FastQCFastQC Report
Thu 26 May 2016
SRR1546094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546094_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1525940
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246931.6182156572342294No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT26300.17235277927048245No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT23050.15105443202222893No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT18210.11933627796636827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC254.4435288E-544.011
CGTTTTT275050.043.2881281
CGGTCTA2050.039.70731731
CGGAATT3550.039.6619729
TACGTAG1000.039.61
GTTTTTT305050.039.5718732
GCGGAAT3800.039.368428
ACGGGAT4850.039.0103075
AGGGTAC6950.038.935256
TACCGGT404.1257226E-738.525
CGCAAGA7950.037.9119520
ATAGCGG2150.037.8604662
GGCGTAT357.2917774E-637.7142877
TCGAACG357.2917774E-637.7142871
GTCGGTA357.2917774E-637.71428718
GTAGCAT12200.037.68852629
GGGTACC11800.037.474587
GGTACCT11350.037.4096958
GTTGATC12500.037.31216
ACGGTCT2300.037.30434830
Produced by FastQC (version 0.11.5)