##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546091_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1455366
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.89468903354895	31.0	28.0	33.0	23.0	33.0
2	28.7576217941054	31.0	28.0	33.0	16.0	33.0
3	28.197876685314895	31.0	27.0	33.0	16.0	34.0
4	32.022493310960954	35.0	32.0	35.0	25.0	37.0
5	33.63998471862061	35.0	33.0	35.0	28.0	37.0
6	33.4930464226868	35.0	33.0	37.0	28.0	37.0
7	34.224039176399614	35.0	33.0	37.0	30.0	37.0
8	34.73156924100192	35.0	35.0	37.0	32.0	37.0
9	36.624447733422386	39.0	37.0	39.0	32.0	39.0
10	36.389324747177	39.0	35.0	39.0	32.0	39.0
11	36.50543574605975	39.0	35.0	39.0	32.0	39.0
12	35.31231800110763	37.0	34.0	39.0	30.0	39.0
13	35.25046414441453	37.0	34.0	39.0	27.0	39.0
14	36.006252035570434	38.0	34.0	40.0	29.0	41.0
15	36.56593667847126	38.0	35.0	40.0	31.0	41.0
16	36.2170306300958	38.0	34.0	40.0	31.0	41.0
17	35.72163840573437	37.0	34.0	40.0	30.0	41.0
18	35.39130775351355	37.0	34.0	39.0	29.0	40.0
19	34.852869999711416	37.0	33.0	39.0	27.0	40.0
20	35.171442784839	35.0	34.0	39.0	30.0	40.0
21	35.2889403765101	35.0	34.0	39.0	30.0	40.0
22	35.62189442380817	35.0	34.0	39.0	31.0	40.0
23	35.99790018455839	36.0	35.0	40.0	31.0	41.0
24	35.60390307317884	35.0	34.0	40.0	31.0	41.0
25	35.30680598557339	35.0	34.0	39.0	30.0	41.0
26	35.40729204887293	35.0	34.0	40.0	30.0	41.0
27	35.723161046774486	35.0	34.0	40.0	31.0	41.0
28	35.65160035345061	36.0	35.0	40.0	31.0	41.0
29	35.77298150430888	36.0	35.0	40.0	31.0	41.0
30	35.41959617031042	36.0	35.0	40.0	30.0	41.0
31	34.684975463216816	35.0	34.0	39.0	28.0	41.0
32	34.57474477210543	35.0	34.0	40.0	26.0	41.0
33	34.188682434521624	35.0	33.0	40.0	23.0	41.0
34	33.718127261458626	35.0	33.0	40.0	20.0	41.0
35	33.39750275875622	35.0	33.0	40.0	18.0	41.0
36	33.00261240127913	35.0	33.0	40.0	15.0	41.0
37	32.942757354507386	35.0	33.0	40.0	15.0	41.0
38	32.80340477927889	35.0	33.0	40.0	15.0	41.0
39	32.60426861696645	35.0	32.0	40.0	15.0	40.0
40	32.41901349900987	35.0	32.0	39.0	12.0	40.0
41	32.13884686051481	35.0	31.0	39.0	10.0	40.0
42	32.342932980432415	35.0	32.0	39.0	10.0	40.0
43	32.13840092457842	35.0	32.0	39.0	10.0	40.0
44	31.916976210795085	35.0	31.0	39.0	10.0	40.0
45	31.83401013903032	35.0	31.0	39.0	10.0	40.0
46	31.680773770996435	35.0	31.0	39.0	10.0	40.0
47	31.66795225393475	35.0	31.0	38.0	10.0	40.0
48	31.67495736467665	35.0	31.0	38.0	10.0	40.0
49	31.758736977502565	35.0	31.0	38.0	10.0	40.0
50	31.822663852254347	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	9.0
13	22.0
14	58.0
15	142.0
16	366.0
17	849.0
18	1720.0
19	2946.0
20	4765.0
21	7046.0
22	9927.0
23	14219.0
24	20863.0
25	31697.0
26	44801.0
27	50765.0
28	47280.0
29	44596.0
30	47762.0
31	57275.0
32	75842.0
33	116679.0
34	187616.0
35	133743.0
36	128471.0
37	178933.0
38	201698.0
39	45267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.038215816502515	20.836064605054673	26.85551263393538	25.27020694450743
2	40.445427473226665	18.886864197734454	28.525539280153584	12.142169048885297
3	28.105370058115962	17.90965296701998	41.42689880071405	12.558078174150008
4	25.098703693778745	23.926627391322867	37.17264248305924	13.80202643183914
5	19.482315788605753	28.0504010674978	38.60053072560442	13.86675241829203
6	17.58348071893943	35.86444921758513	36.805449625729885	9.746620437745557
7	73.427027977842	5.040038038541508	19.165831825121654	2.367102158494839
8	74.93888135355643	3.342526897014222	18.769986381432574	2.948605367996779
9	71.45199214493124	4.81920011873302	20.298536588047266	3.4302711482884716
10	42.16767466053213	22.668043639881652	24.6808706538424	10.48341104574382
11	38.78907436342473	18.06713912514103	30.089475774478725	13.054310736955516
12	34.77675031572814	18.266745272323252	34.66578166591772	12.290722746030895
13	19.646879204268892	32.70936657857886	35.068566944672334	12.575187272479912
14	14.155958020181863	36.15605971281451	36.87182468190132	12.816157585102303
15	12.714533663696967	21.277122043527193	52.823688336817	13.184655955958846
16	14.982141949172922	16.23921405337214	52.3848296579692	16.393814339485736
17	15.383415580685547	16.35451151119375	37.16247321979488	31.099599688325824
18	19.135942436473023	21.438730875944607	41.770592414554145	17.65473427302823
19	24.39784906339711	22.458611785626434	35.618462984568836	17.525076166407626
20	26.11858460346057	20.50673164001358	33.68300482490315	19.691678931622697
21	20.43657746573714	24.440518742364464	37.32140231391966	17.801501477978736
22	19.068742845442316	20.468459480295678	35.203721950354755	25.259075723907248
23	16.524846670871796	26.513742934766928	34.75201426994997	22.209396124411317
24	17.54672020646353	21.686503601155998	45.28908879278477	15.477687399595702
25	17.463648319391822	20.31887511457599	42.726778006357165	19.490698559675025
26	14.455401596574333	27.59807498594855	36.904874787510494	21.04164862996662
27	15.11269330189107	27.912635034761013	38.879841909182986	18.09482975416493
28	13.706517810640072	24.805718973783915	45.37463428443429	16.11312893114172
29	14.88106771767377	23.263563941991226	42.67703106984772	19.178337270487287
30	17.323271259600677	25.03837522657531	40.759300409656404	16.87905310416761
31	26.451146996700487	22.90942621993368	33.45392155650194	17.185505226863896
32	25.095817821771295	24.45233707534737	34.856043084694846	15.595802018186491
33	24.060820439669474	23.81256673579017	32.52975540173399	19.59685742280636
34	17.457533019185554	25.614999938159887	33.53781797843291	23.389649064221647
35	17.463442185677007	23.98187122689413	35.568853470535934	22.98583311689293
36	27.672351834521354	23.862725939729252	30.64301351000367	17.821908715745728
37	18.446150315453295	30.186702176634604	31.71580207315548	19.651345434756617
38	19.19029302594674	31.31727689117377	28.338438578336994	21.1539915045425
39	19.39999972515505	27.534448379308024	30.638684701992485	22.426867193544442
40	21.72518802830353	23.247691645950226	32.042043032474304	22.98507729327193
41	16.494682437270075	23.06010996546573	33.18574159352355	27.25946600374064
42	20.44915162234105	25.079739392015476	28.98212545847574	25.488983527167736
43	20.370545965757067	23.455611852963447	30.396202742128096	25.77763943915139
44	18.59841441946562	26.840877140183295	33.06549692654631	21.495211513804772
45	18.213356640185356	32.44627124723266	27.959496099263003	21.380876013318986
46	22.18204905157878	28.235440432166204	29.90780326048568	19.674707255769338
47	20.791814567607048	26.02747350151096	30.20202478276942	22.97868714811257
48	20.201035340938294	25.02243421929604	33.16718955918992	21.609340880575743
49	20.178979033452755	24.09400796775519	34.2320076187021	21.495005380089957
50	18.211776281705085	28.428999990380426	32.682637906890776	20.676585821023714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	779.0
1	1435.5
2	2092.0
3	27901.5
4	53711.0
5	36963.5
6	20216.0
7	20528.5
8	20841.0
9	21833.0
10	22825.0
11	22641.5
12	22458.0
13	21182.0
14	19906.0
15	18337.0
16	16768.0
17	15451.0
18	14134.0
19	13350.5
20	12567.0
21	11863.0
22	11159.0
23	10786.5
24	10414.0
25	10392.0
26	10370.0
27	10815.5
28	11261.0
29	14075.5
30	16890.0
31	17986.5
32	19083.0
33	21792.0
34	24501.0
35	27464.5
36	30428.0
37	33214.5
38	36001.0
39	45274.5
40	54548.0
41	76761.5
42	98975.0
43	119752.5
44	140530.0
45	140952.5
46	141375.0
47	133175.0
48	124975.0
49	119648.5
50	114322.0
51	103992.5
52	93663.0
53	82637.0
54	71611.0
55	66241.5
56	60872.0
57	54834.5
58	48797.0
59	43516.0
60	38235.0
61	33828.5
62	29422.0
63	25619.0
64	21816.0
65	18051.0
66	14286.0
67	11970.0
68	9654.0
69	7962.5
70	6271.0
71	5252.5
72	4234.0
73	3367.0
74	2500.0
75	1956.0
76	1412.0
77	1070.0
78	728.0
79	571.0
80	414.0
81	291.0
82	168.0
83	120.5
84	73.0
85	58.5
86	44.0
87	29.5
88	15.0
89	10.5
90	6.0
91	9.0
92	12.0
93	6.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1455366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.510919593684356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91953853030098	20.922295403474838
2	7.398059682510633	3.922587307846348
3	2.9854983991699027	2.3744492402239996
4	1.726687906553024	1.8310433701605757
5	1.177627154023422	1.5609989395827144
6	0.9004912093592286	1.4323710027685257
7	0.700324642743263	1.299637520526966
8	0.5331777069321948	1.1308025054099535
9	0.43181489628259667	1.0303028995232764
>10	3.7425088268384163	21.684941390841168
>50	0.9364520279940465	17.639599720402792
>100	0.5400153275905161	20.87159735750566
>500	0.006242951761453744	1.0723434993014949
>1k	0.0013006149503028634	0.7265041006521703
>5k	0.0	0.0
>10k+	2.6012299006057264E-4	2.500525741779563
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36260	2.4914694997684435	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3007	0.2066146934860372	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2153	0.14793529600114336	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2087	0.14340035427514453	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2048	0.14072061598250887	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.871123827270941E-5	0.0	0.0	0.04658621954889698	0.0
2	6.871123827270941E-5	0.0	0.0	0.19369698069076782	0.0
3	6.871123827270941E-5	0.0	0.0	0.2612401279128412	0.0
4	6.871123827270941E-5	0.0	0.0	0.38739396138153565	0.0
5	1.3742247654541882E-4	0.0	0.0	0.6737824025021885	0.0
6	1.3742247654541882E-4	0.0	0.0	0.9486960668312988	0.0
7	1.3742247654541882E-4	0.0	0.0	1.1421869138072485	0.0
8	1.3742247654541882E-4	0.0	0.0	1.6715382934602017	0.0
9	1.3742247654541882E-4	0.0	0.0	1.8886658064019635	0.0
10	2.0613371481812822E-4	0.0	0.0	2.254827995157232	0.0
11	2.0613371481812822E-4	0.0	0.0	2.5457513780038834	0.0
12	2.0613371481812822E-4	0.0	0.0	2.813244228599541	0.0
13	2.7484495309083764E-4	0.0	0.0	2.9207773164963315	0.0
14	2.7484495309083764E-4	0.0	0.0	2.9583623638315037	0.0
15	2.7484495309083764E-4	0.0	0.0	3.016973050078125	0.0
16	2.7484495309083764E-4	0.0	0.0	3.1608543830211784	0.0
17	2.7484495309083764E-4	0.0	0.0	3.333044746132588	0.0
18	2.7484495309083764E-4	0.0	0.0	3.625342353744694	0.0
19	2.7484495309083764E-4	0.0	0.0	3.71933932770176	0.0
20	3.4355619136354704E-4	0.0	0.0	3.838415903628366	0.0
21	3.4355619136354704E-4	0.0	0.0	3.977556161130602	0.0
22	3.4355619136354704E-4	0.0	0.0	4.113535701672294	0.0
23	4.1226742963625644E-4	0.0	0.0	4.272052528367435	0.0
24	4.1226742963625644E-4	0.0	0.0	4.385219937802587	0.0
25	4.1226742963625644E-4	0.0	0.0	4.475025526225019	0.0
26	4.1226742963625644E-4	0.0	0.0	4.577405271251355	0.0
27	4.1226742963625644E-4	0.0	0.0	4.679304037609783	0.0
28	4.1226742963625644E-4	0.0	0.0	4.797281233724026	0.0
29	4.1226742963625644E-4	0.0	0.0	4.915189718599994	0.0
30	4.1226742963625644E-4	0.0	0.0	5.068278357471591	0.0
31	4.1226742963625644E-4	0.0	0.0	5.20137202600583	0.0
32	4.1226742963625644E-4	0.0	0.0	5.317356596210163	0.0
33	4.8097866790896583E-4	0.0	0.0	5.449350884932038	0.0
34	5.496899061816753E-4	0.0	0.0	5.584162334423094	0.0
35	5.496899061816753E-4	0.0	0.0	5.76817103051741	0.0
36	5.496899061816753E-4	0.0	0.0	5.903051191246738	0.0
37	5.496899061816753E-4	0.0	0.0	6.037038105878521	0.0
38	5.496899061816753E-4	0.0	0.0	6.186759894074755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.857172E-4	44.000004	20
CTATGCG	20	7.857172E-4	44.000004	1
CTATACG	20	7.857172E-4	44.000004	1
ATTCGGT	20	7.857172E-4	44.000004	42
CCGTATG	20	7.857172E-4	44.000004	35
ATATCGT	20	7.857172E-4	44.000004	17
TCGTACA	20	7.857172E-4	44.000004	37
CGAGTTA	25	4.4434266E-5	44.0	31
ATCTAGC	25	4.4434266E-5	44.0	35
ACGTTAC	45	4.802132E-10	44.0	1
CGCATCG	60	0.0	44.0	21
CGTTCGC	25	4.4434266E-5	44.0	43
TACGCTA	25	4.4434266E-5	44.0	36
GCGATCG	25	4.4434266E-5	44.0	9
GGACCGT	25	4.4434266E-5	44.0	8
GACGATA	35	1.4464422E-7	44.0	11
ATACCCG	25	4.4434266E-5	44.0	36
CGTTTTT	21825	0.0	43.304466	1
CGGTCTA	195	0.0	42.87179	31
TGCGTAG	100	0.0	41.8	1
>>END_MODULE