FastQCFastQC Report
Thu 26 May 2016
SRR1546096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546096_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1757147
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477912.7198065955779454No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT41790.23782870755833177No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT36930.210170236183996No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31580.179723153498256No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT26720.1520646821239202No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC22750.1294712394580533No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT21190.12059321160950108No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATG17620.10027618634069888No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT207.85762E-444.030
ATCGACG254.4438035E-544.015
CGTTTTT299100.043.4189221
TTGCACG1350.040.740741
CGGTCTA2500.040.4831
GTTTTTT333450.039.6917082
GTTGATC13150.039.3155916
CGACGGT2800.038.49999628
CACGACG2700.038.29629526
TGCACGG2700.038.2962952
AGGGTAC9100.038.1978036
ACGATAG750.038.133331
TCGTCCC4000.037.9538
GCCAGTA13450.037.94795625
TGCGTAG1050.037.7142871
CCATACG357.2924086E-637.71428334
CCCGATT357.2924086E-637.71428332
GGCGATA5400.037.481488
CGTTAGG2350.037.446812
GGACTAA9050.037.4364628