FastQCFastQC Report
Thu 26 May 2016
SRR1546086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546086_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences382042
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41361.0826034833866434No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT4610.12066736118018437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACG650.044.0000041
CTAGCGG650.044.0000042
GCGAGAA207.8498747E-444.028
CCTTATG207.8498747E-444.032
ACAGTCT254.4372435E-544.024
CTCGTAG351.442695E-744.01
TTGCTCA254.4372435E-544.028
CGTTGGC207.8498747E-444.03
TAGCGGC207.8498747E-444.03
GGGTACA207.8498747E-444.07
AAGACGG302.523264E-644.02
CACGACG502.7284841E-1144.026
TGCGGGT302.523264E-644.04
AGGCGAT351.442695E-744.06
GCGTTCG207.8498747E-444.044
CCCACGT207.8498747E-444.023
GACCATC207.8498747E-444.09
ATCCGGG207.8498747E-444.03
CCGTAGG351.442695E-744.02
GCACACG454.783942E-1044.024
Produced by FastQC (version 0.11.5)