FastQCFastQC Report
Thu 26 May 2016
SRR1546092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546092_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1480196
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362952.4520401352253347No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31040.2097019583892944No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21520.14538615156371185No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT21410.14464300673694563No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20300.13714399984866868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC207.857217E-444.042
CTATCGA254.4434644E-544.010
CCGGAAC207.857217E-444.027
TCGATAG800.044.01
TAGCCGT408.314601E-944.044
TACGCTA254.4434644E-544.036
CCGTAAT254.4434644E-544.014
CGGATTA207.857217E-444.019
GCGATAC351.4464604E-744.09
GACGATA351.4464604E-744.011
TATCGCG254.4434644E-544.01
TAGTCCG254.4434644E-544.01
TACGTTC207.857217E-444.031
GTCGGTA302.5282134E-644.010
TAGTACG700.044.01
CGTTTTT215500.043.4385151
CGACGGT2250.042.04444528
CGGTCTA2250.042.04444531
AGTACGG950.041.684212
ATTCGGC700.040.85714317
Produced by FastQC (version 0.11.5)