##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546086_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	382042
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.15400401003031	28.0	25.0	30.0	16.0	33.0
2	26.148436559331174	30.0	25.0	31.0	10.0	33.0
3	26.507946770250392	30.0	25.0	31.0	16.0	33.0
4	27.983300265415846	32.0	19.0	35.0	10.0	37.0
5	31.322333670120038	33.0	30.0	35.0	19.0	37.0
6	29.930177833850728	33.0	28.0	35.0	17.0	37.0
7	29.789491731275618	32.0	28.0	35.0	17.0	35.0
8	32.48983881353359	33.0	32.0	35.0	28.0	35.0
9	27.180477539118737	31.0	23.0	34.0	10.0	35.0
10	31.96854796069542	35.0	31.0	37.0	18.0	39.0
11	33.92629867920281	35.0	32.0	37.0	27.0	39.0
12	33.26039283638972	35.0	31.0	38.0	25.0	39.0
13	32.93801990357081	35.0	31.0	38.0	22.0	39.0
14	32.59342951822051	35.0	30.0	38.0	21.0	40.0
15	31.87862067521372	34.0	27.0	38.0	18.0	40.0
16	30.58383894964428	33.0	27.0	37.0	17.0	40.0
17	30.302278283539508	32.0	25.0	37.0	17.0	40.0
18	31.414619335046932	34.0	28.0	37.0	18.0	39.0
19	31.022526319095807	34.0	27.0	37.0	18.0	39.0
20	31.43470089676004	34.0	29.0	36.0	19.0	39.0
21	32.42203736761927	34.0	31.0	36.0	23.0	39.0
22	32.76549698724224	34.0	31.0	37.0	25.0	39.0
23	34.19852005800409	35.0	33.0	38.0	29.0	39.0
24	33.884274503850364	35.0	33.0	38.0	27.0	40.0
25	32.66500280073919	35.0	31.0	37.0	23.0	39.0
26	26.31175891655891	33.0	21.0	35.0	0.0	38.0
27	29.51754780887965	34.0	24.0	36.0	12.0	39.0
28	30.98898288669832	34.0	27.0	36.0	18.0	39.0
29	32.30641657199994	34.0	30.0	37.0	23.0	39.0
30	31.996123462865338	34.0	30.0	37.0	21.0	39.0
31	30.952520927018494	34.0	29.0	36.0	18.0	38.0
32	30.97589270289654	34.0	29.0	36.0	18.0	39.0
33	30.60178723805236	33.0	29.0	36.0	16.0	38.0
34	30.599206369980266	34.0	29.0	37.0	15.0	39.0
35	29.725090435083054	33.0	27.0	36.0	10.0	39.0
36	29.34598551991666	33.0	25.0	36.0	10.0	39.0
37	29.391135006098807	33.0	25.0	36.0	10.0	39.0
38	29.214154464692363	33.0	26.0	36.0	10.0	38.0
39	29.122873924856428	33.0	25.0	36.0	9.0	39.0
40	29.03227132095424	33.0	25.0	36.0	10.0	38.0
41	28.54602635312348	33.0	23.0	35.0	8.0	38.0
42	28.692654210793577	33.0	24.0	35.0	8.0	38.0
43	28.326338465404326	32.0	23.0	35.0	8.0	38.0
44	27.961169190821952	32.0	23.0	35.0	8.0	38.0
45	27.24563267912952	31.0	23.0	34.0	8.0	37.0
46	26.885769103920513	31.0	22.0	34.0	7.0	37.0
47	27.127368195119907	31.0	23.0	34.0	7.0	37.0
48	27.2853403552489	31.0	23.0	34.0	8.0	37.0
49	27.004337219468017	31.0	22.0	34.0	7.0	37.0
50	28.752548149156375	33.0	24.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	8.0
12	11.0
13	45.0
14	116.0
15	217.0
16	482.0
17	1008.0
18	1661.0
19	2818.0
20	4225.0
21	5958.0
22	8177.0
23	10950.0
24	14052.0
25	17864.0
26	20705.0
27	22633.0
28	24513.0
29	28565.0
30	33462.0
31	38853.0
32	41283.0
33	38601.0
34	31004.0
35	22227.0
36	10585.0
37	1966.0
38	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.34287329665325	22.938053931243164	26.275383334816592	26.443689437287
2	39.88252600499422	17.547547128326205	29.661136733657557	12.908790133022025
3	28.536391286821868	13.96495673250585	43.754089864465165	13.744562116207119
4	25.96023473858895	23.743462760638884	36.63654781411468	13.659754686657488
5	18.56366577496715	28.107642615209844	39.322639919171195	14.006051690651814
6	17.30856816789777	34.03657189523665	37.99372843823454	10.661131498631041
7	76.81380581192644	3.406955256228373	18.30662597306055	1.4726129587846364
8	79.19024609859649	1.7806942692164736	17.486559069421688	1.5425005627653503
9	68.16868302437952	2.3081231906439603	17.3703414807796	12.152852304196921
10	48.1601499311594	19.9590620926495	23.440092974070915	8.440695002120185
11	43.473754194565	16.54975107448919	28.300029839651135	11.676464891294675
12	37.96336528444517	17.704859675114253	34.05986776323022	10.271907277210358
13	18.46734128708362	37.50268295108914	32.453238125651104	11.576737636176128
14	11.57307311761534	39.686212510666365	36.476617754068926	12.264096617649368
15	9.745001858434414	19.030106637490118	55.89097533779008	15.333916166285382
16	11.187513414755445	12.174315912910098	54.6031588149994	22.035011857335057
17	11.80419953827066	13.189387554247961	37.93954591380005	37.06686699368132
18	17.953523434596196	19.61407384528403	43.43239748509326	19.000005235026514
19	26.69313845074625	20.83619078530633	33.901508211139095	18.56916255280833
20	27.668162139241236	17.93860360902728	33.504431449945294	20.88880280178619
21	19.73369420116113	24.237911015019293	36.49415509289555	19.534239690924036
22	18.255322713209544	19.775050910632864	32.740902832672845	29.228723543484747
23	15.2477999801069	26.959077797729048	33.622219546542006	24.170902675622052
24	15.735966202668816	19.830803943021973	48.588375100119876	15.844854754189331
25	15.634668439595648	19.539212966113674	44.53227655598076	20.293842038309922
26	10.657466980070254	40.704163416587704	29.19024609859649	19.448123504745553
27	12.869003931504913	30.19667994618393	38.16726956722036	18.7670465550908
28	12.007318567068543	23.356594301150135	48.87054302930044	15.765544102480881
29	12.268284638861696	20.829647002162066	46.54959402369373	20.35247433528251
30	16.44217127959753	25.663146983839475	41.199396924945425	16.69528481161757
31	28.59423832981714	22.66112102857801	32.718130467330816	16.026510174274033
32	27.396987765743035	22.73545840509682	33.4520811847912	16.415472644368943
33	25.213719957491588	22.89879123237759	31.965071903089186	19.922416907041633
34	17.3357903057779	23.506577810816612	32.62494699535653	26.53268488804896
35	19.061778547908347	21.927693813769167	35.69476654399254	23.315761094329943
36	30.622811104538243	21.77640154747384	29.55565094937206	18.04513639861586
37	18.38384261416284	30.8172923395857	30.805251778600258	19.9936132676512
38	19.25050125378885	31.683950979211712	27.812125368414993	21.253422398584448
39	19.264374074054686	28.145858308772333	29.558268462629762	23.031499154543216
40	23.09065495416734	22.570293318535658	31.321425393019613	23.017626334277384
41	16.742661801582027	20.806612885494268	32.37104820935918	30.079677103564528
42	21.272006742714154	23.114736076138225	26.324069081409895	29.28918809973772
43	20.761591657461747	22.918684333136145	28.13486475308997	28.184859256312134
44	17.974987043309373	26.620109830856293	32.58673130179404	22.81817182404029
45	17.318514718277047	35.52384292826443	25.670476020961047	21.487166332497473
46	21.308390176996248	29.424775286486827	28.007130106113987	21.25970443040294
47	20.739081043445484	24.700425607655703	28.99969113343559	25.56080221546322
48	20.14412027996922	23.298223755503322	33.262049722281844	23.29560624224562
49	18.747676956983785	24.16331188717471	34.41297030169458	22.676040854146926
50	18.015558498803795	29.958747991058576	31.430052193214358	20.59564131692327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	226.5
2	305.0
3	3687.5
4	7070.0
5	5731.0
6	4392.0
7	4654.0
8	4916.0
9	5436.5
10	5957.0
11	6252.0
12	6547.0
13	6577.5
14	6608.0
15	6282.0
16	5956.0
17	5517.0
18	5078.0
19	4731.5
20	4385.0
21	4009.0
22	3633.0
23	3354.0
24	3075.0
25	2797.0
26	2519.0
27	2613.5
28	2708.0
29	3099.0
30	3490.0
31	4307.5
32	5125.0
33	5668.0
34	6211.0
35	6957.0
36	7703.0
37	7722.0
38	7741.0
39	10330.0
40	12919.0
41	18333.5
42	23748.0
43	29487.5
44	35227.0
45	35292.5
46	35358.0
47	35149.5
48	34941.0
49	33965.0
50	32989.0
51	30251.5
52	27514.0
53	23973.0
54	20432.0
55	17546.5
56	14661.0
57	13222.0
58	11783.0
59	11111.0
60	10439.0
61	9425.0
62	8411.0
63	7321.0
64	6231.0
65	5465.5
66	4700.0
67	4017.0
68	3334.0
69	2767.0
70	2200.0
71	1919.5
72	1639.0
73	1308.5
74	978.0
75	719.5
76	461.0
77	357.0
78	253.0
79	187.5
80	122.0
81	96.5
82	71.0
83	54.5
84	38.0
85	24.5
86	11.0
87	8.5
88	6.0
89	6.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	382042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.547166545376854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.18311724798409	40.02668696098886
2	6.084824356330344	5.786323141396089
3	2.3004302098563687	3.2813681494217093
4	1.294363144311386	2.4617319997108456
5	0.8348280403840601	1.9846853936445752
6	0.6574371070787886	1.8755562974031554
7	0.47735237573022443	1.5887727036773405
8	0.37284876732193806	1.4182321948875722
9	0.32707996801848516	1.3996553141728307
>10	3.2661676492952068	32.11577112740015
>50	0.18127675929439843	5.416200863482064
>100	0.019678069265510356	1.472351519869254
>500	0.0	0.0
>1k	5.963051292578895E-4	1.172664333945549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4136	1.0826034833866434	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	461	0.12066736118018437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034551175001701386	0.0
2	0.0	0.0	0.0	0.14841300171185368	0.0
3	0.0	0.0	0.0	0.21489783845755178	0.0
4	0.0	0.0	0.0	0.30101402463603477	0.0
5	0.0	0.0	0.0	0.4648703545683459	0.0
6	0.0	0.0	0.0	0.6216593987048544	0.0
7	0.0	0.0	0.0	0.7140576167018285	0.0
8	0.0	0.0	0.0	0.9721444239115071	0.0
9	0.0	0.0	0.0	1.1346919972149658	0.0
10	0.0	0.0	0.0	1.3794294868103507	0.0
11	0.0	0.0	0.0	1.5838572722370838	0.0
12	0.0	0.0	0.0	1.8432528360756146	0.0
13	0.0	0.0	0.0	2.1442668607116495	0.0
14	0.0	0.0	0.0	2.3070761853408785	0.0
15	0.0	0.0	0.0	2.3772255406473635	0.0
16	0.0	0.0	0.0	2.449468906560012	0.0
17	0.0	0.0	0.0	2.5539076855424274	0.0
18	0.0	0.0	0.0	2.740536380816769	0.0
19	0.0	0.0	0.0	2.777705069076175	0.0
20	0.0	0.0	0.0	2.8434046518445615	0.0
21	0.0	0.0	0.0	2.892090398437868	0.0
22	0.0	0.0	0.0	2.9452259175692723	0.0
23	0.0	0.0	0.0	3.0035964632160863	0.0
24	0.0	0.0	0.0	3.042859162081656	0.0
25	0.0	0.0	0.0	3.0747928238256526	0.0
26	0.0	0.0	0.0	3.105417728940797	0.0
27	0.0	0.0	0.0	3.151747713602169	0.0
28	0.0	0.0	0.0	3.2035744761047216	0.0
29	0.0	0.0	0.0	3.256709995236126	0.0
30	0.0	0.0	0.0	3.321362572701431	0.0
31	0.0	0.0	0.0	3.3710953245978192	0.0
32	0.0	0.0	0.0	3.4297276215704033	0.0
33	0.0	0.0	0.0	3.502232738808822	0.0
34	0.0	0.0	0.0	3.5948927081315665	0.0
35	0.0	0.0	0.0	3.7066605242355553	0.0
36	0.0	0.0	0.0	3.7652928212081394	0.0
37	0.0	0.0	0.0	3.8252338748095758	0.0
38	0.0	0.0	0.0	3.886745436365635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGACG	65	0.0	44.000004	1
CTAGCGG	65	0.0	44.000004	2
GCGAGAA	20	7.8498747E-4	44.0	28
CCTTATG	20	7.8498747E-4	44.0	32
ACAGTCT	25	4.4372435E-5	44.0	24
CTCGTAG	35	1.442695E-7	44.0	1
TTGCTCA	25	4.4372435E-5	44.0	28
CGTTGGC	20	7.8498747E-4	44.0	3
TAGCGGC	20	7.8498747E-4	44.0	3
GGGTACA	20	7.8498747E-4	44.0	7
AAGACGG	30	2.523264E-6	44.0	2
CACGACG	50	2.7284841E-11	44.0	26
TGCGGGT	30	2.523264E-6	44.0	4
AGGCGAT	35	1.442695E-7	44.0	6
GCGTTCG	20	7.8498747E-4	44.0	44
CCCACGT	20	7.8498747E-4	44.0	23
GACCATC	20	7.8498747E-4	44.0	9
ATCCGGG	20	7.8498747E-4	44.0	3
CCGTAGG	35	1.442695E-7	44.0	2
GCACACG	45	4.783942E-10	44.0	24
>>END_MODULE