FastQCFastQC Report
Thu 26 May 2016
SRR1546091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546091_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1455366
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362602.4914694997684435No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30070.2066146934860372No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21530.14793529600114336No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20870.14340035427514453No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20480.14072061598250887No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA207.857172E-444.00000420
CTATGCG207.857172E-444.0000041
CTATACG207.857172E-444.0000041
ATTCGGT207.857172E-444.00000442
CCGTATG207.857172E-444.00000435
ATATCGT207.857172E-444.00000417
TCGTACA207.857172E-444.00000437
CGAGTTA254.4434266E-544.031
ATCTAGC254.4434266E-544.035
ACGTTAC454.802132E-1044.01
CGCATCG600.044.021
CGTTCGC254.4434266E-544.043
TACGCTA254.4434266E-544.036
GCGATCG254.4434266E-544.09
GGACCGT254.4434266E-544.08
GACGATA351.4464422E-744.011
ATACCCG254.4434266E-544.036
CGTTTTT218250.043.3044661
CGGTCTA1950.042.8717931
TGCGTAG1000.041.81
Produced by FastQC (version 0.11.5)