FastQCFastQC Report
Thu 26 May 2016
SRR1546093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546093_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1479433
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355912.405718947732003No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31070.21001289007342677No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21050.14228423997572043No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20700.13991846876472272No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT19950.13484895902687044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC351.4464604E-744.01
CTATCGA207.857215E-444.010
CGCATCG408.314601E-944.021
CGTCTAG207.857215E-444.032
CTAAGCG254.443462E-544.01
AGACCGG408.314601E-944.02
TACTACG207.857215E-444.01
GACGATA254.443462E-544.011
CACCGTA207.857215E-444.025
ATCGAGT207.857215E-444.011
TACCGAA351.4464604E-744.027
CGTTTTT219000.043.236531
CGACGGT2800.042.42857428
CGGTCTA2750.042.431
AGTACGG1500.041.0666662
CTATACG650.040.6153871
TAGCTGA1300.040.6153876
CGGGATA2550.040.5490156
ATTCGGC603.6379788E-1240.33333217
TCGATAG557.8216544E-1140.01
Produced by FastQC (version 0.11.5)