##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546090_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1283128
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95367258761402	28.0	25.0	30.0	16.0	33.0
2	26.257720196270363	30.0	25.0	31.0	10.0	33.0
3	26.535184330791626	30.0	25.0	31.0	16.0	33.0
4	27.960042957522553	32.0	19.0	35.0	10.0	37.0
5	31.242981214656684	33.0	30.0	35.0	19.0	37.0
6	29.826765529237925	33.0	28.0	35.0	17.0	37.0
7	29.9397659469671	32.0	28.0	35.0	17.0	35.0
8	32.453015599379015	33.0	32.0	35.0	28.0	35.0
9	27.19342653266081	31.0	23.0	34.0	10.0	35.0
10	31.699692470275764	34.0	30.0	37.0	17.0	39.0
11	33.83643486854	35.0	32.0	37.0	27.0	39.0
12	33.25764148237744	35.0	31.0	39.0	25.0	39.0
13	33.09015234645335	35.0	31.0	38.0	22.0	39.0
14	32.84933459483388	35.0	31.0	39.0	21.0	40.0
15	31.905908062173065	34.0	27.0	38.0	18.0	40.0
16	30.55567020593425	34.0	27.0	38.0	17.0	40.0
17	30.513636987112744	32.0	26.0	38.0	17.0	40.0
18	31.59233139640005	34.0	29.0	38.0	18.0	39.0
19	31.330502490788138	34.0	28.0	37.0	18.0	39.0
20	31.622874724891048	34.0	29.0	36.0	19.0	39.0
21	32.543483580749545	34.0	31.0	37.0	23.0	39.0
22	32.86953367084188	34.0	31.0	37.0	25.0	39.0
23	34.204796403788244	35.0	33.0	38.0	28.0	40.0
24	33.81263521643982	35.0	33.0	38.0	26.0	40.0
25	32.720655304848776	35.0	31.0	37.0	23.0	39.0
26	26.290202536301912	33.0	20.0	36.0	0.0	39.0
27	29.53426392378625	34.0	23.0	37.0	12.0	39.0
28	31.035956662156853	34.0	27.0	36.0	18.0	39.0
29	32.34245297429407	34.0	30.0	37.0	23.0	39.0
30	32.112814154160766	34.0	30.0	37.0	22.0	39.0
31	30.999591622971362	34.0	29.0	36.0	18.0	38.0
32	31.18706239751607	34.0	30.0	37.0	18.0	39.0
33	30.79867324226422	34.0	29.0	37.0	17.0	38.0
34	30.707957429032803	34.0	29.0	37.0	15.0	39.0
35	29.83122884077037	34.0	27.0	37.0	10.0	39.0
36	29.55143836000773	33.0	25.0	37.0	10.0	39.0
37	29.683397915094986	34.0	26.0	37.0	10.0	39.0
38	29.438863464907634	33.0	26.0	36.0	10.0	38.0
39	29.457247445305534	33.0	26.0	37.0	10.0	39.0
40	29.317351035906004	33.0	25.0	36.0	10.0	38.0
41	28.735237638022085	33.0	24.0	36.0	9.0	38.0
42	28.909897531657013	33.0	24.0	36.0	8.0	38.0
43	28.524639007176212	33.0	24.0	35.0	8.0	38.0
44	28.179847996458655	32.0	23.0	35.0	8.0	38.0
45	27.527908361441728	31.0	23.0	35.0	8.0	38.0
46	27.16284657493251	31.0	23.0	35.0	8.0	38.0
47	27.26294492833139	31.0	23.0	34.0	8.0	37.0
48	27.346216433590413	31.0	23.0	34.0	8.0	37.0
49	27.196283613170316	31.0	23.0	34.0	8.0	37.0
50	28.948393301369777	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	20.0
12	66.0
13	171.0
14	414.0
15	926.0
16	1920.0
17	3565.0
18	6259.0
19	9975.0
20	14417.0
21	20411.0
22	27339.0
23	36105.0
24	46195.0
25	57145.0
26	67416.0
27	72918.0
28	79846.0
29	91815.0
30	108898.0
31	125868.0
32	135799.0
33	131806.0
34	111819.0
35	83154.0
36	40353.0
37	8275.0
38	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.1887574739231	21.492243953837807	26.680190908467434	26.638807663771658
2	40.49182934204538	16.22223192074368	30.239539625041306	13.046399112169635
3	29.307676241185604	13.720377078514382	43.32373699272403	13.648209687575985
4	26.351930594609424	23.792014514530116	36.2257701491979	13.630284741662562
5	18.611003734623512	28.157596124470825	39.590438366242495	13.640961774663166
6	17.447674744842292	34.3017999763079	38.287060994694215	9.963464284155595
7	75.5543484360095	3.661053300995692	19.212658440934966	1.5719398220598413
8	77.73020306625683	1.9725233959511443	18.462070814447195	1.835202723344826
9	65.9570985903199	3.0382003977779304	18.582479690256935	12.42222132164523
10	43.53961568915962	22.352173750397466	24.659192224002595	9.449018336440323
11	40.132706947397295	17.409253012949605	30.189817383768414	12.268222655884681
12	35.706881932277994	17.932973171811387	35.081301319899495	11.278843576011123
13	18.89546483281481	33.76358399162048	35.49443235593019	11.846518819634518
14	13.398117724809996	36.658384822091016	37.63030656333585	12.313190889763142
15	11.192725901079239	19.54637417311445	53.49700107861414	15.763898847192173
16	13.143505558291924	14.158057496991727	53.123304923592976	19.57513202112338
17	14.390302448391742	15.170816941100188	38.007665642087154	32.431214968420925
18	18.636488331639555	20.899317916840722	43.07395676814784	17.390236983371885
19	25.084325180340546	21.529886340256	36.4496761040208	16.93611237538266
20	26.82974730502335	19.82296388201333	34.27561396836481	19.071674844598512
21	20.23765360899302	23.993241516045163	37.99956044915238	17.76954442580943
22	18.64584047733352	20.027230330878915	35.4691815625565	25.857747629231064
23	15.825778877867211	26.549104999657086	35.4123672774657	22.212748845010008
24	16.68126640522224	21.056667768141605	47.51607010368412	14.745995722952035
25	16.8079879793754	19.021718799683274	44.754225611162724	19.416067609778604
26	11.415384903142945	39.76438827615016	30.75351796547188	18.06670885523502
27	14.056820519854604	28.859007051517853	39.672503444707	17.411668983920546
28	12.917417436140433	24.503245194555802	47.166767462014704	15.412569907289061
29	14.05565150164286	23.008304705376236	44.09645803068751	18.839585762293396
30	16.803078102886072	24.91037527043288	41.849838831355875	16.436707795325173
31	27.49258063108279	22.370800107237937	33.814553185652564	16.322066076026708
32	25.641868932795482	23.541844617216675	35.62925912301812	15.187027326969718
33	24.568008803486478	23.25543515533914	32.949557643508676	19.226998397665703
34	17.138274591467102	24.672441097069036	33.75493325685356	24.434351054610296
35	17.170695363206164	23.34256597938787	36.06358835595513	23.42315030145083
36	28.440966138997826	22.8985728625671	30.518311501268773	18.142149497166297
37	17.84412778771876	30.621652711187036	31.348470300702658	20.18574920039154
38	18.58590881034472	32.152365157646	28.14224301862324	21.119483013386038
39	19.016497185004148	27.655853507989846	30.365403919172522	22.962245387833484
40	21.849807657536893	22.413586173787806	32.160937957865464	23.575668210809834
41	15.804268942771103	22.33541782269579	33.34094494080092	28.519368293732196
42	19.855150850110043	24.27302654138948	28.125019483636866	27.746803124863618
43	20.00213540660012	22.4972099432013	30.31420092149809	27.18645372870049
44	17.848258318733595	26.446543135213325	33.27220666995031	22.432991876102772
45	17.507996084568337	33.63616100654026	27.30429076444439	21.551552144447008
46	21.552643228111304	27.98629598917645	29.470559445355416	20.990501337356836
47	20.353464346503234	25.367539325772643	29.59798243043562	24.6810138972885
48	19.606851381935396	24.400917133754387	33.84728569558143	22.144945788728794
49	19.743470643614668	23.88561390601717	33.86100217593256	22.509913274435597
50	17.658487695693648	29.165367757542505	32.545934622266834	20.630209924497013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	521.0
1	922.5
2	1324.0
3	16906.5
4	32489.0
5	25180.5
6	17872.0
7	17671.5
8	17471.0
9	18862.5
10	20254.0
11	20950.5
12	21647.0
13	21307.5
14	20968.0
15	19870.0
16	18772.0
17	17888.5
18	17005.0
19	16013.0
20	15021.0
21	14346.5
22	13672.0
23	12992.0
24	12312.0
25	11864.0
26	11416.0
27	11340.0
28	11264.0
29	13528.5
30	15793.0
31	16810.0
32	17827.0
33	19924.5
34	22022.0
35	23909.0
36	25796.0
37	28310.5
38	30825.0
39	38693.5
40	46562.0
41	64724.5
42	82887.0
43	100364.0
44	117841.0
45	119593.0
46	121345.0
47	115423.0
48	109501.0
49	103697.5
50	97894.0
51	89758.5
52	81623.0
53	72914.5
54	64206.0
55	58930.0
56	53654.0
57	48727.5
58	43801.0
59	39356.5
60	34912.0
61	30741.0
62	26570.0
63	23241.0
64	19912.0
65	16834.0
66	13756.0
67	11434.5
68	9113.0
69	7509.0
70	5905.0
71	5010.5
72	4116.0
73	3236.5
74	2357.0
75	1843.0
76	1329.0
77	1046.5
78	764.0
79	577.0
80	390.0
81	303.0
82	216.0
83	158.0
84	100.0
85	72.0
86	44.0
87	35.5
88	27.0
89	18.5
90	10.0
91	11.0
92	12.0
93	8.5
94	5.0
95	4.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1283128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.728371141608406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.21422412528172	33.89179804435457
2	6.969352292818716	5.677007335970803
3	2.64540283012635	3.232288448533417
4	1.3935316573434147	2.2702509815145304
5	0.9132479222035617	1.8597550159904688
6	0.6175781281594486	1.509177072757069
7	0.4547359437081795	1.2964458000743397
8	0.35767935504905346	1.165415801770318
9	0.2865170218568942	1.0502434444118323
>10	2.4924825581002668	21.97284065405896
>50	0.4798237264494405	13.647854623224385
>100	0.17354040556779277	10.118179004238065
>500	0.001046685186155978	0.2884331099524259
>1k	6.280111116935867E-4	0.37701779846574857
>5k	0.0	0.0
>10k+	2.0933703723119554E-4	1.6432928646831384
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19274	1.502110467544937	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1892	0.1474521637747754	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1320	0.10287360263356424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037564451870740874	0.0
2	0.0	0.0	0.0	0.15454420759269535	0.0
3	0.0	0.0	0.0	0.20450025250793374	0.0
4	0.0	0.0	0.0	0.2940470475276044	0.0
5	0.0	0.0	0.0	0.4902083034584235	0.0
6	0.0	0.0	0.0	0.6529356385333341	0.0
7	0.0	0.0	0.0	0.7696815906129396	0.0
8	0.0	0.0	0.0	1.0864855259958477	0.0
9	0.0	0.0	0.0	1.2543565412024364	0.0
10	0.0	0.0	0.0	1.5017207948076887	0.0
11	0.0	0.0	0.0	1.7041947490819311	0.0
12	0.0	0.0	0.0	1.8933418957422798	0.0
13	0.0	0.0	0.0	1.9661327630602714	0.0
14	0.0	0.0	0.0	1.991773229171213	0.0
15	0.0	0.0	0.0	2.029415615589403	0.0
16	0.0	0.0	0.0	2.1142084032146444	0.0
17	0.0	0.0	0.0	2.2463074611418348	0.0
18	0.0	0.0	0.0	2.4335062441159416	0.0
19	0.0	0.0	0.0	2.4864238018342677	0.0
20	0.0	0.0	0.0	2.5617865092180985	0.0
21	0.0	0.0	0.0	2.649229071456628	0.0
22	0.0	0.0	0.0	2.733164579059922	0.0
23	0.0	0.0	0.0	2.828868203328117	0.0
24	0.0	0.0	0.0	2.8976064741787257	0.0
25	0.0	0.0	0.0	2.951848919203696	0.0
26	0.0	0.0	0.0	3.00983222250625	0.0
27	0.0	0.0	0.0	3.083792108035987	0.0
28	0.0	0.0	0.0	3.163129477339751	0.0
29	0.0	0.0	0.0	3.24838987224969	0.0
30	0.0	0.0	0.0	3.3568747622996304	0.0
31	0.0	0.0	0.0	3.4537474047795698	0.0
32	0.0	0.0	0.0	3.539631276069106	0.0
33	0.0	0.0	0.0	3.640478580468979	0.0
34	0.0	0.0	0.0	3.7424949030805967	0.0
35	0.0	0.0	0.0	3.8851930594609425	0.0
36	0.0	0.0	0.0	3.9911840439924933	0.0
37	0.0	0.0	0.0	4.093200366604111	0.0
38	0.0	0.0	0.0	4.203243947603045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	20	7.856824E-4	44.000004	9
TTATCGG	40	8.314601E-9	44.000004	2
ATCGGTA	40	8.314601E-9	44.000004	27
GTAAGTC	20	7.856824E-4	44.000004	9
ATCGAGT	20	7.856824E-4	44.000004	11
CGGCATA	35	1.4462603E-7	44.0	6
ACTACGG	25	4.4431312E-5	44.0	2
CGTTTTT	19935	0.0	43.22749	1
ACGTTAG	95	0.0	41.684208	1
TACGGGA	280	0.0	41.642857	4
TGTTACG	65	0.0	40.615383	1
CTCGATG	65	0.0	40.615383	1
TAGGCGT	60	3.6379788E-12	40.333332	5
ACGACGT	115	0.0	40.17391	26
ATAGGCG	210	0.0	39.809525	4
CTCGAAT	105	0.0	39.809525	22
GTTTTTT	22010	0.0	39.74194	2
GTAGGCG	255	0.0	39.686275	4
CTATGCG	50	1.3478711E-9	39.6	1
CGTAGGC	150	0.0	39.6	3
>>END_MODULE