##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546094_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1525940
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.955939289880337	28.0	25.0	30.0	16.0	33.0
2	26.28669213730553	30.0	25.0	31.0	16.0	33.0
3	26.629185944401485	30.0	25.0	31.0	16.0	33.0
4	27.766651375545564	32.0	19.0	35.0	10.0	35.0
5	31.32977115745049	33.0	30.0	35.0	19.0	37.0
6	30.02496428431	33.0	28.0	35.0	17.0	36.0
7	30.080114552341506	32.0	28.0	35.0	17.0	35.0
8	32.483616655962884	35.0	32.0	35.0	28.0	35.0
9	27.264801368336894	31.0	23.0	34.0	10.0	37.0
10	31.47112992647155	34.0	28.0	37.0	17.0	39.0
11	33.727934256917045	35.0	32.0	37.0	27.0	39.0
12	33.380981558907955	35.0	32.0	39.0	23.0	39.0
13	33.89639894098064	35.0	32.0	39.0	25.0	39.0
14	33.74565644782888	36.0	31.0	39.0	24.0	40.0
15	32.32024194922474	35.0	28.0	38.0	18.0	40.0
16	30.863456623458326	34.0	27.0	38.0	17.0	40.0
17	31.05887256379674	33.0	27.0	38.0	17.0	40.0
18	32.03433228043043	34.0	29.0	38.0	18.0	39.0
19	31.714655884241843	35.0	29.0	37.0	18.0	39.0
20	31.996682045165603	34.0	30.0	37.0	19.0	39.0
21	33.03115915435731	35.0	31.0	37.0	25.0	39.0
22	33.3540171959579	35.0	31.0	37.0	26.0	39.0
23	34.52132521593247	35.0	33.0	38.0	29.0	40.0
24	34.12254348139508	35.0	33.0	38.0	27.0	40.0
25	33.030571975307026	35.0	31.0	38.0	23.0	40.0
26	26.51024876469586	33.0	20.0	36.0	0.0	39.0
27	29.942338492994484	34.0	24.0	37.0	15.0	39.0
28	31.425172680446153	34.0	29.0	37.0	20.0	39.0
29	32.56535512536535	35.0	30.0	37.0	23.0	39.0
30	32.367703186232745	35.0	30.0	38.0	22.0	39.0
31	31.21672804959566	34.0	29.0	37.0	18.0	39.0
32	31.461781590363973	34.0	30.0	37.0	18.0	39.0
33	31.016125797868856	34.0	29.0	37.0	16.0	39.0
34	30.74999606799743	34.0	29.0	38.0	13.0	39.0
35	29.755391430855735	34.0	26.0	37.0	9.0	39.0
36	29.56661795352373	34.0	25.0	37.0	9.0	39.0
37	29.730942894216025	34.0	25.0	38.0	9.0	39.0
38	29.42332726057381	34.0	25.0	37.0	9.0	39.0
39	29.375839810215343	34.0	25.0	37.0	8.0	39.0
40	29.18625634035414	33.0	24.0	37.0	8.0	39.0
41	28.48957363985478	33.0	23.0	36.0	8.0	38.0
42	28.729240337103686	33.0	23.0	37.0	8.0	38.0
43	28.34922736149521	33.0	23.0	36.0	8.0	38.0
44	28.110635411615135	33.0	23.0	36.0	7.0	38.0
45	27.515160491238188	31.0	23.0	35.0	8.0	38.0
46	27.083144160320852	31.0	21.0	35.0	7.0	38.0
47	27.07835498119192	31.0	22.0	35.0	7.0	38.0
48	27.103342857517333	31.0	22.0	35.0	7.0	38.0
49	26.941452481748954	31.0	20.0	35.0	7.0	38.0
50	28.756142443346395	33.0	23.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	11.0
11	32.0
12	57.0
13	168.0
14	454.0
15	1049.0
16	2321.0
17	4197.0
18	7118.0
19	11539.0
20	17122.0
21	24323.0
22	33027.0
23	43406.0
24	56555.0
25	69191.0
26	79326.0
27	83688.0
28	88729.0
29	100266.0
30	118091.0
31	138418.0
32	155232.0
33	161841.0
34	149262.0
35	113600.0
36	55665.0
37	10981.0
38	267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.551659960417837	21.670773424905303	24.306067079963825	28.47149953471303
2	43.834292305070974	16.464081156533023	27.091890900035388	12.609735638360617
3	28.54640418365073	13.734091772939957	44.170478524712635	13.549025518696672
4	25.388678453936592	22.42355531672281	38.49705755141093	13.690708677929669
5	18.282697878029282	26.772743358192326	40.710185197320996	14.234373566457398
6	17.516743777605935	33.832981637548	38.969946393698315	9.680328191147751
7	72.66747054274742	3.34790358729701	22.08533756242054	1.8992883075350278
8	74.49080566732637	2.408613706960955	21.13431720775391	1.9662634179587664
9	62.42768391942016	3.433752310051509	20.560769099702476	13.577794670825853
10	34.02709149769978	27.02786479153833	28.86358572420934	10.081457986552552
11	29.054091248672947	20.273339711915277	36.295463779703	14.37710525970877
12	26.57928883180204	18.68638347510387	41.25168748443582	13.48264020865827
13	19.01588529037839	26.20260298570062	40.90259118969291	13.878920534228081
14	14.819914282344	30.601203192786087	40.24614336081366	14.332739164056255
15	13.320117435810058	21.370499495393002	47.15021560480753	18.15916746398941
16	14.85707170662018	16.94024666762782	47.48063488734813	20.72204673840387
17	15.620338938621439	17.828289447815774	40.33972502195368	26.211646591609107
18	17.876653079413344	22.22000865040565	42.807253234072114	17.096085036108892
19	22.275187753122665	23.041797187307495	37.82212931045781	16.860885749112022
20	22.872393409963696	21.336946406804984	37.86177700302764	17.928883180203677
21	20.92710067237244	22.31968491552748	40.130542485287755	16.622671926812327
22	18.24154291780804	19.878828787501472	38.541423647063446	23.338204647627038
23	16.691744105272814	25.510242866692007	38.2732610718639	19.524751956171276
24	16.439571673853493	22.764787606327904	45.006291204110255	15.78934951570835
25	19.932304022438625	20.413384536744562	41.608254584059665	18.046056856757147
26	12.757775535080015	37.47146021468734	31.333800804749863	18.436963445482785
27	15.30551659960418	25.03263562132194	41.629683998060216	18.032163781013672
28	14.620496218724197	23.148682123805656	45.97559537072231	16.25522628674784
29	15.87893364090331	23.4941740828604	42.96512313721379	17.661769139022503
30	17.330825589472717	23.576877203559775	42.94428352359857	16.14801368336894
31	24.239157502916235	22.804042098640835	36.68342136650196	16.27337903194097
32	22.333053724261767	24.078010930967142	36.59193677339869	16.996998571372398
33	20.825982672975346	22.300811303196717	35.64884595724603	21.22436006658191
34	17.07164108680551	23.944060710119665	35.91340419675741	23.070894006317417
35	17.909616367616028	23.452756989134567	36.57824029778366	22.059386345465747
36	24.4638059163532	23.93908017353238	32.04280640130018	19.55430750881424
37	17.884320484422716	27.751353264217464	32.765967207098576	21.598359044261244
38	19.029909432874163	29.708114342634705	30.875001638334403	20.386974586156732
39	18.815287625987914	25.29555552642961	31.618477790738826	24.27067905684365
40	20.188736123307603	23.117815903639723	35.25938110279566	21.43406687025702
41	17.30074576982057	22.742964992070462	35.112586340223075	24.843702897885894
42	19.73799756215841	25.64406202078719	29.787671861278948	24.830268555775454
43	19.09727774355479	24.05396018192065	31.43026593444041	25.418496140084144
44	18.692347012333382	25.39005465483571	33.47359660274978	22.44400173008113
45	19.708900743148487	29.029778366121867	29.599001271347497	21.66231961938215
46	21.501566247689947	26.061181959972213	30.27419164580521	22.16306014653263
47	20.579315045152498	25.164947507765707	30.903836323839734	23.351901123242065
48	18.192458419072835	26.661074485235332	32.81111970326487	22.335347392426964
49	18.66790306303	26.003250455456968	32.73667378796021	22.592172693552826
50	18.326277573168014	26.211122324599916	34.154947114565445	21.30765298766662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1086.0
1	1462.0
2	1838.0
3	22387.5
4	42937.0
5	33754.0
6	24571.0
7	24167.5
8	23764.0
9	25068.0
10	26372.0
11	26907.5
12	27443.0
13	27255.0
14	27067.0
15	26217.5
16	25368.0
17	24108.0
18	22848.0
19	21844.5
20	20841.0
21	19478.0
22	18115.0
23	17246.0
24	16377.0
25	16136.5
26	15896.0
27	16914.0
28	17932.0
29	19254.0
30	20576.0
31	23448.5
32	26321.0
33	30597.0
34	34873.0
35	37424.5
36	39976.0
37	44413.0
38	48850.0
39	55142.5
40	61435.0
41	74203.0
42	86971.0
43	97516.5
44	108062.0
45	113565.5
46	119069.0
47	119931.0
48	120793.0
49	120958.0
50	121123.0
51	108517.0
52	95911.0
53	83851.5
54	71792.0
55	64316.5
56	56841.0
57	53123.0
58	49405.0
59	45701.5
60	41998.0
61	36872.0
62	31746.0
63	28338.0
64	24930.0
65	20646.5
66	16363.0
67	14020.0
68	11677.0
69	10173.5
70	8670.0
71	7507.5
72	6345.0
73	5448.0
74	4551.0
75	3497.5
76	2444.0
77	1885.5
78	1327.0
79	997.0
80	667.0
81	512.5
82	358.0
83	280.0
84	202.0
85	151.0
86	100.0
87	75.5
88	51.0
89	41.5
90	32.0
91	22.0
92	12.0
93	8.0
94	4.0
95	6.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1525940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.91091355716083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32362809436083	36.97229345057696
2	7.216038611110395	6.481577725774276
3	2.749909787019296	3.7050288220444245
4	1.533298285935804	2.7544730710802305
5	1.0042997736951256	2.2552010160948983
6	0.7145711758547518	1.925522658555092
7	0.5166378145707374	1.624187336138281
8	0.4145027766503042	1.4892558697075964
9	0.33961113127327625	1.372702154369734
>10	2.864886338751388	26.524052571941308
>50	0.27459367888240194	8.220973331976376
>100	0.044047164170776455	3.3912150298251516
>500	0.0031802941799063214	0.9627450555392717
>1k	6.360588359812642E-4	0.5573223361344468
>5k	0.0	0.0
>10k+	1.5901470899531606E-4	1.7634495702419137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24693	1.6182156572342294	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2630	0.17235277927048245	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2305	0.15105443202222893	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1821	0.11933627796636827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0368297573954415	0.0
2	0.0	0.0	0.0	0.15007143138000184	0.0
3	0.0	0.0	0.0	0.2342818197307889	0.0
4	0.0	0.0	0.0	0.3228829442835236	0.0
5	0.0	0.0	0.0	0.5836402479782954	0.0
6	0.0	0.0	0.0	0.7790607756530401	0.0
7	0.0	0.0	0.0	0.8988557872524476	0.0
8	0.0	0.0	0.0	1.136807476047551	0.0
9	0.0	0.0	0.0	1.2778353015190635	0.0
10	0.0	0.0	0.0	1.5457357432140189	0.0
11	0.0	0.0	0.0	1.748561542393541	0.0
12	0.0	0.0	0.0	1.9599066804723646	0.0
13	6.553337614847242E-5	0.0	0.0	2.0426098011717366	0.0
14	6.553337614847242E-5	0.0	0.0	2.0781288910442086	0.0
15	6.553337614847242E-5	0.0	0.0	2.124329921228882	0.0
16	6.553337614847242E-5	0.0	0.0	2.1964166349922016	0.0
17	6.553337614847242E-5	0.0	0.0	2.3092651087198712	0.0
18	6.553337614847242E-5	0.0	0.0	2.451996801971244	0.0
19	6.553337614847242E-5	0.0	0.0	2.5231005150923367	0.0
20	6.553337614847242E-5	0.0	0.0	2.588502824488512	0.0
21	6.553337614847242E-5	0.0	0.0	2.6669462757382334	0.0
22	6.553337614847242E-5	0.0	0.0	2.75462993302489	0.0
23	6.553337614847242E-5	0.0	0.0	2.844869391981336	0.0
24	6.553337614847242E-5	0.0	0.0	2.914072637194123	0.0
25	6.553337614847242E-5	0.0	0.0	2.966499338112901	0.0
26	6.553337614847242E-5	0.0	0.0	3.0258070435272684	0.0
27	6.553337614847242E-5	0.0	0.0	3.0993354915658546	0.0
28	6.553337614847242E-5	0.0	0.0	3.1886574832562222	0.0
29	6.553337614847242E-5	0.0	0.0	3.2842706790568434	0.0
30	6.553337614847242E-5	0.0	0.0	3.396005085389989	0.0
31	6.553337614847242E-5	0.0	0.0	3.492797881961283	0.0
32	6.553337614847242E-5	0.0	0.0	3.585199942330629	0.0
33	6.553337614847242E-5	0.0	0.0	3.7012595514895734	0.0
34	6.553337614847242E-5	0.0	0.0	3.8136492915842037	0.0
35	6.553337614847242E-5	0.0	0.0	3.9646381902302843	0.0
36	6.553337614847242E-5	0.0	0.0	4.066542590141159	0.0
37	6.553337614847242E-5	0.0	0.0	4.167660589538252	0.0
38	6.553337614847242E-5	0.0	0.0	4.2903390696881925	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	25	4.4435288E-5	44.0	11
CGTTTTT	27505	0.0	43.288128	1
CGGTCTA	205	0.0	39.707317	31
CGGAATT	355	0.0	39.661972	9
TACGTAG	100	0.0	39.6	1
GTTTTTT	30505	0.0	39.571873	2
GCGGAAT	380	0.0	39.36842	8
ACGGGAT	485	0.0	39.010307	5
AGGGTAC	695	0.0	38.93525	6
TACCGGT	40	4.1257226E-7	38.5	25
CGCAAGA	795	0.0	37.91195	20
ATAGCGG	215	0.0	37.860466	2
GGCGTAT	35	7.2917774E-6	37.714287	7
TCGAACG	35	7.2917774E-6	37.714287	1
GTCGGTA	35	7.2917774E-6	37.714287	18
GTAGCAT	1220	0.0	37.688526	29
GGGTACC	1180	0.0	37.47458	7
GGTACCT	1135	0.0	37.409695	8
GTTGATC	1250	0.0	37.312	16
ACGGTCT	230	0.0	37.304348	30
>>END_MODULE