##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546088_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	454049
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.22330409273008	31.0	28.0	33.0	25.0	33.0
2	28.852731753621306	31.0	28.0	33.0	16.0	33.0
3	28.340738554649388	31.0	28.0	33.0	16.0	34.0
4	32.12646872914598	35.0	32.0	35.0	25.0	37.0
5	33.733766619902255	35.0	33.0	35.0	30.0	37.0
6	33.63011260899154	35.0	33.0	37.0	28.0	37.0
7	34.377952599829534	35.0	35.0	37.0	31.0	37.0
8	34.84604084581179	35.0	35.0	37.0	32.0	37.0
9	36.76643049538706	39.0	37.0	39.0	32.0	39.0
10	36.44340588791078	39.0	35.0	39.0	32.0	39.0
11	36.54089756832413	39.0	35.0	39.0	32.0	39.0
12	35.10682987959449	37.0	34.0	39.0	30.0	39.0
13	34.991364368162905	37.0	33.0	39.0	27.0	39.0
14	35.76077692055263	38.0	33.0	40.0	27.0	41.0
15	36.472909311550076	38.0	35.0	40.0	31.0	41.0
16	36.080577206424856	37.0	34.0	40.0	31.0	41.0
17	35.55376842587474	36.0	34.0	40.0	30.0	41.0
18	35.29251908934939	36.0	34.0	39.0	29.0	40.0
19	34.69923950939216	36.0	33.0	39.0	27.0	40.0
20	34.90849005283571	35.0	34.0	39.0	29.0	40.0
21	35.056885930813635	35.0	34.0	39.0	30.0	40.0
22	35.411426960526285	35.0	34.0	39.0	31.0	40.0
23	35.86019570574982	35.0	35.0	40.0	31.0	41.0
24	35.483183533054806	35.0	34.0	39.0	31.0	41.0
25	35.122559459441604	35.0	34.0	39.0	30.0	41.0
26	35.28820898184998	35.0	34.0	39.0	30.0	41.0
27	35.60280938841402	35.0	34.0	40.0	31.0	41.0
28	35.45562923825402	35.0	35.0	40.0	31.0	41.0
29	35.51257463401527	36.0	35.0	40.0	31.0	41.0
30	35.134086849657194	36.0	34.0	40.0	30.0	41.0
31	34.43556091963643	35.0	34.0	39.0	27.0	41.0
32	34.27391537036752	35.0	34.0	39.0	25.0	41.0
33	33.861391611918535	35.0	33.0	40.0	23.0	41.0
34	33.360820087699786	35.0	33.0	40.0	18.0	41.0
35	33.053802563159486	35.0	33.0	40.0	16.0	41.0
36	32.582802737149514	35.0	33.0	39.0	15.0	41.0
37	32.4331823217318	35.0	32.0	39.0	13.0	40.0
38	32.34782809784847	35.0	32.0	39.0	12.0	40.0
39	32.09247239835348	35.0	31.0	39.0	10.0	40.0
40	31.916649965091874	35.0	31.0	39.0	10.0	40.0
41	31.66454281366108	35.0	31.0	38.0	10.0	40.0
42	31.768569031095762	35.0	31.0	39.0	10.0	40.0
43	31.53634739862878	35.0	31.0	38.0	10.0	40.0
44	31.338672698321105	35.0	30.0	38.0	10.0	40.0
45	31.2252664360014	35.0	30.0	38.0	10.0	40.0
46	31.056454259342054	35.0	30.0	38.0	10.0	40.0
47	31.123295062867665	35.0	30.0	38.0	10.0	40.0
48	31.19610879002046	35.0	30.0	38.0	10.0	40.0
49	31.134366555151537	35.0	30.0	38.0	10.0	40.0
50	31.221520144301607	35.0	30.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	6.0
14	15.0
15	41.0
16	103.0
17	238.0
18	495.0
19	871.0
20	1450.0
21	2198.0
22	3282.0
23	4699.0
24	6726.0
25	10594.0
26	15211.0
27	17355.0
28	15659.0
29	14546.0
30	14993.0
31	17899.0
32	24530.0
33	39247.0
34	65324.0
35	42300.0
36	38168.0
37	51678.0
38	55830.0
39	10587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.993450046140392	21.86283859231052	26.281304440710144	25.862406920838943
2	40.271204209237325	20.010615594352153	27.90601895390145	11.812161242509069
3	27.135177040363484	18.006646859700165	42.34124510790686	12.516930992029495
4	24.409920515186688	23.706251968399886	38.17935949644201	13.704468019971413
5	19.171499111329393	27.81505960810397	38.92443326601314	14.089008014553494
6	17.31399034024962	35.127486240471846	37.340243013419254	10.21828040585928
7	74.20432596481878	4.64795649808721	18.94729423476321	2.200423302330806
8	75.87022546024768	3.205821398131039	18.417175238795814	2.506777902825466
9	73.32028041026409	3.986353895724911	19.586432301359544	3.106933392651454
10	46.290158110688495	20.23878480075939	23.883765849060342	9.587291239491773
11	41.49574164902907	17.559999030941594	28.63016987153369	12.314089448495647
12	36.58217505159135	17.525861746199197	34.38285295199417	11.509110250215285
13	19.273690725009857	35.993251829648344	32.731929813742575	12.001127631599234
14	12.354833949639797	39.26096082140914	36.02166286017589	12.362542368775175
15	11.047926545372857	20.528621360249666	55.71557254833729	12.707879546040186
16	12.590711575182414	14.34624897312845	54.223442844274516	18.83959660741462
17	12.558556455360545	14.227539318443604	37.50718534783691	35.70671887835894
18	18.665386334955038	19.890584496386953	42.28970881997318	19.154320348684834
19	25.993890527233845	21.35055908062786	33.52942083343427	19.126129558704015
20	26.546253818420478	18.390305892095345	33.38802640243674	21.675413887047434
21	20.26323150144588	24.086827633140917	36.367440518534345	19.282500346878862
22	18.516063244275397	20.386566207611953	33.071981217886176	28.025389330226474
23	16.042101182911978	26.492735365566272	33.19003015093085	24.275133300590905
24	16.538523375230426	20.4237868600085	46.25580058539938	16.7818891793617
25	15.936605961030637	20.777713418595788	42.795160874707356	20.490519745666216
26	13.595669189889199	28.279106440053823	35.42304905417697	22.702175315880005
27	13.994304579461689	28.73786749888228	37.88203475836308	19.38579316329295
28	13.040222531048412	23.30056888133219	47.593101185114385	16.06610740250502
29	13.12259249552361	20.72617713066211	45.85716519582688	20.2940651779874
30	17.042653986684257	25.26291215265313	40.90703866763279	16.787395193029827
31	27.299916969313887	23.222603727791494	32.48746280687767	16.99001649601695
32	26.50727124165013	23.710216298240937	33.5331649227286	16.249347537380327
33	24.525106321123932	23.31598571960295	32.41588462919201	19.743023330081115
34	18.003563492046013	24.03088653427273	33.05634413906869	24.909205834612564
35	19.06402172452753	22.68213342612802	35.668617263775495	22.58522758556896
36	29.985309955533435	22.30640305341494	30.23043768403851	17.47784930701312
37	18.952800248431338	29.984208752799805	31.682483608597312	19.380507390171545
38	20.035062295038642	30.472922525982877	28.240564344376928	21.251450834601552
39	20.184165145171555	27.851399298313616	29.811319923620577	22.153115632894245
40	23.097286856704894	23.104995275840274	31.458719213124574	22.338998654330258
41	17.31575226462342	21.799409314853683	32.31963951027312	28.565198910249773
42	22.297152950452485	24.03507110466051	27.26115463309026	26.406621311796748
43	21.250349631867927	24.180209624952372	28.31412468698312	26.255316056196577
44	18.806780765952574	27.115795872251674	32.03156487515665	22.04585848663911
45	17.921634008664263	34.04874804261214	26.509253406570654	21.52036454215294
46	22.057310995068814	29.441315805122354	28.715402963116315	19.785970236692517
47	21.2448436181998	25.03760607335332	29.74018222702836	23.977368081418525
48	21.06975238355332	23.75272272375889	32.35487799774914	22.822646894938654
49	19.445918832548912	23.981993132899753	34.55684298390702	22.015245050644314
50	18.544254034256213	28.84512464513742	31.96901655988671	20.64160476071966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	446.0
2	641.0
3	6855.5
4	13070.0
5	9572.5
6	6075.0
7	6459.0
8	6843.0
9	7382.5
10	7922.0
11	7873.0
12	7824.0
13	7266.0
14	6708.0
15	6187.0
16	5666.0
17	5172.0
18	4678.0
19	4189.5
20	3701.0
21	3437.5
22	3174.0
23	3034.0
24	2894.0
25	2657.0
26	2420.0
27	2601.5
28	2783.0
29	3310.0
30	3837.0
31	4783.5
32	5730.0
33	6308.0
34	6886.0
35	8212.0
36	9538.0
37	9438.0
38	9338.0
39	12774.0
40	16210.0
41	22357.0
42	28504.0
43	35685.5
44	42867.0
45	41986.5
46	41106.0
47	40664.0
48	40222.0
49	40350.0
50	40478.0
51	37071.0
52	33664.0
53	28939.5
54	24215.0
55	20646.0
56	17077.0
57	15407.0
58	13737.0
59	13165.5
60	12594.0
61	11329.5
62	10065.0
63	8799.0
64	7533.0
65	6442.5
66	5352.0
67	4620.5
68	3889.0
69	3176.0
70	2463.0
71	2169.0
72	1875.0
73	1526.5
74	1178.0
75	852.5
76	527.0
77	410.0
78	293.0
79	204.5
80	116.0
81	80.5
82	45.0
83	40.5
84	36.0
85	24.5
86	13.0
87	8.0
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	454049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.271203435977576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21373400713695	25.240513216317538
2	7.563232818029256	4.881492498085682
3	3.1550920946247194	3.054558565346368
4	1.860172724434597	2.401200496651421
5	1.0895913662865206	1.7581212321758533
6	0.8228456399294839	1.5932531425542917
7	0.5922334624473381	1.3378460583762004
8	0.48423773370996176	1.2501547532744728
9	0.39918724371771996	1.159402747595553
>10	5.102915114056517	38.471436123002945
>50	0.6551453776647307	13.534404892956086
>100	0.058189505853189244	2.8638246678646126
>500	0.002738329687208906	0.5494354662484988
>1k	0.0	0.0
>5k	6.845824218022265E-4	1.9043561395504867
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8620	1.898473512770648	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	756	0.1665018533242007	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	610	0.13434673350233123	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	562	0.12377518725952485	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	559	0.12311446561934945	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0510958068402309	0.0
2	0.0	0.0	0.0	0.17949604558098356	0.0
3	0.0	0.0	0.0	0.2429253230378219	0.0
4	0.0	0.0	0.0	0.36163497772266867	0.0
5	0.0	0.0	0.0	0.5959709194382105	0.0
6	0.0	0.0	0.0	0.8512297130926397	0.0
7	0.0	0.0	0.0	0.9904217386229239	0.0
8	0.0	0.0	0.0	1.4002893960783969	0.0
9	0.0	0.0	0.0	1.5918986717292627	0.0
10	0.0	0.0	0.0	1.9319500758728683	0.0
11	0.0	0.0	0.0	2.1885303128076483	0.0
12	0.0	0.0	0.0	2.515367284147746	0.0
13	0.0	0.0	0.0	2.8479305097026972	0.0
14	0.0	0.0	0.0	3.0386588231666627	0.0
15	0.0	0.0	0.0	3.1280764851370666	0.0
16	2.202405467251332E-4	0.0	0.0	3.247226620915364	0.0
17	2.202405467251332E-4	0.0	0.0	3.38135311387097	0.0
18	2.202405467251332E-4	0.0	0.0	3.644320326660779	0.0
19	4.404810934502664E-4	0.0	0.0	3.708850806851243	0.0
20	4.404810934502664E-4	0.0	0.0	3.808399533971003	0.0
21	4.404810934502664E-4	0.0	0.0	3.8978171959414074	0.0
22	4.404810934502664E-4	0.0	0.0	3.9795264387764315	0.0
23	4.404810934502664E-4	0.0	0.0	4.0843609390175954	0.0
24	4.404810934502664E-4	0.0	0.0	4.1594629654508655	0.0
25	4.404810934502664E-4	0.0	0.0	4.219368394160101	0.0
26	4.404810934502664E-4	0.0	0.0	4.276190455215186	0.0
27	4.404810934502664E-4	0.0	0.0	4.349750797821381	0.0
28	4.404810934502664E-4	0.0	0.0	4.412078872544593	0.0
29	4.404810934502664E-4	0.0	0.0	4.479252239295759	0.0
30	4.404810934502664E-4	0.0	0.0	4.574396155481017	0.0
31	6.607216401753995E-4	0.0	0.0	4.6442124087928836	0.0
32	6.607216401753995E-4	0.0	0.0	4.719754916319604	0.0
33	6.607216401753995E-4	0.0	0.0	4.820184605626265	0.0
34	8.809621869005328E-4	0.0	0.0	4.933388246642983	0.0
35	8.809621869005328E-4	0.0	0.0	5.066193296318239	0.0
36	8.809621869005328E-4	0.0	0.0	5.146140614779462	0.0
37	8.809621869005328E-4	0.0	0.0	5.223665287226709	0.0
38	8.809621869005328E-4	0.0	0.0	5.300749478580506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATT	20	7.851443E-4	44.000004	34
TCTTGAG	20	7.851443E-4	44.000004	1
CGACGGT	65	0.0	44.000004	28
ACGTTAG	20	7.851443E-4	44.000004	1
GGCGTAG	20	7.851443E-4	44.000004	1
TTCACGA	20	7.851443E-4	44.000004	11
CACGATA	20	7.851443E-4	44.000004	13
GCTTGTA	20	7.851443E-4	44.000004	42
AGGCGAG	20	7.851443E-4	44.000004	1
AACGGGA	80	0.0	44.000004	4
GTTACAC	20	7.851443E-4	44.000004	33
GTTAAGC	20	7.851443E-4	44.000004	37
CTACGTC	20	7.851443E-4	44.000004	30
ACTACGG	20	7.851443E-4	44.000004	2
GCTACGA	20	7.851443E-4	44.000004	10
CACGTGC	20	7.851443E-4	44.000004	41
AACTACG	20	7.851443E-4	44.000004	1
TAAGCTT	20	7.851443E-4	44.000004	39
TGTAACG	20	7.851443E-4	44.000004	1
TACGTCC	20	7.851443E-4	44.000004	31
>>END_MODULE