FastQCFastQC Report
Thu 26 May 2016
SRR1546087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546087_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448196
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86031.9194727306803272No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7400.16510633740595632No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6740.15038063704272236No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6030.13453935331863737No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5720.12762273648136083No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT207.851334E-444.00000437
ACTCTTG207.851334E-444.0000041
CAACCCG207.851334E-444.00000423
ATGTACC207.851334E-444.00000438
AACGGTT207.851334E-444.00000427
GTTACGG207.851334E-444.0000042
AAACGAG207.851334E-444.0000041
CGCTCCA207.851334E-444.00000415
TAACAGT207.851334E-444.00000435
GTACTAC207.851334E-444.00000432
TGTAGCA207.851334E-444.00000422
CGTCGCA207.851334E-444.00000421
TTGCACG408.292773E-944.0000041
ATACGAG207.851334E-444.0000041
TGTAACG207.851334E-444.0000041
AGTACAG207.851334E-444.0000041
GTTGCAT207.851334E-444.00000442
CGGACCC207.851334E-444.00000415
GTACATC254.4384804E-544.036
TTGTGCG302.524248E-644.01
Produced by FastQC (version 0.11.5)