FastQCFastQC Report
Thu 26 May 2016
SRR1546085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546085_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences809826
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147601.8226137466566892No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13370.16509719371815673No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT11880.14669817960895304No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10790.13323849814651542No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT10000.1234833161691524No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT254.4416716E-544.014
GCGTTAG302.5267855E-644.01
GTCCGAC207.8551035E-444.025
TCGTAAG254.4416716E-544.01
CGGTCTA1700.044.031
ATCGATC207.8551035E-444.010
CGTTTTT93100.043.503761
TCACGAC1700.041.41176625
CGACGGT1650.041.33333228
TAGCCGT603.6379788E-1240.33333244
GTACGAG603.6379788E-1240.3333321
CTCACGA1750.040.2285724
TTCGAAT557.8216544E-1140.00000416
TATCGGG501.3460522E-939.63
CATATGC6950.039.56834833
ATATGCT6900.039.53623234
TAGGGTA4300.039.395355
GTTTTTT106900.039.1431242
GTTGATC7200.038.80555716
AGCATAT7100.038.73239531
Produced by FastQC (version 0.11.5)