##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546089_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	452215
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.232192651725395	31.0	28.0	33.0	25.0	33.0
2	28.893108366595534	31.0	28.0	33.0	16.0	33.0
3	28.32945612153511	31.0	28.0	33.0	16.0	34.0
4	32.154079364903865	35.0	32.0	35.0	25.0	37.0
5	33.703950554492884	35.0	33.0	35.0	28.0	37.0
6	33.55689439757637	35.0	33.0	37.0	28.0	37.0
7	34.311186050882874	35.0	35.0	37.0	31.0	37.0
8	34.81302920071205	35.0	35.0	37.0	32.0	37.0
9	36.65750583240273	39.0	37.0	39.0	32.0	39.0
10	36.453206992249264	39.0	35.0	39.0	32.0	39.0
11	36.49930232301007	39.0	35.0	39.0	32.0	39.0
12	35.17574162732328	37.0	34.0	39.0	30.0	39.0
13	34.97896796877591	37.0	33.0	39.0	27.0	39.0
14	35.749409020045775	38.0	33.0	40.0	27.0	41.0
15	36.424253950001656	38.0	35.0	40.0	31.0	41.0
16	36.05406720254746	37.0	34.0	40.0	31.0	41.0
17	35.49926915294716	36.0	34.0	40.0	29.0	41.0
18	35.220361995953255	36.0	33.0	39.0	29.0	40.0
19	34.6428866800084	36.0	33.0	39.0	27.0	40.0
20	34.948228165806086	35.0	34.0	39.0	29.0	40.0
21	35.15582632155059	35.0	34.0	39.0	30.0	40.0
22	35.444633636655134	35.0	34.0	39.0	31.0	40.0
23	35.84448326570326	35.0	35.0	40.0	31.0	41.0
24	35.5958913348739	35.0	34.0	40.0	31.0	41.0
25	35.2101787866391	35.0	34.0	39.0	30.0	41.0
26	35.33183773205223	35.0	34.0	39.0	31.0	41.0
27	35.58810300410203	35.0	34.0	40.0	31.0	41.0
28	35.437294207401344	35.0	35.0	40.0	31.0	41.0
29	35.4902756432228	36.0	35.0	40.0	31.0	41.0
30	35.10190064460489	36.0	34.0	40.0	30.0	41.0
31	34.40726203244032	35.0	34.0	39.0	27.0	41.0
32	34.19573432990945	35.0	34.0	39.0	25.0	41.0
33	33.78661698528355	35.0	33.0	40.0	22.0	41.0
34	33.37854339197063	35.0	33.0	40.0	18.0	41.0
35	33.05303450792211	35.0	33.0	40.0	16.0	41.0
36	32.60871266985836	35.0	33.0	39.0	15.0	41.0
37	32.46359585595347	35.0	32.0	39.0	14.0	40.0
38	32.34957929303539	35.0	32.0	39.0	12.0	40.0
39	32.131382196521564	35.0	31.0	39.0	12.0	40.0
40	31.94917240693033	35.0	31.0	39.0	10.0	40.0
41	31.701186382583504	35.0	31.0	38.0	10.0	40.0
42	31.79893634664927	35.0	31.0	39.0	10.0	40.0
43	31.56248687018343	35.0	31.0	38.0	10.0	40.0
44	31.37289342458786	35.0	30.0	38.0	10.0	40.0
45	31.269741162942406	35.0	30.0	38.0	10.0	40.0
46	31.115785632940085	35.0	30.0	38.0	10.0	40.0
47	31.167449111595147	35.0	30.0	38.0	10.0	40.0
48	31.24691131430846	35.0	31.0	38.0	10.0	40.0
49	31.115818803002995	35.0	30.0	38.0	10.0	40.0
50	31.193848058998487	35.0	30.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	8.0
14	21.0
15	34.0
16	105.0
17	250.0
18	537.0
19	838.0
20	1465.0
21	2244.0
22	3289.0
23	4857.0
24	6989.0
25	10659.0
26	15005.0
27	16853.0
28	15274.0
29	14528.0
30	15045.0
31	18269.0
32	24753.0
33	38712.0
34	64300.0
35	42329.0
36	37559.0
37	50593.0
38	55591.0
39	12100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.897416052099114	22.018951162610705	26.38567937817189	25.697953407118295
2	40.06390765454485	20.0650133233086	28.06320002653605	11.807878995610494
3	27.177780480523644	18.09625952257223	42.25379520803158	12.47216478887255
4	24.54739449155822	23.789569120882767	38.0162091040766	13.646827283482416
5	19.19418860497772	27.834326592439435	38.748382959433016	14.223101843149829
6	17.19735081764205	35.32036752429707	37.1347699656137	10.347511692447178
7	74.39735524031711	4.683391749499685	18.68845571243767	2.2307972977455415
8	76.09123978638479	3.181672434572051	18.232699048019192	2.49438873102396
9	73.57142067379453	3.9684663268578	19.268931813407338	3.191181185940316
10	46.44405868889798	20.64438375551452	23.718364052497154	9.193193503090344
11	41.43007197903652	17.753502205809184	28.466105724047193	12.350320091107106
12	36.75021836958084	17.78092279115023	34.38010680760258	11.088752031666353
13	19.32598432161693	36.02733213183995	32.50046990922459	12.146213637318532
14	12.227811992083412	39.4022754663158	35.95546366219608	12.414448879404707
15	11.021969638335747	20.509049898831307	55.62840684187831	12.840573620954634
16	12.672733102617117	14.562984421127117	54.04929071348805	18.71499176276771
17	12.615017193149278	14.21757349933107	37.32472385922625	35.8426854482934
18	18.82688544165939	19.966387669581948	42.0773304733368	19.12939641542187
19	25.980562343133247	21.491547162301117	33.34232610594518	19.185564388620456
20	26.7405990513362	18.503809028891123	33.13003770330484	21.62555421646783
21	20.4110876463629	24.29463861216457	36.015390909191424	19.278882832281106
22	18.610616631469547	20.5358070829141	32.82973806706987	28.023838218546487
23	16.090576385126543	26.55639463529516	33.003328062978895	24.349700916599403
24	16.504981037780702	20.482292714748514	46.193514147031834	16.819212100438953
25	16.01295843791117	20.898466437424677	42.60318653737713	20.48538858728702
26	13.626482978229381	28.27769976670389	35.298254149022036	22.79756310604469
27	14.043320102163795	28.675740521654525	37.70993885651737	19.57100051966432
28	13.185321141492432	23.329168647656534	47.541324370045224	15.94418584080581
29	13.175812390124166	20.772420198356976	45.68468538195327	20.367082029565584
30	17.046316464513563	25.341928065190228	40.81045520383004	16.80130026646617
31	27.371935915438456	23.33314905520604	32.3761927401789	16.918722289176607
32	26.577402341806444	23.776522229470494	33.165419103744895	16.480656324978163
33	24.481275499485864	23.390201563415634	32.310516015612045	19.81800692148646
34	18.13318885928154	24.058246630474443	32.924604446999766	24.883960063244253
35	19.083179461097043	22.889554747188836	35.413464834204966	22.61380095750915
36	30.005196643189635	22.341143040367967	30.19316033302743	17.46049998341497
37	19.053326404475747	30.075959444069746	31.473082493946464	19.397631657508043
38	20.040467476753314	30.6133144632531	28.099687095739856	21.24653096425373
39	20.18309874727729	27.858651305241978	29.673053746558608	22.285196200922126
40	23.26216512057318	23.1810090333138	31.18560861537101	22.371217230742012
41	17.205753900246563	21.729265946507745	32.283979965282	28.78100018796369
42	22.232124100262045	24.004511128556107	27.11895890229205	26.6444058688898
43	21.19036299105514	24.28955253585131	28.02074234600798	26.49934212708557
44	18.749709761949514	27.083798635604744	32.045818913569875	22.120672688875867
45	17.92156385789945	34.06344327366407	26.263834680406443	21.75115818803003
46	22.033103722786727	29.485311190473556	28.539964397465805	19.941620689273908
47	21.208495958780667	25.09757526840109	29.568678615260442	24.125250157557797
48	20.966796767024533	23.630795086408014	32.38326902026691	23.01913912630054
49	19.271143150934844	24.101146578508008	34.47939586258749	22.14831440796966
50	18.535652289287174	28.921198987207415	31.983459195294273	20.559689528211138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	397.0
2	533.0
3	6545.0
4	12557.0
5	9172.0
6	5787.0
7	6302.5
8	6818.0
9	7341.0
10	7864.0
11	7746.5
12	7629.0
13	7177.5
14	6726.0
15	6203.0
16	5680.0
17	5159.0
18	4638.0
19	4247.0
20	3856.0
21	3471.0
22	3086.0
23	2930.0
24	2774.0
25	2588.5
26	2403.0
27	2571.5
28	2740.0
29	3232.5
30	3725.0
31	4712.5
32	5700.0
33	6361.0
34	7022.0
35	8322.5
36	9623.0
37	9450.0
38	9277.0
39	12738.0
40	16199.0
41	22648.0
42	29097.0
43	35874.0
44	42651.0
45	41775.0
46	40899.0
47	40726.0
48	40553.0
49	40366.5
50	40180.0
51	36900.0
52	33620.0
53	28717.5
54	23815.0
55	20469.0
56	17123.0
57	15332.0
58	13541.0
59	13128.0
60	12715.0
61	11402.0
62	10089.0
63	8749.5
64	7410.0
65	6360.5
66	5311.0
67	4583.0
68	3855.0
69	3154.0
70	2453.0
71	2142.0
72	1831.0
73	1476.0
74	1121.0
75	818.0
76	515.0
77	395.0
78	275.0
79	192.0
80	109.0
81	88.0
82	67.0
83	50.0
84	33.0
85	28.0
86	23.0
87	18.0
88	13.0
89	9.5
90	6.0
91	6.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	452215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.347208118412034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4813491978031	25.386525359151058
2	7.401069688064202	4.788078829973671
3	3.1412217799999214	3.048292639912385
4	1.7927608138729463	2.319632286115277
5	1.0976170476375955	1.7752423537125135
6	0.8063605886689079	1.5650108268095047
7	0.5992663358677096	1.3569215019269707
8	0.4680898266835004	1.2113119233474083
9	0.394062428956653	1.147213746099703
>10	5.082210636192146	38.265234856231224
>50	0.6723789945139689	13.857338760931814
>100	0.06019262616198297	2.8902828713723823
>500	0.002736028461908317	0.5650909020504526
>1k	0.0	0.0
>5k	6.840071154770792E-4	1.8238231423656543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8243	1.8228055239211436	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	815	0.1802240084915361	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	580	0.12825757659520362	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	580	0.12825757659520362	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	579	0.12803644284245325	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2113375275035105E-4	0.0	0.0	0.05727364196234092	0.0
2	2.2113375275035105E-4	0.0	0.0	0.19371316740930752	0.0
3	2.2113375275035105E-4	0.0	0.0	0.26779297458067514	0.0
4	2.2113375275035105E-4	0.0	0.0	0.38388819477460945	0.0
5	2.2113375275035105E-4	0.0	0.0	0.620501310217485	0.0
6	2.2113375275035105E-4	0.0	0.0	0.8761319283968909	0.0
7	2.2113375275035105E-4	0.0	0.0	1.0214168039538716	0.0
8	2.2113375275035105E-4	0.0	0.0	1.422332297690258	0.0
9	2.2113375275035105E-4	0.0	0.0	1.6217949426710745	0.0
10	2.2113375275035105E-4	0.0	0.0	1.96543679444512	0.0
11	2.2113375275035105E-4	0.0	0.0	2.2150968013002665	0.0
12	2.2113375275035105E-4	0.0	0.0	2.5231361188815056	0.0
13	4.422675055007021E-4	0.0	0.0	2.8386939840562566	0.0
14	4.422675055007021E-4	0.0	0.0	3.027984476410557	0.0
15	4.422675055007021E-4	0.0	0.0	3.126831263889964	0.0
16	4.422675055007021E-4	0.0	0.0	3.239609477792643	0.0
17	4.422675055007021E-4	0.0	0.0	3.3627809780745883	0.0
18	4.422675055007021E-4	0.0	0.0	3.632564156430017	0.0
19	4.422675055007021E-4	0.0	0.0	3.6997888172661235	0.0
20	4.422675055007021E-4	0.0	0.0	3.8138938336853045	0.0
21	4.422675055007021E-4	0.0	0.0	3.9036741373019472	0.0
22	4.422675055007021E-4	0.0	0.0	3.977975078226065	0.0
23	4.422675055007021E-4	0.0	0.0	4.090753292128744	0.0
24	4.422675055007021E-4	0.0	0.0	4.163064029278109	0.0
25	4.422675055007021E-4	0.0	0.0	4.224096945037206	0.0
26	4.422675055007021E-4	0.0	0.0	4.292206140884314	0.0
27	4.422675055007021E-4	0.0	0.0	4.362084406753425	0.0
28	6.634012582510531E-4	0.0	0.0	4.421127118737768	0.0
29	6.634012582510531E-4	0.0	0.0	4.491668785865131	0.0
30	6.634012582510531E-4	0.0	0.0	4.583218159503776	0.0
31	6.634012582510531E-4	0.0	0.0	4.661941775482901	0.0
32	6.634012582510531E-4	0.0	0.0	4.727839633802505	0.0
33	6.634012582510531E-4	0.0	0.0	4.844377121501941	0.0
34	6.634012582510531E-4	0.0	0.0	4.965558418009133	0.0
35	6.634012582510531E-4	0.0	0.0	5.100892274692348	0.0
36	8.845350110014042E-4	0.0	0.0	5.176962285638468	0.0
37	8.845350110014042E-4	0.0	0.0	5.2601085766726	0.0
38	8.845350110014042E-4	0.0	0.0	5.336620855124222	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.851409E-4	44.000004	1
TTGATCG	20	7.851409E-4	44.000004	1
TAATACG	20	7.851409E-4	44.000004	1
AGTCCGG	20	7.851409E-4	44.000004	12
CAAACGA	20	7.851409E-4	44.000004	24
GTACACT	40	8.292773E-9	44.000004	18
CTATATG	20	7.851409E-4	44.000004	1
TAAACGG	20	7.851409E-4	44.000004	2
CCTACGT	20	7.851409E-4	44.000004	31
CGCTCCA	20	7.851409E-4	44.000004	15
CTACGTG	20	7.851409E-4	44.000004	32
ATTGGAT	20	7.851409E-4	44.000004	17
GCTACGT	20	7.851409E-4	44.000004	29
CACGTGA	20	7.851409E-4	44.000004	43
GATTCGC	20	7.851409E-4	44.000004	9
TGCACAT	20	7.851409E-4	44.000004	32
AGTCGAG	20	7.851409E-4	44.000004	1
TCTATAC	20	7.851409E-4	44.000004	25
GTTTGCG	35	1.4434772E-7	44.0	1
TACCAAC	30	2.524297E-6	44.0	44
>>END_MODULE