##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546092_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1480196
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.000030401379277	31.0	28.0	33.0	23.0	33.0
2	28.861087315463628	31.0	28.0	33.0	16.0	33.0
3	28.309438074417173	31.0	28.0	33.0	16.0	34.0
4	32.12399776786317	35.0	32.0	35.0	25.0	37.0
5	33.695744347370216	35.0	33.0	35.0	28.0	37.0
6	33.54988055635875	35.0	33.0	37.0	28.0	37.0
7	34.31246470062073	35.0	35.0	37.0	31.0	37.0
8	34.76386505570884	35.0	35.0	37.0	32.0	37.0
9	36.62712978551489	39.0	37.0	39.0	32.0	39.0
10	36.31522852379009	38.0	35.0	39.0	32.0	39.0
11	36.498493442760285	39.0	35.0	39.0	32.0	39.0
12	35.15310742631381	37.0	34.0	39.0	29.0	39.0
13	35.18753935289651	37.0	34.0	39.0	27.0	39.0
14	35.95034576502031	38.0	34.0	40.0	27.0	41.0
15	36.595393447894736	38.0	35.0	40.0	31.0	41.0
16	36.262041648538435	38.0	34.0	40.0	31.0	41.0
17	35.7676388802564	37.0	34.0	40.0	30.0	41.0
18	35.46216649686934	37.0	34.0	39.0	29.0	40.0
19	34.93853111344714	37.0	33.0	39.0	27.0	40.0
20	35.076843877432445	35.0	34.0	39.0	29.0	40.0
21	35.228822399195785	35.0	34.0	39.0	30.0	40.0
22	35.612123664703866	35.0	34.0	39.0	31.0	40.0
23	36.01625798205102	36.0	35.0	40.0	31.0	41.0
24	35.586349375352995	35.0	34.0	40.0	31.0	41.0
25	35.276212069212455	35.0	34.0	39.0	30.0	41.0
26	35.40273652948664	35.0	34.0	40.0	30.0	41.0
27	35.7308565892625	36.0	34.0	40.0	31.0	41.0
28	35.65120835348832	36.0	35.0	40.0	31.0	41.0
29	35.784180608514006	36.0	35.0	40.0	31.0	41.0
30	35.440834186823906	36.0	35.0	40.0	30.0	41.0
31	34.71826231120743	35.0	34.0	39.0	28.0	41.0
32	34.59404227548244	35.0	34.0	40.0	26.0	41.0
33	34.19002956365238	35.0	34.0	40.0	23.0	41.0
34	33.71611124472705	35.0	33.0	40.0	20.0	41.0
35	33.42588954435764	35.0	33.0	40.0	18.0	41.0
36	32.99532764579826	35.0	33.0	40.0	15.0	41.0
37	32.9198997970539	35.0	33.0	40.0	15.0	41.0
38	32.802914614010575	35.0	33.0	40.0	15.0	41.0
39	32.6290639888231	35.0	32.0	40.0	15.0	40.0
40	32.39541047266713	35.0	32.0	39.0	12.0	40.0
41	32.120663074349615	35.0	31.0	39.0	10.0	40.0
42	32.29551829622564	35.0	32.0	39.0	10.0	40.0
43	32.10101635188853	35.0	31.0	39.0	10.0	40.0
44	31.871573764555503	35.0	31.0	39.0	10.0	40.0
45	31.760267559161083	35.0	31.0	39.0	10.0	40.0
46	31.611579142221707	35.0	31.0	38.0	10.0	40.0
47	31.585013741423435	35.0	31.0	38.0	10.0	40.0
48	31.602736394369394	35.0	31.0	38.0	10.0	40.0
49	31.675863196495598	35.0	31.0	38.0	10.0	40.0
50	31.8162067726166	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	6.0
13	22.0
14	60.0
15	142.0
16	364.0
17	879.0
18	1705.0
19	3071.0
20	4716.0
21	7014.0
22	9930.0
23	14228.0
24	20924.0
25	31946.0
26	45766.0
27	51457.0
28	47937.0
29	45824.0
30	48646.0
31	58158.0
32	76770.0
33	118577.0
34	191129.0
35	137687.0
36	132680.0
37	186277.0
38	203974.0
39	40299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.13768987350324	20.855075949401293	26.826379749708824	25.180854427386645
2	40.45389934846466	18.885539482609058	28.536828906442118	12.123732262484157
3	28.047231582844432	17.834057111355524	41.425594988771756	12.693116317028286
4	25.030468937897414	23.93264135290191	37.21581466238255	13.821075046818123
5	19.47174563368635	27.97163348637613	38.654475488381266	13.902145391556253
6	17.586725001283614	35.8767352431705	36.824852924882926	9.711686830662966
7	73.4920915878708	5.000351304827198	19.130844834062515	2.37671227323949
8	74.97290899313334	3.31739850668425	18.73393793794876	2.97575456223365
9	71.49559923145313	4.772543636113055	20.2835975776181	3.4482595548157136
10	42.13239327764701	22.518774540668936	24.662274455545077	10.68655772613897
11	38.724331102097295	18.13030166275277	30.04743966339593	13.097927571754012
12	34.79762139608538	17.905601690586924	34.64703323073431	12.649743682593387
13	19.629765247305087	32.6727001018784	35.10143251299152	12.59610213782499
14	14.182581225729566	36.16602125664439	36.77127893873514	12.880118578890903
15	12.72149093768663	21.27049390756359	52.835300189974845	13.172714964774935
16	14.987069280014268	16.173195982153715	52.44636521109367	16.39336952673835
17	15.329321252050404	16.321689830265722	37.17865742104424	31.170331496639637
18	19.1848917305546	21.39831481776738	41.76257738839991	17.654216063278106
19	24.400484800661534	22.418990458020424	35.567249202132686	17.613275539185352
20	26.146537350458992	20.34737291547876	33.64000443184551	19.86608530221673
21	20.45222389467341	24.44622198681796	37.18453502103776	17.917019097470877
22	19.012009220400543	20.50039319117198	35.192974443924996	25.294623144502488
23	16.52085264383906	26.587154674110725	34.704458058257146	22.187534623793066
24	17.601723015060166	21.69287040364925	45.174490405324704	15.530916175965887
25	17.46039038073336	20.315012336204123	42.65752643568825	19.567070847374264
26	14.500444535723647	27.553310507527378	36.845052952446835	21.10119200430213
27	15.090231293693538	27.891373845085383	38.87735137779051	18.141043483430572
28	13.73014114347019	24.709160138251963	45.32426786722839	16.236430851049455
29	14.832495156046901	23.283808360514417	42.662525773613766	19.221170709824914
30	17.372631732554336	24.998175917243394	40.79554329291526	16.83364905728701
31	26.524595391421137	22.926761050563574	33.38409237695548	17.164551181059807
32	25.145183475701867	24.511686290194	34.76904409956519	15.57408613453894
33	24.036276276925488	23.82029136681899	32.47333461244322	19.670097743812306
34	17.472483373823465	25.561074344208468	33.553664514699406	23.412777767268658
35	17.293453029193433	23.98999862180414	35.54867058146354	23.167877767538894
36	27.64275812122178	23.83576229093985	30.669857235122915	17.851622352715452
37	18.391618407292007	30.13350934605958	31.714921537418018	19.759950709230402
38	19.133952530610813	31.37307491710557	28.307670065315676	21.18530248696794
39	19.36338160621972	27.526692411005026	30.6259441317231	22.483981851052157
40	21.686722569173273	23.277728084659056	32.054944074973854	22.980605271193816
41	16.46281978873068	23.098967974511485	33.161621839269934	27.276590397487897
42	20.45094028088172	25.04310239995244	28.929074257733433	25.576883061432405
43	20.472018570513633	23.32454620874533	30.338887552729503	25.864547668011532
44	18.57949893122262	26.916300273747527	32.93570581193301	21.568494983096834
45	18.187996724758072	32.411113122856705	27.857054065812907	21.54383608657232
46	22.20030320308932	28.187956189585705	29.787338974027765	19.82440163329721
47	20.73779418401347	25.999664909241748	30.136481925366642	23.126058981378144
48	20.198743950125525	25.046750565465654	33.1373007358485	21.617204748560326
49	20.11895721917908	24.04769368380944	34.15811149334277	21.675237603668705
50	18.139894986880115	28.464338506522108	32.65736429499877	20.738402211599006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	791.0
1	1397.0
2	2003.0
3	27914.5
4	53826.0
5	36978.0
6	20130.0
7	20567.0
8	21004.0
9	22121.5
10	23239.0
11	22968.0
12	22697.0
13	21542.0
14	20387.0
15	18759.5
16	17132.0
17	15940.5
18	14749.0
19	13654.0
20	12559.0
21	11964.0
22	11369.0
23	11039.5
24	10710.0
25	10735.5
26	10761.0
27	11056.0
28	11351.0
29	14063.0
30	16775.0
31	18031.5
32	19288.0
33	22009.0
34	24730.0
35	27808.5
36	30887.0
37	33522.0
38	36157.0
39	45987.5
40	55818.0
41	77938.5
42	100059.0
43	121732.5
44	143406.0
45	143569.5
46	143733.0
47	135721.5
48	127710.0
49	122176.5
50	116643.0
51	106129.0
52	95615.0
53	84134.5
54	72654.0
55	67163.0
56	61672.0
57	55853.5
58	50035.0
59	44584.0
60	39133.0
61	34737.5
62	30342.0
63	26262.0
64	22182.0
65	18523.5
66	14865.0
67	12363.0
68	9861.0
69	8130.0
70	6399.0
71	5344.5
72	4290.0
73	3356.5
74	2423.0
75	1897.0
76	1371.0
77	1044.5
78	718.0
79	562.5
80	407.0
81	272.0
82	137.0
83	114.0
84	91.0
85	64.5
86	38.0
87	29.0
88	20.0
89	18.0
90	16.0
91	11.5
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1480196.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.81982149591483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08625538632465	21.2107925224156
2	7.51179884703122	4.029302083811923
3	2.8473644529987876	2.290974190897219
4	1.6760092045836488	1.7980107076977465
5	1.1988377350203119	1.6076307027905805
6	0.8913316983097078	1.4343214225390184
7	0.6955885464243549	1.3058892454792843
8	0.5387034106305155	1.1558343449880748
9	0.42802605618372097	1.0331624180203027
>10	3.6728159254663826	21.538447547893018
>50	0.9139288426113341	17.45151479982295
>100	0.5317471225620245	20.88003359862376
>500	0.006074217482691512	1.077645870275997
>1k	0.0012654619755607316	0.722774831613682
>5k	0.0	0.0
>10k+	2.530923951121463E-4	2.463665713130972
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36295	2.4520401352253347	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3104	0.2097019583892944	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2152	0.14538615156371185	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2141	0.14464300673694563	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2030	0.13714399984866868	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.048979999945953104	0.0
2	0.0	0.0	0.0	0.20213539288040233	0.0
3	0.0	0.0	0.0	0.2745582341797978	0.0
4	0.0	0.0	0.0	0.4061624271380277	0.0
5	0.0	0.0	0.0	0.694840413026383	0.0
6	0.0	0.0	0.0	0.9750060127172347	0.0
7	0.0	0.0	0.0	1.1711286883628924	0.0
8	0.0	0.0	0.0	1.720785625687409	0.0
9	0.0	0.0	0.0	1.9439993081997249	0.0
10	0.0	0.0	0.0	2.3262459836400042	0.0
11	0.0	0.0	0.0	2.6147212936665145	0.0
12	0.0	0.0	0.0	2.9006293761096504	0.0
13	6.755862061510773E-5	0.0	0.0	3.008047582887672	0.0
14	1.3511724123021546E-4	0.0	0.0	3.0455426173290565	0.0
15	1.3511724123021546E-4	0.0	0.0	3.103035003472513	0.0
16	1.3511724123021546E-4	0.0	0.0	3.2475428929682284	0.0
17	2.026758618453232E-4	0.0	0.0	3.4231953065675085	0.0
18	2.026758618453232E-4	0.0	0.0	3.725587692440731	0.0
19	2.026758618453232E-4	0.0	0.0	3.824628630262479	0.0
20	2.7023448246043093E-4	0.0	0.0	3.954543857705331	0.0
21	2.7023448246043093E-4	0.0	0.0	4.097970809271205	0.0
22	2.7023448246043093E-4	0.0	0.0	4.237141567738327	0.0
23	2.7023448246043093E-4	0.0	0.0	4.403470891692722	0.0
24	2.7023448246043093E-4	0.0	0.0	4.527103167418369	0.0
25	2.7023448246043093E-4	0.0	0.0	4.621482560417674	0.0
26	2.7023448246043093E-4	0.0	0.0	4.722888049960951	0.0
27	2.7023448246043093E-4	0.0	0.0	4.830576491221433	0.0
28	3.3779310307553863E-4	0.0	0.0	4.954141208326464	0.0
29	4.053517236906464E-4	0.0	0.0	5.07655742888104	0.0
30	4.053517236906464E-4	0.0	0.0	5.235793097670849	0.0
31	4.053517236906464E-4	0.0	0.0	5.376720380273964	0.0
32	4.053517236906464E-4	0.0	0.0	5.493123883593794	0.0
33	5.404689649208619E-4	0.0	0.0	5.634388959299985	0.0
34	5.404689649208619E-4	0.0	0.0	5.7753162419030994	0.0
35	6.080275855359696E-4	0.0	0.0	5.968466338241693	0.0
36	6.080275855359696E-4	0.0	0.0	6.1046645174017495	0.0
37	6.080275855359696E-4	0.0	0.0	6.23957908277012	0.0
38	6.080275855359696E-4	0.0	0.0	6.394828792943637	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAC	20	7.857217E-4	44.0	42
CTATCGA	25	4.4434644E-5	44.0	10
CCGGAAC	20	7.857217E-4	44.0	27
TCGATAG	80	0.0	44.0	1
TAGCCGT	40	8.314601E-9	44.0	44
TACGCTA	25	4.4434644E-5	44.0	36
CCGTAAT	25	4.4434644E-5	44.0	14
CGGATTA	20	7.857217E-4	44.0	19
GCGATAC	35	1.4464604E-7	44.0	9
GACGATA	35	1.4464604E-7	44.0	11
TATCGCG	25	4.4434644E-5	44.0	1
TAGTCCG	25	4.4434644E-5	44.0	1
TACGTTC	20	7.857217E-4	44.0	31
GTCGGTA	30	2.5282134E-6	44.0	10
TAGTACG	70	0.0	44.0	1
CGTTTTT	21550	0.0	43.438515	1
CGACGGT	225	0.0	42.044445	28
CGGTCTA	225	0.0	42.044445	31
AGTACGG	95	0.0	41.68421	2
ATTCGGC	70	0.0	40.857143	17
>>END_MODULE