FastQCFastQC Report
Thu 26 May 2016
SRR1546090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546090_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1283128
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192741.502110467544937No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT18920.1474521637747754No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13200.10287360263356424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA207.856824E-444.0000049
TTATCGG408.314601E-944.0000042
ATCGGTA408.314601E-944.00000427
GTAAGTC207.856824E-444.0000049
ATCGAGT207.856824E-444.00000411
CGGCATA351.4462603E-744.06
ACTACGG254.4431312E-544.02
CGTTTTT199350.043.227491
ACGTTAG950.041.6842081
TACGGGA2800.041.6428574
TGTTACG650.040.6153831
CTCGATG650.040.6153831
TAGGCGT603.6379788E-1240.3333325
ACGACGT1150.040.1739126
ATAGGCG2100.039.8095254
CTCGAAT1050.039.80952522
GTTTTTT220100.039.741942
GTAGGCG2550.039.6862754
CTATGCG501.3478711E-939.61
CGTAGGC1500.039.63
Produced by FastQC (version 0.11.5)