##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546096_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1757147
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.024672380853737	31.0	28.0	33.0	23.0	33.0
2	28.926815457101767	31.0	28.0	33.0	16.0	33.0
3	28.42169038788445	31.0	28.0	33.0	16.0	34.0
4	32.120704756061954	35.0	32.0	35.0	25.0	37.0
5	33.75461301757906	35.0	33.0	35.0	30.0	37.0
6	33.65167285377945	35.0	33.0	37.0	28.0	37.0
7	34.2541358235822	35.0	33.0	37.0	30.0	37.0
8	34.72954738561999	35.0	35.0	37.0	32.0	37.0
9	36.58320504772794	39.0	37.0	39.0	32.0	39.0
10	36.1511461477042	38.0	35.0	39.0	31.0	39.0
11	36.40009686156024	39.0	35.0	39.0	32.0	39.0
12	35.32067834962015	37.0	34.0	39.0	29.0	39.0
13	35.850731327544025	37.0	35.0	39.0	30.0	39.0
14	36.66125372549935	38.0	35.0	40.0	31.0	41.0
15	37.078950708164996	39.0	35.0	40.0	32.0	41.0
16	36.68734772901755	38.0	35.0	40.0	31.0	41.0
17	36.22947823944155	38.0	34.0	40.0	30.0	41.0
18	35.890613591236246	38.0	34.0	39.0	30.0	40.0
19	35.3144734049001	37.0	34.0	39.0	29.0	41.0
20	35.41525154127685	36.0	34.0	39.0	30.0	41.0
21	35.62172829023411	36.0	34.0	39.0	30.0	40.0
22	35.97530314765925	37.0	35.0	40.0	31.0	41.0
23	36.31943884034745	37.0	35.0	40.0	32.0	41.0
24	35.900295763530316	36.0	35.0	40.0	31.0	41.0
25	35.61518814305234	36.0	35.0	40.0	30.0	41.0
26	35.71921415795036	37.0	34.0	40.0	30.0	41.0
27	36.07299844577602	37.0	35.0	40.0	31.0	41.0
28	35.955704901183566	37.0	35.0	40.0	31.0	41.0
29	36.00052926704482	37.0	35.0	40.0	31.0	41.0
30	35.60511613427904	37.0	35.0	40.0	30.0	41.0
31	34.89817414251625	36.0	34.0	40.0	27.0	41.0
32	34.76706217521926	37.0	34.0	40.0	25.0	41.0
33	34.3086964266507	37.0	34.0	40.0	22.0	41.0
34	33.75142432591013	37.0	33.0	40.0	18.0	41.0
35	33.37935357713384	37.0	33.0	40.0	15.0	41.0
36	32.94949141989828	36.0	33.0	40.0	12.0	41.0
37	32.86741632885581	37.0	33.0	40.0	10.0	41.0
38	32.70089753446923	36.0	32.0	40.0	10.0	41.0
39	32.47913179716893	36.0	32.0	40.0	10.0	41.0
40	32.27339545297007	36.0	31.0	40.0	10.0	40.0
41	31.932331216454855	35.0	31.0	39.0	10.0	40.0
42	32.121145811932635	36.0	31.0	40.0	10.0	40.0
43	31.928979760942028	35.0	31.0	40.0	10.0	40.0
44	31.66709728895761	35.0	30.0	39.0	9.0	40.0
45	31.610317178926977	35.0	30.0	39.0	10.0	40.0
46	31.440169775209473	35.0	30.0	39.0	10.0	40.0
47	31.412514149356884	35.0	30.0	39.0	10.0	40.0
48	31.392752569932966	35.0	30.0	39.0	10.0	40.0
49	31.42560013476391	35.0	30.0	39.0	10.0	40.0
50	31.61357871595262	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	4.0
11	11.0
12	8.0
13	46.0
14	78.0
15	189.0
16	471.0
17	1088.0
18	2204.0
19	3785.0
20	5836.0
21	8567.0
22	12671.0
23	18356.0
24	27171.0
25	42238.0
26	59303.0
27	65136.0
28	58783.0
29	53386.0
30	54966.0
31	62963.0
32	80163.0
33	115341.0
34	173749.0
35	157100.0
36	173962.0
37	247160.0
38	277332.0
39	55076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.297602306466107	20.94230021734095	24.625031371877252	27.13506610431569
2	43.47894626915107	19.08417451698691	25.700923144164946	11.735956069697071
3	27.46765068602684	17.599267448881626	42.37289196635227	12.560189898739264
4	24.267007825753907	22.729230963601793	39.290850452466415	13.712910758177888
5	19.04410957079857	26.716262213690715	39.73651606837675	14.503112147133962
6	17.62510478633831	35.21395762562836	37.65569983615486	9.505237751878472
7	70.75503643121492	4.734891275459594	21.828737151757934	2.6813351415675526
8	71.92238327242968	3.776064267815954	21.337884650515864	2.9636678092384985
9	68.07495331921575	5.255792486342918	22.421800794128206	4.2474534003131215
10	33.684774239150165	26.61843317605186	28.42277851540025	11.274014069397722
11	28.715639613532616	20.638284673962964	35.65757446588134	14.988501246623077
12	26.502108247061855	18.738386714372787	40.23112465832398	14.52838038024138
13	19.70842507769697	25.83477648711235	40.16732806077124	14.289470374419441
14	15.568760041134864	30.448163984003614	39.29164719855539	14.691428776306136
15	14.742022152955899	22.945832078932497	47.376343584230575	14.93580218388103
16	16.636399800358195	18.942581354889487	47.4794652923176	16.941553552434712
17	16.361465489227708	18.7272891795621	39.4381346580565	25.4731106731537
18	18.491110874616638	22.53215012745092	41.679836689815936	17.29690230811651
19	22.11294786378146	23.586074471856936	36.938969818689046	17.362007845672558
20	22.747043929733824	21.710249626240717	36.96401041005676	18.5786960339687
21	21.160210272674966	22.96290520941048	39.14663941036237	16.730245107552186
22	18.674647027254977	20.350090231494576	37.840829480971145	23.134433260279305
23	17.185813139139754	25.50964717237659	37.508586361869554	19.795953326614107
24	17.12935798769255	23.193620112602986	43.26854839122737	16.408473508477094
25	20.271724562600625	21.6347294790931	40.06005189093457	18.03349406737171
26	16.01237688138784	25.207623494221032	37.396814267673676	21.38318535671745
27	16.149531029560986	24.581039605678978	40.55682307740901	18.71260628735103
28	15.340150824034643	23.47128612460995	44.419789579357904	16.768773471997505
29	16.658196496935087	23.754130986195236	41.618601061834894	17.96907145503478
30	17.85439692865765	24.220625821288714	41.58673121827599	16.338246031777647
31	23.81309019677921	23.34665227212066	35.999150896310894	16.841106634789234
32	22.47506896121952	24.555600641266782	35.99169562933551	16.977634768178188
33	21.09373888468068	22.83258031342853	34.86412918213445	21.209551619756343
34	17.651112855099775	24.434153773133378	35.61785098230256	22.296882389464283
35	18.218509891318142	23.905569653534965	36.5493040707465	21.326616384400396
36	24.532551915121502	24.535852720347247	32.016957033190735	18.91463833134052
37	18.55633023304254	27.63399988731734	33.06655618454233	20.743113695097794
38	19.601205818295224	29.283491933230398	31.013512244564627	20.10179000390975
39	19.41863714305064	25.496102488864054	31.587340159929706	23.497920208155605
40	20.53556133892042	23.607586616259198	35.03696617300658	20.8198858718138
41	17.932193493202334	23.496725089022146	34.780983036706665	23.79009838106886
42	20.414911216875993	26.135775777439225	30.511562208511865	22.937750797172917
43	19.685660903726323	24.589576170917972	31.342966752354812	24.381796173000893
44	19.36929579596926	25.913142156006298	33.24098666759241	21.47657538043203
45	20.155342723175693	28.37394936223321	30.263489622666746	21.207218291924352
46	22.26233775546383	26.57825440899367	30.450326580530827	20.709081255011675
47	20.949129469532146	25.741784836442257	31.525933800643884	21.783151893381714
48	18.969272348870074	27.03598503710845	32.62163040428604	21.37311220973544
49	19.332873117616227	25.95315019175971	33.383718038388366	21.3302586522357
50	18.811118250209002	26.033336994571314	34.104033413254555	21.051511341965128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1809.0
1	2322.5
2	2836.0
3	37892.0
4	72948.0
5	50343.5
6	27739.0
7	27657.0
8	27575.0
9	28359.0
10	29143.0
11	28795.0
12	28447.0
13	27369.5
14	26292.0
15	24765.0
16	23238.0
17	21736.0
18	20234.0
19	19094.5
20	17955.0
21	16734.0
22	15513.0
23	15022.5
24	14532.0
25	14541.0
26	14550.0
27	16367.0
28	18184.0
29	19519.0
30	20854.0
31	24499.5
32	28145.0
33	33644.0
34	39143.0
35	42487.5
36	45832.0
37	51306.0
38	56780.0
39	64791.5
40	72803.0
41	88824.0
42	104845.0
43	120130.5
44	135416.0
45	139520.5
46	143625.0
47	143403.0
48	143181.0
49	143443.0
50	143705.0
51	127465.5
52	111226.0
53	96725.0
54	82224.0
55	73317.0
56	64410.0
57	60369.0
58	56328.0
59	51873.0
60	47418.0
61	41581.0
62	35744.0
63	31889.5
64	28035.0
65	22760.0
66	17485.0
67	15055.5
68	12626.0
69	10961.0
70	9296.0
71	8045.0
72	6794.0
73	5800.5
74	4807.0
75	3726.5
76	2646.0
77	2039.0
78	1432.0
79	1049.0
80	666.0
81	504.5
82	343.0
83	245.5
84	148.0
85	126.5
86	105.0
87	69.0
88	33.0
89	27.5
90	22.0
91	16.0
92	10.0
93	11.0
94	12.0
95	10.5
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1757147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.68100424021469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.59641710410769	23.03139585092488
2	7.412903617289139	4.400448473941235
3	3.1080560173594196	2.7675067149020918
4	1.8629391116842162	2.21175614692644
5	1.2676756570572205	1.8812943276166152
6	0.9960993118848814	1.7739136739838062
7	0.784159999189665	1.6292259382668637
8	0.6292942726689711	1.494246878034444
9	0.5483897673282312	1.464908310842362
>10	4.851473755966251	30.854422532970737
>50	0.7768522654923149	15.861838619497703
>100	0.1565639384188732	6.794720904130609
>500	0.006051715492515545	1.325081449468359
>1k	0.0029282494318623605	1.7401218282580677
>5k	0.0	0.0
>10k+	1.9521662879082402E-4	2.7691183502357926
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47791	2.7198065955779454	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4179	0.23782870755833177	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3693	0.210170236183996	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3158	0.179723153498256	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2672	0.1520646821239202	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	2275	0.1294712394580533	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	2119	0.12059321160950108	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATG	1762	0.10027618634069888	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7073130477985053E-4	0.0	0.0	0.04740639229387183	0.0
2	1.7073130477985053E-4	0.0	0.0	0.20186131268470994	0.0
3	1.7073130477985053E-4	0.0	0.0	0.29126760595442497	0.0
4	1.7073130477985053E-4	0.0	0.0	0.45721843420043967	0.0
5	1.7073130477985053E-4	0.0	0.0	0.8788678465717439	0.0
6	1.7073130477985053E-4	0.0	0.0	1.2343873335583193	0.0
7	1.7073130477985053E-4	0.0	0.0	1.466581908058916	0.0
8	1.7073130477985053E-4	0.0	0.0	1.8921581404401566	0.0
9	1.7073130477985053E-4	0.0	0.0	2.079678023523359	0.0
10	1.7073130477985053E-4	0.0	0.0	2.5094656280891696	0.0
11	1.7073130477985053E-4	0.0	0.0	2.7986844583862363	0.0
12	1.7073130477985053E-4	0.0	0.0	3.1305292044433393	0.0
13	1.7073130477985053E-4	0.0	0.0	3.2514638786624	0.0
14	1.7073130477985053E-4	0.0	0.0	3.308032850979457	0.0
15	1.7073130477985053E-4	0.0	0.0	3.3814473120347928	0.0
16	1.7073130477985053E-4	0.0	0.0	3.503690926257166	0.0
17	1.7073130477985053E-4	0.0	0.0	3.6484141622755524	0.0
18	1.7073130477985053E-4	0.0	0.0	3.8828282437382873	0.0
19	1.7073130477985053E-4	0.0	0.0	3.986860518784143	0.0
20	2.845521746330842E-4	0.0	0.0	4.091120435569705	0.0
21	2.845521746330842E-4	0.0	0.0	4.219965660243565	0.0
22	3.4146260955970106E-4	0.0	0.0	4.363095404084007	0.0
23	3.4146260955970106E-4	0.0	0.0	4.519086906217863	0.0
24	3.4146260955970106E-4	0.0	0.0	4.646623190888412	0.0
25	3.4146260955970106E-4	0.0	0.0	4.745021332876532	0.0
26	3.4146260955970106E-4	0.0	0.0	4.842224355731194	0.0
27	3.4146260955970106E-4	0.0	0.0	4.9501834507869855	0.0
28	3.4146260955970106E-4	0.0	0.0	5.071345766745753	0.0
29	3.983730444863179E-4	0.0	0.0	5.199280424460788	0.0
30	3.983730444863179E-4	0.0	0.0	5.35265404658802	0.0
31	4.552834794129347E-4	0.0	0.0	5.479791958214082	0.0
32	4.552834794129347E-4	0.0	0.0	5.604881094182786	0.0
33	4.552834794129347E-4	0.0	0.0	5.760189671097524	0.0
34	4.552834794129347E-4	0.0	0.0	5.906904772338342	0.0
35	5.121939143395515E-4	0.0	0.0	6.092717342373746	0.0
36	5.121939143395515E-4	0.0	0.0	6.227367431410121	0.0
37	5.691043492661684E-4	0.0	0.0	6.363497191754589	0.0
38	5.691043492661684E-4	0.0	0.0	6.521651290415657	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGT	20	7.85762E-4	44.0	30
ATCGACG	25	4.4438035E-5	44.0	15
CGTTTTT	29910	0.0	43.418922	1
TTGCACG	135	0.0	40.74074	1
CGGTCTA	250	0.0	40.48	31
GTTTTTT	33345	0.0	39.691708	2
GTTGATC	1315	0.0	39.31559	16
CGACGGT	280	0.0	38.499996	28
CACGACG	270	0.0	38.296295	26
TGCACGG	270	0.0	38.296295	2
AGGGTAC	910	0.0	38.197803	6
ACGATAG	75	0.0	38.13333	1
TCGTCCC	400	0.0	37.95	38
GCCAGTA	1345	0.0	37.947956	25
TGCGTAG	105	0.0	37.714287	1
CCATACG	35	7.2924086E-6	37.714283	34
CCCGATT	35	7.2924086E-6	37.714283	32
GGCGATA	540	0.0	37.48148	8
CGTTAGG	235	0.0	37.44681	2
GGACTAA	905	0.0	37.436462	8
>>END_MODULE