FastQCFastQC Report
Thu 26 May 2016
SRR1546095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546095_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1731195
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480212.7738642960498385No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT41550.24000762479096807No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT36770.21239663931561728No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31700.18311051037000453No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT26710.15428648996791233No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC23370.1349934582759308No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT20830.12032151201915439No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC254.4437773E-544.034
GTCGTAC254.4437773E-544.09
TGCCGGT254.4437773E-544.022
CGTTTTT297250.043.3709031
TCGATAG850.041.4117661
ATAGCGG2850.040.9122772
TATTCGT650.040.61538744
GCCCGAA3050.040.3934425
CGGGATA2950.040.2711876
GTTTTTT330000.039.6333352
TACGGGA2950.039.525424
GATCGAC452.3521352E-839.111119
TATTACG452.3521352E-839.111111
TAGACGG1300.038.923082
GGCGATA5100.038.823538
AGGGTAC8550.038.5964936
ACGGGCT2950.038.0338975
TTCGTAG1100.038.01
GACGGGA6850.037.897814
TCGCCGT357.292343E-637.71428725
Produced by FastQC (version 0.11.5)