441038737469	35.0	30.0	39.0	9.0	40.0
45	31.61823094892785	35.0	30.0	39.0	10.0	40.0
46	31.470769092574347	35.0	30.0	39.0	10.0	40.0
47	31.430255556888426	35.0	30.0	39.0	10.0	40.0
48	31.40691995135715	35.0	30.0	39.0	10.0	40.0
49	31.376256081066867	35.0	30.0	39.0	9.0	40.0
50	31.546017693580747	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	3.0
12	24.0
13	32.0
14	71.0
15	190.0
16	508.0
17	1093.0
18	2254.0
19	3837.0
20	5984.0
21	9146.0
22	13018.0
23	18717.0
24	27611.0
25	42524.0
26	59707.0
27	64933.0
28	58656.0
29	53618.0
30	54935.0
31	63497.0
32	80822.0
33	115682.0
34	172645.0
35	156590.0
36	171226.0
37	241893.0
38	277726.0
39	61993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.27922394301578	21.10983698732705	24.63047409722771	26.980464972429463
2	43.42153213606126	19.0193769781056	25.852514834193034	11.706576051640104
3	27.489122728820657	17.61495398088511	42.35538047566067	12.540542814633563
4	24.459917120679865	22.64598682276799	39.27171033967559	13.622385716876556
5	19.080493228035248	26.743788741306567	39.64847070086978	14.5272473297884
6	17.604208891362596	35.209384272258966	37.54061388004046	9.645792956337983
7	70.84436505333032	4.762689865447601	21.71639444825671	2.676550632965366
8	72.03610350079565	3.761463560786222	21.226327402309227	2.9761055361089017
9	68.05945388793155	5.272655225325664	22.32124633942089	4.346644547321886
10	33.60774055782365	26.9727330561593	28.473463892803803	10.946062493213253
11	28.653515207712815	20.706128623838293	35.66806883452164	14.97228733392725
12	26.504611007860973	18.998910140919858	40.331778801245974	14.164700049973195
13	19.625707029733196	25.926138595736187	40.11795720498049	14.330197169550122
14	15.468210169402873	30.600195685704424	39.27074385014182	14.660850294750883
15	14.749994741159888	22.98454242121547	47.32506965831183	14.940393179312803
16	16.637605652940433	18.99453251185513	47.43706873957826	16.93079309562618
17	16.348454725366313	18.797880317895462	39.3184429512497	25.53522200548852
18	18.50315786242078	22.579014783310203	41.63466354509498	17.28316380917403
19	22.16956433494434	23.631010214657326	36.86424176337721	17.335183687021125
20	22.699030042474373	21.979848124697618	36.88823344474494	18.43288838808307
21	21.177377550039996	22.89386296201753	39.17165024676752	16.757109241174955
22	18.647847030858873	20.41413507998838	37.76603335071119	23.171984538441553
23	17.194872147647764	25.505260829941616	37.473869249884736	19.825997772525884
24	17.120964124477027	23.247996097656376	43.22578957930985	16.405250198556747
25	20.229819840665673	21.69575705409442	40.0274483027591	18.046974802480808
26	16.031673567591444	25.145101347798082	37.421394553433515	21.401830531176962
27	16.1487893581543	24.627745185602944	40.50063020802834	18.722835248214412
28	15.296857260297802	23.539477970576648	44.2821626397217	16.881502129403852
29	16.596671978568946	23.867231627176093	41.55040839868035	17.985687995574615
30	17.78073536208962	24.215565825612316	41.678382983416746	16.325315828881322
31	23.755175693584157	23.41440285444156	35.93481994584247	16.895601506131808
32	22.480432850865547	24.609097622833715	35.90406283773835	17.006406688562393
33	21.029447799047496	22.945882839864623	34.76008034370642	21.26458901738146
34	17.619104200647776	24.507843631089806	35.51524978353477	22.357802384727645
35	18.213154149963927	24.022267918858088	36.37735844765862	21.387219483519363
36	24.525183590372173	24.565946707767107	31.998194370141615	18.910675331719105
37	18.532777924014592	27.66445530071185	33.05274815613695	20.750018619136608
38	19.598361061160027	29.2052670268451	31.021869384056224	20.174502527938653
39	19.35139455911874	25.54818433570616	31.528366752077808	23.572054353097286
40	20.496570951986506	23.657275989045694	35.03513189455258	20.81102116441522
41	17.901489701485495	23.538113514764262	34.71789591817359	23.842500865576653
42	20.45683117645086	26.0926590571724	30.50400155093144	22.9465082154453
43	19.706323627314816	24.55906757637968	31.405167762684748	24.32944103362076
44	19.385903920706923	25.921817818996978	33.20681795828517	21.48546030201092
45	20.15784479656248	28.42616275797453	30.164991133879838	21.251001311583153
46	22.21697917442464	26.52900065550731	30.510937534644384	20.74308263542366
47	21.0041485141929	25.643184571643314	31.517223696276687	21.835443217887107
48	18.955986635155316	27.001557185195878	32.544602070675396	21.4978541089734
49	19.293803153371087	26.010564299127374	33.30557044770638	21.390062099795163
50	18.724483965654372	26.031258545615177	34.22862480478332	21.015632683947118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1824.0
1	2317.5
2	2811.0
3	37211.5
4	71612.0
5	49737.0
6	27862.0
7	27644.5
8	27427.0
9	28144.5
10	28862.0
11	28619.0
12	28376.0
13	27434.5
14	26493.0
15	24938.0
16	23383.0
17	21784.5
18	20186.0
19	18994.5
20	17803.0
21	16593.0
22	15383.0
23	14979.0
24	14575.0
25	14745.0
26	14915.0
27	16745.5
28	18576.0
29	19930.0
30	21284.0
31	24921.5
32	28559.0
33	34090.0
34	39621.0
35	42944.5
36	46268.0
37	51729.0
38	57190.0
39	65364.5
40	73539.0
41	89459.5
42	105380.0
43	120845.5
44	136311.0
45	140280.0
46	144249.0
47	144078.5
48	143908.0
49	143934.5
50	143961.0
51	127285.5
52	110610.0
53	96289.5
54	81969.0
55	72772.5
56	63576.0
57	59785.0
58	55994.0
59	51674.5
60	47355.0
61	41530.5
62	35706.0
63	31661.5
64	27617.0
65	22489.5
66	17362.0
67	14814.0
68	12266.0
69	10805.5
70	9345.0
71	8104.0
72	6863.0
73	5776.5
74	4690.0
75	3607.0
76	2524.0
77	1961.5
78	1399.0
79	1025.5
80	652.0
81	484.5
82	317.0
83	233.0
84	149.0
85	125.5
86	102.0
87	70.5
88	39.0
89	32.0
90	25.0
91	16.5
92	8.0
93	9.0
94	10.0
95	7.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1758943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.797030164496075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96960170773272	23.232625739990564
2	7.223202939458698	4.30459991742655
3	3.0331472474242087	2.7113634007457215
4	1.8173543327273365	2.166070474874163
5	1.3113335129452974	1.9536922120472815
6	0.9654583257308497	1.7260674512619603
7	0.7691619027878251	1.6043118293125016
8	0.6299517460845739	1.5016552944207218
9	0.5080790376724165	1.3625321770325818
>10	4.828355914843672	30.819995439673075
>50	0.7791893718506628	16.040965796475977
>100	0.15566510639616493	6.747219808551436
>500	0.0062033334502001	1.3281974922016566
>1k	0.00310166672510005	1.7905308281841763
>5k	0.0	0.0
>10k+	1.9385417031875314E-4	2.7101721378015795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46919	2.6674542608828142	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4214	0.2395756997242094	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3758	0.21365103928893658	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3199	0.18187058932552105	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2581	0.14673585215666454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	2363	0.13434204519418763	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	2060	0.11711579056285508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05082597901125847	0.0
2	0.0	0.0	0.0	0.21001249045591586	0.0
3	0.0	0.0	0.0	0.29688284384428604	0.0
4	0.0	0.0	0.0	0.46374441923359655	0.0
5	0.0	0.0	0.0	0.8924678059493685	0.0
6	5.6852325515949065E-5	0.0	0.0	1.2551856427411234	0.0
7	5.6852325515949065E-5	0.0	0.0	1.492373544793663	0.0
8	5.6852325515949065E-5	0.0	0.0	1.9216654547645944	0.0
9	5.6852325515949065E-5	0.0	0.0	2.121046560349028	0.0
10	1.1370465103189813E-4	0.0	0.0	2.5616520830976333	0.0
11	1.1370465103189813E-4	0.0	0.0	2.847448723466309	0.0
12	1.1370465103189813E-4	0.0	0.0	3.1657080417045917	0.0
13	1.705569765478472E-4	0.0	0.0	3.2875425752852707	0.0
14	2.8426162757974534E-4	0.0	0.0	3.343257854290901	0.0
15	3.9796627861164344E-4	0.0	0.0	3.4148349321154807	0.0
16	5.685232551594907E-4	0.0	0.0	3.5392846726698934	0.0
17	5.685232551594907E-4	0.0	0.0	3.6911372341229933	0.0
18	6.253755806754398E-4	0.0	0.0	3.9326459129147446	0.0
19	6.253755806754398E-4	0.0	0.0	4.039755694186793	0.0
20	6.822279061913888E-4	0.0	0.0	4.1470360324353885	0.0
21	6.822279061913888E-4	0.0	0.0	4.275806549729014	0.0
22	6.822279061913888E-4	0.0	0.0	4.416800317008567	0.0
23	6.822279061913888E-4	0.0	0.0	4.576498499382868	0.0
24	7.390802317073379E-4	0.0	0.0	4.703734003887562	0.0
25	7.390802317073379E-4	0.0	0.0	4.806466156094882	0.0
26	7.390802317073379E-4	0.0	0.0	4.904365860633346	0.0
27	7.390802317073379E-4	0.0	0.0	5.010622857022655	0.0
28	7.390802317073379E-4	0.0	0.0	5.132173128975754	0.0
29	7.390802317073379E-4	0.0	0.0	5.250653375350992	0.0
30	7.390802317073379E-4	0.0	0.0	5.402847050757187	0.0
31	7.390802317073379E-4	0.0	0.0	5.531674420376328	0.0
32	7.390802317073379E-4	0.0	0.0	5.65908048185757	0.0
33	7.959325572232869E-4	0.0	0.0	5.818608107255323	0.0
34	7.959325572232869E-4	0.0	0.0	5.964036355925121	0.0
35	7.959325572232869E-4	0.0	0.0	6.157106853377284	0.0
36	7.959325572232869E-4	0.0	0.0	6.297020426472034	0.0
37	7.959325572232869E-4	0.0	0.0	6.434034530965472	0.0
38	7.959325572232869E-4	0.0	0.0	6.594983464501124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAATCG	30	2.5284862E-6	44.0	9
TACGTAG	50	2.7284841E-11	44.0	1
CGTTTTT	28970	0.0	43.39248	1
GCCCGAA	315	0.0	41.20635	25
ACGGGAC	480	0.0	39.875	5
ATAGCGG	260	0.0	39.769234	2
GCGATAT	150	0.0	39.6	9
CGTTCGA	145	0.0	39.448273	14
GTTTTTT	32705	0.0	39.31815	2
CGGTCTA	225	0.0	39.11111	31
ACTTCGC	45	2.3521352E-8	39.111107	32
ATCGTAG	80	0.0	38.5	1
GCCCTAC	625	0.0	38.368	26
GGCGATA	465	0.0	38.322582	8
CACAACG	145	0.0	37.931034	12
ATAACGC	105	0.0	37.714287	11
CTAGCGG	245	0.0	37.714287	2
CCCTACA	645	0.0	37.519382	27
TAGGGTA	860	0.0	37.34884	5
AGTACGG	165	0.0	37.333332	2
>>END_MODULE