##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546093_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1479433
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.032761875664526	31.0	28.0	33.0	23.0	33.0
2	28.89289680573571	31.0	28.0	33.0	16.0	33.0
3	28.324423613641173	31.0	28.0	33.0	16.0	34.0
4	32.145874804739385	35.0	32.0	35.0	25.0	37.0
5	33.66500950026125	35.0	33.0	35.0	28.0	37.0
6	33.474758911015236	35.0	33.0	37.0	28.0	37.0
7	34.25516126786411	35.0	33.0	37.0	30.0	37.0
8	34.73075968969193	35.0	35.0	37.0	32.0	37.0
9	36.51898734177215	39.0	37.0	39.0	32.0	39.0
10	36.308640539990655	38.0	35.0	39.0	32.0	39.0
11	36.44435401941149	39.0	35.0	39.0	32.0	39.0
12	35.1852885531146	37.0	34.0	39.0	30.0	39.0
13	35.158773665316374	37.0	34.0	39.0	27.0	39.0
14	35.92060133848576	38.0	34.0	40.0	27.0	41.0
15	36.543118208124326	38.0	35.0	40.0	31.0	41.0
16	36.217305548814984	38.0	34.0	40.0	31.0	41.0
17	35.70924401442985	37.0	34.0	40.0	29.0	41.0
18	35.37263870685594	37.0	34.0	39.0	29.0	40.0
19	34.862423644734164	37.0	33.0	39.0	27.0	40.0
20	35.08282902977019	35.0	34.0	39.0	29.0	40.0
21	35.30364673493156	35.0	34.0	39.0	30.0	40.0
22	35.62376667277261	35.0	34.0	39.0	31.0	40.0
23	35.98588783675908	36.0	35.0	40.0	31.0	41.0
24	35.67304298335917	35.0	34.0	40.0	31.0	41.0
25	35.35229577817989	35.0	34.0	40.0	30.0	41.0
26	35.43506059416006	35.0	34.0	40.0	30.0	41.0
27	35.712933265649745	36.0	34.0	40.0	31.0	41.0
28	35.6436350953372	36.0	35.0	40.0	31.0	41.0
29	35.7701105761464	36.0	35.0	40.0	31.0	41.0
30	35.392645020085396	36.0	34.0	40.0	30.0	41.0
31	34.68078851830397	35.0	34.0	39.0	28.0	41.0
32	34.51287418896293	35.0	34.0	40.0	25.0	41.0
33	34.09839648027319	35.0	33.0	40.0	23.0	41.0
34	33.69707448732048	35.0	33.0	40.0	20.0	41.0
35	33.38557474383767	35.0	33.0	40.0	18.0	41.0
36	32.96758487880154	35.0	33.0	40.0	15.0	41.0
37	32.89069528664022	35.0	33.0	40.0	15.0	41.0
38	32.74086896804384	35.0	32.0	40.0	15.0	41.0
39	32.60268359567483	35.0	32.0	40.0	15.0	40.0
40	32.386635961209464	35.0	32.0	39.0	12.0	40.0
41	32.11810808600322	35.0	31.0	39.0	10.0	40.0
42	32.293151497904944	35.0	32.0	39.0	10.0	40.0
43	32.07324630449639	35.0	31.0	39.0	10.0	40.0
44	31.867777047017338	35.0	31.0	39.0	10.0	40.0
45	31.774301370863025	35.0	31.0	39.0	10.0	40.0
46	31.634094953945194	35.0	31.0	38.0	10.0	40.0
47	31.598119009106867	35.0	31.0	38.0	10.0	40.0
48	31.615636531022357	35.0	31.0	38.0	10.0	40.0
49	31.63062335367671	35.0	31.0	38.0	10.0	40.0
50	31.74063306685737	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	5.0
12	10.0
13	15.0
14	53.0
15	158.0
16	377.0
17	936.0
18	1757.0
19	3148.0
20	4946.0
21	7221.0
22	10190.0
23	14622.0
24	21147.0
25	32241.0
26	45697.0
27	51714.0
28	48352.0
29	45980.0
30	49077.0
31	58563.0
32	77261.0
33	118650.0
34	189345.0
35	137301.0
36	131096.0
37	181643.0
38	203342.0
39	44584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.053202138927553	20.9195685103685	26.928965353618583	25.09826399708537
2	40.32524622608797	18.966860952810976	28.587708939843846	12.120183881257212
3	28.075823643247112	17.882932177394988	41.47690365160166	12.564340527756244
4	25.148485940221693	23.955326128320785	37.204320844539765	13.691867086917759
5	19.533834921892375	27.98970957116679	38.548754827018186	13.927700679922648
6	17.519008971680368	35.82798274744446	36.77827924617066	9.874729034704512
7	73.65206805580246	4.942163653237422	19.04249803809973	2.3632702528603864
8	75.1362177266561	3.2937618668773783	18.615239757393542	2.9547806490729895
9	71.55667069749019	4.745399081945583	20.188410019243857	3.509520201320371
10	42.21441592826441	22.688083880784056	24.60206038394439	10.495439807007143
11	38.77086694699929	18.18135731729656	29.973307341393628	13.074468394310523
12	34.80272509806122	18.24827484583621	34.57520550102641	12.373794555076167
13	19.631439882711824	32.727335404847665	35.04923845824718	12.59198625419333
14	14.150691514924974	36.19569118709668	36.758136394145595	12.895480903832752
15	12.696620935182601	21.28937234737903	52.76906760900967	13.244939108428703
16	15.032786209311269	16.205330014944916	52.39284239299786	16.36904138274596
17	15.321815857832021	16.346600352973066	37.068931137807525	31.26265265138739
18	19.192961087119187	21.423950932553215	41.66373198380731	17.71935599652029
19	24.460519672063555	22.4675264104559	35.5465235667989	17.525430350681646
20	26.19125029656632	20.61073397713854	33.48444978583011	19.71356594046503
21	20.460270928119083	24.39238546118682	37.23696848725154	17.910375123442563
22	19.038036869530423	20.565851917592752	35.11561523908146	25.280495973795365
23	16.52869714275672	26.575383947769176	34.678691093141765	22.21722781633234
24	17.542396309937658	21.76320252421029	45.235843732024364	15.458557433827687
25	17.455335929372943	20.33170816116715	42.59239857431867	19.620557335141235
26	14.50298864497412	27.712305998311514	36.78247004088729	21.00223531582708
27	15.111600187369078	27.955507278802084	38.76559465687192	18.167297876956916
28	13.746685385549734	24.7254860476953	45.28762032481362	16.24020824194134
29	14.87705087016445	23.304468671443722	42.55812868849079	19.260351769901035
30	17.310145170480855	25.06264224199406	40.68335639396985	16.943856193555234
31	26.510899783903696	22.94649369048818	33.32337456309275	17.21923196251537
32	25.12313839153243	24.4731596496766	34.70924333849522	15.694458620295748
33	24.047793985939208	23.883001122727425	32.331508084516166	19.737696806817205
34	17.474059318671408	25.660574017207942	33.39468566673854	23.470680997382104
35	17.380645152568587	24.070099828785757	35.46162617705567	23.08762884158999
36	27.601858279489505	23.910782036090854	30.54548600713922	17.94187367728042
37	18.35987165353213	30.288428066698525	31.606230224687433	19.745470055081913
38	19.130910287927875	31.34356202680351	28.32666298507604	21.198864700192573
39	19.424130731165253	27.556029911459323	30.570292808123114	22.449546549252315
40	21.73062247496169	23.209094294908926	32.043357151016636	23.01692607911274
41	16.42311615328305	23.123994124776182	33.05989524365078	27.392994478289996
42	20.51671146986717	25.019787986343417	28.85815038599247	25.60535015779694
43	20.441885506136472	23.368006526824804	30.26693334541003	25.923174621628693
44	18.568194707026272	26.903955772245176	32.89550794121802	21.632341579510527
45	18.092877474005245	32.451216107792646	27.85878103300386	21.59712538519825
46	22.15044547471903	28.196410381544823	29.77221678845882	19.880927355277326
47	20.72469655604546	25.98840231358906	30.146279013649146	23.140622116716337
48	20.248230234150515	24.915626459596346	33.119512678167915	21.716630628085216
49	20.141500155802934	24.039277209579616	34.09813083796292	21.72109179665453
50	18.10058312880678	28.52504979948399	32.65284740843283	20.721519663276403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	790.0
1	1404.5
2	2019.0
3	27394.5
4	52770.0
5	36308.0
6	19846.0
7	20502.0
8	21158.0
9	22085.5
10	23013.0
11	22835.5
12	22658.0
13	21383.0
14	20108.0
15	18620.0
16	17132.0
17	15932.5
18	14733.0
19	13764.0
20	12795.0
21	12108.0
22	11421.0
23	11068.0
24	10715.0
25	10756.0
26	10797.0
27	11034.5
28	11272.0
29	14253.5
30	17235.0
31	18128.0
32	19021.0
33	22071.0
34	25121.0
35	28028.5
36	30936.0
37	33648.0
38	36360.0
39	46171.0
40	55982.0
41	78231.0
42	100480.0
43	121841.5
44	143203.0
45	143847.5
46	144492.0
47	136054.5
48	127617.0
49	122294.0
50	116971.0
51	106363.0
52	95755.0
53	84351.5
54	72948.0
55	67254.5
56	61561.0
57	55468.5
58	49376.0
59	44095.0
60	38814.0
61	34411.5
62	30009.0
63	25985.5
64	21962.0
65	18274.5
66	14587.0
67	12244.0
68	9901.0
69	8098.5
70	6296.0
71	5270.0
72	4244.0
73	3348.0
74	2452.0
75	1901.0
76	1350.0
77	1046.5
78	743.0
79	594.0
80	445.0
81	311.5
82	178.0
83	131.0
84	84.0
85	60.5
86	37.0
87	26.0
88	15.0
89	13.5
90	12.0
91	12.0
92	12.0
93	8.0
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1479433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.139232997957052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.444754177292	21.56069694082949
2	7.35897700224764	3.9943398298121235
3	2.8979433475678085	2.359438791735671
4	1.693265359135247	1.8381569243576357
5	1.143277479652498	1.5513836950803124
6	0.8345647817794875	1.3589668838761615
7	0.6582455353681034	1.2504995267953958
8	0.5176053137082359	1.1237928967766773
9	0.4251855545494411	1.0385288849054575
>10	3.5884134768481757	21.247045674604212
>50	0.8886536599848138	17.102875950780028
>100	0.5411489184125211	21.315362805294527
>500	0.006471882181537081	1.1446448716874158
>1k	0.0012445927272186695	0.7099311234987636
>5k	0.0	0.0
>10k+	2.489185454437339E-4	2.4043351999661717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35591	2.405718947732003	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3107	0.21001289007342677	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2105	0.14228423997572043	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2070	0.13991846876472272	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1995	0.13484895902687044	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3518692634272725E-4	0.0	0.0	0.049208041188752716	0.0
2	1.3518692634272725E-4	0.0	0.0	0.20433503916703225	0.0
3	2.0278038951409088E-4	0.0	0.0	0.2781471009501613	0.0
4	2.0278038951409088E-4	0.0	0.0	0.4094136064289495	0.0
5	2.0278038951409088E-4	0.0	0.0	0.7159499619110835	0.0
6	2.0278038951409088E-4	0.0	0.0	1.0078185358850316	0.0
7	2.703738526854545E-4	0.0	0.0	1.2144517527998901	0.0
8	2.703738526854545E-4	0.0	0.0	1.765946818815046	0.0
9	2.703738526854545E-4	0.0	0.0	1.9965081216925673	0.0
10	2.703738526854545E-4	0.0	0.0	2.380438992505913	0.0
11	2.703738526854545E-4	0.0	0.0	2.687989249935617	0.0
12	3.3796731585681813E-4	0.0	0.0	2.9661363508857783	0.0
13	3.3796731585681813E-4	0.0	0.0	3.0810452382770968	0.0
14	3.3796731585681813E-4	0.0	0.0	3.1217365031062574	0.0
15	3.3796731585681813E-4	0.0	0.0	3.1841928630765977	0.0
16	3.3796731585681813E-4	0.0	0.0	3.3330336689799402	0.0
17	3.3796731585681813E-4	0.0	0.0	3.517699010364106	0.0
18	4.0556077902818177E-4	0.0	0.0	3.8284937540260358	0.0
19	4.7315424219954535E-4	0.0	0.0	3.930019135709424	0.0
20	5.40747705370909E-4	0.0	0.0	4.055607790281817	0.0
21	5.40747705370909E-4	0.0	0.0	4.204854156964188	0.0
22	5.40747705370909E-4	0.0	0.0	4.348355079276994	0.0
23	5.40747705370909E-4	0.0	0.0	4.5209211907534845	0.0
24	5.40747705370909E-4	0.0	0.0	4.644346854504395	0.0
25	5.40747705370909E-4	0.0	0.0	4.741208287228958	0.0
26	5.40747705370909E-4	0.0	0.0	4.850033762934854	0.0
27	6.083411685422726E-4	0.0	0.0	4.965483398031544	0.0
28	6.759346317136363E-4	0.0	0.0	5.086205323255599	0.0
29	6.759346317136363E-4	0.0	0.0	5.210847669343593	0.0
30	6.759346317136363E-4	0.0	0.0	5.365907073858701	0.0
31	7.435280948849998E-4	0.0	0.0	5.505149607991711	0.0
32	7.435280948849998E-4	0.0	0.0	5.630467888711419	0.0
33	7.435280948849998E-4	0.0	0.0	5.7711298855710265	0.0
34	8.111215580563635E-4	0.0	0.0	5.910980760872578	0.0
35	8.111215580563635E-4	0.0	0.0	6.101999887794851	0.0
36	8.111215580563635E-4	0.0	0.0	6.241039641538346	0.0
37	8.111215580563635E-4	0.0	0.0	6.383797035756266	0.0
38	8.111215580563635E-4	0.0	0.0	6.528582233869327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	35	1.4464604E-7	44.0	1
CTATCGA	20	7.857215E-4	44.0	10
CGCATCG	40	8.314601E-9	44.0	21
CGTCTAG	20	7.857215E-4	44.0	32
CTAAGCG	25	4.443462E-5	44.0	1
AGACCGG	40	8.314601E-9	44.0	2
TACTACG	20	7.857215E-4	44.0	1
GACGATA	25	4.443462E-5	44.0	11
CACCGTA	20	7.857215E-4	44.0	25
ATCGAGT	20	7.857215E-4	44.0	11
TACCGAA	35	1.4464604E-7	44.0	27
CGTTTTT	21900	0.0	43.23653	1
CGACGGT	280	0.0	42.428574	28
CGGTCTA	275	0.0	42.4	31
AGTACGG	150	0.0	41.066666	2
CTATACG	65	0.0	40.615387	1
TAGCTGA	130	0.0	40.615387	6
CGGGATA	255	0.0	40.549015	6
ATTCGGC	60	3.6379788E-12	40.333332	17
TCGATAG	55	7.8216544E-11	40.0	1
>>END_MODULE