FastQCFastQC Report
Thu 26 May 2016
SRR1546089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546089_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences452215
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82431.8228055239211436No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8150.1802240084915361No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5800.12825757659520362No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5800.12825757659520362No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5790.12803644284245325No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.851409E-444.0000041
TTGATCG207.851409E-444.0000041
TAATACG207.851409E-444.0000041
AGTCCGG207.851409E-444.00000412
CAAACGA207.851409E-444.00000424
GTACACT408.292773E-944.00000418
CTATATG207.851409E-444.0000041
TAAACGG207.851409E-444.0000042
CCTACGT207.851409E-444.00000431
CGCTCCA207.851409E-444.00000415
CTACGTG207.851409E-444.00000432
ATTGGAT207.851409E-444.00000417
GCTACGT207.851409E-444.00000429
CACGTGA207.851409E-444.00000443
GATTCGC207.851409E-444.0000049
TGCACAT207.851409E-444.00000432
AGTCGAG207.851409E-444.0000041
TCTATAC207.851409E-444.00000425
GTTTGCG351.4434772E-744.01
TACCAAC302.524297E-644.044
Produced by FastQC (version 0.11.5)