FastQCFastQC Report
Thu 26 May 2016
SRR1546088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546088_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences454049
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86201.898473512770648No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7560.1665018533242007No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6100.13434673350233123No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5620.12377518725952485No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5590.12311446561934945No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATT207.851443E-444.00000434
TCTTGAG207.851443E-444.0000041
CGACGGT650.044.00000428
ACGTTAG207.851443E-444.0000041
GGCGTAG207.851443E-444.0000041
TTCACGA207.851443E-444.00000411
CACGATA207.851443E-444.00000413
GCTTGTA207.851443E-444.00000442
AGGCGAG207.851443E-444.0000041
AACGGGA800.044.0000044
GTTACAC207.851443E-444.00000433
GTTAAGC207.851443E-444.00000437
CTACGTC207.851443E-444.00000430
ACTACGG207.851443E-444.0000042
GCTACGA207.851443E-444.00000410
CACGTGC207.851443E-444.00000441
AACTACG207.851443E-444.0000041
TAAGCTT207.851443E-444.00000439
TGTAACG207.851443E-444.0000041
TACGTCC207.851443E-444.00000431
Produced by FastQC (version 0.11.5)