##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546085_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	809826
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.849156485467248	31.0	28.0	31.0	16.0	33.0
2	28.7527011975412	31.0	28.0	33.0	16.0	33.0
3	28.261105472039674	31.0	27.0	33.0	16.0	34.0
4	32.265865753877996	35.0	32.0	35.0	26.0	37.0
5	33.669775976567806	35.0	33.0	35.0	28.0	37.0
6	33.44522033128104	35.0	33.0	37.0	28.0	37.0
7	34.338015820682465	35.0	35.0	37.0	31.0	37.0
8	34.71295191806635	35.0	35.0	37.0	32.0	37.0
9	36.42987629441386	39.0	37.0	39.0	32.0	39.0
10	36.164715630271196	38.0	35.0	39.0	31.0	39.0
11	36.283336173449605	38.0	35.0	39.0	31.0	39.0
12	35.110200215848835	37.0	34.0	39.0	29.0	39.0
13	35.2368743409078	37.0	34.0	39.0	27.0	39.0
14	35.98556480033982	38.0	34.0	40.0	29.0	41.0
15	36.55061210679825	38.0	35.0	40.0	31.0	41.0
16	36.189060366053944	38.0	34.0	40.0	31.0	41.0
17	35.68160444342365	37.0	34.0	40.0	29.0	41.0
18	35.402882841499284	37.0	34.0	39.0	29.0	40.0
19	34.870785823127434	37.0	33.0	39.0	27.0	40.0
20	35.21043532808282	35.0	34.0	39.0	29.0	40.0
21	35.46612729154164	36.0	34.0	39.0	30.0	40.0
22	35.79596481219423	36.0	34.0	40.0	31.0	41.0
23	36.128814090928174	37.0	35.0	40.0	31.0	41.0
24	35.857838597427104	36.0	35.0	40.0	31.0	41.0
25	35.49088322676723	36.0	34.0	40.0	30.0	41.0
26	35.59315704855117	36.0	34.0	40.0	30.0	41.0
27	35.86593292880199	37.0	35.0	40.0	31.0	41.0
28	35.802031547517615	36.0	35.0	40.0	31.0	41.0
29	35.914022271450904	37.0	35.0	40.0	31.0	41.0
30	35.53638435910924	36.0	35.0	40.0	30.0	41.0
31	34.853988387628945	36.0	34.0	40.0	28.0	41.0
32	34.78516372652891	36.0	34.0	40.0	27.0	41.0
33	34.45824041213792	36.0	34.0	40.0	24.0	41.0
34	34.120569109907564	36.0	34.0	40.0	22.0	41.0
35	33.86172214772062	36.0	33.0	40.0	20.0	41.0
36	33.45517802589692	36.0	33.0	40.0	18.0	41.0
37	33.39124453895034	36.0	33.0	40.0	18.0	41.0
38	33.20721982252978	36.0	33.0	40.0	18.0	41.0
39	33.02850488870449	35.0	33.0	40.0	16.0	40.0
40	32.82867307298111	35.0	32.0	40.0	15.0	40.0
41	32.5298101073564	35.0	32.0	39.0	15.0	40.0
42	32.71380642261424	35.0	32.0	39.0	15.0	40.0
43	32.52388908234608	35.0	32.0	39.0	15.0	40.0
44	32.384864156003886	35.0	32.0	39.0	13.0	40.0
45	32.277757197225085	35.0	31.0	39.0	13.0	40.0
46	32.09268904678289	35.0	31.0	39.0	12.0	40.0
47	32.04514426555828	35.0	31.0	38.0	12.0	40.0
48	32.05615527286108	35.0	31.0	38.0	12.0	40.0
49	32.026136478700366	35.0	31.0	38.0	12.0	40.0
50	32.19053105234952	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	6.0
12	8.0
13	24.0
14	45.0
15	90.0
16	220.0
17	471.0
18	973.0
19	1664.0
20	2573.0
21	3788.0
22	5399.0
23	7551.0
24	10857.0
25	16361.0
26	22544.0
27	25657.0
28	24187.0
29	23867.0
30	25761.0
31	31394.0
32	41856.0
33	63363.0
34	98340.0
35	76195.0
36	78691.0
37	106841.0
38	116133.0
39	24961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.350872903562	22.179455833722304	25.59710357533594	22.872567687379757
2	39.54269188689916	20.33189845719945	27.582591816019736	12.542817839881653
3	29.518686730235878	18.59201853237609	38.889835594312856	12.999459143075178
4	25.533139217560315	24.22162291652775	35.60950130027932	14.635736565632618
5	20.30658437738477	28.27064085371426	36.68034861809821	14.742426150802764
6	18.453099801685795	36.736163077006665	34.40936695043133	10.401370170876213
7	75.22813542662252	5.114061539145446	17.076260826399743	2.5815422078323
8	76.46370948821105	3.859841496815365	16.646415402814927	3.030033612158661
9	72.83404089273499	4.8536352253447035	17.940644039583812	4.371679842336502
10	42.27735340678121	22.29849375050937	23.604453302314322	11.819699540395098
11	37.14983218617333	20.368696485417857	28.70419077678415	13.77728055162467
12	33.65809445485821	19.968116607765126	32.73678543292016	13.637003504456512
13	20.510331849063874	31.758180152279625	33.56128353498159	14.170204463674912
14	15.480732898178125	34.877492202028584	35.16928327813629	14.472491621656996
15	14.922958759042066	22.016210889746688	48.1105817792958	14.950248571915449
16	16.813858779540297	17.37718966790397	47.99191431245725	17.81703724009849
17	16.591341843803484	17.713064287884066	35.687789722730564	30.00780414558189
18	19.95576827614821	21.99237860972604	39.66148283705388	18.390370277071867
19	25.37532753949614	23.508630248967062	32.186790742702755	18.929251468834046
20	26.35726686967324	21.80307868603873	32.31262024187912	19.527034202408913
21	21.76097087522505	25.223319577291914	34.70733219234749	18.308377355135548
22	20.2716385989089	21.57130050158923	32.555264958151504	25.601795941350364
23	18.239103214764654	27.375757261436405	32.5700829560918	21.815056567707135
24	18.082773336494505	23.16695191312702	42.19992936754315	16.550345382835324
25	19.0743443653328	22.22650297718275	39.25423486032802	19.444917797156428
26	16.00170900909578	27.99107462590729	34.16141245156367	21.845803913433258
27	16.527007036079354	28.053680667205054	35.89783484353429	19.5214774531813
28	15.125841847507985	25.377797205819526	42.242654594937676	17.253706351734817
29	16.646168436182588	24.08739655185188	39.74285834240936	19.523576669556174
30	18.37246519622734	26.08770279047598	37.80034229575242	17.739489717544263
31	26.886269396141888	23.617666017144423	31.05531311664481	18.44075147006888
32	26.231931303761545	24.314111920338444	31.56406437926172	17.88989239663829
33	24.682956585735703	23.55975234186109	30.225134782039596	21.53215629036361
34	17.937063023414908	25.366436740731963	32.080224640848776	24.616275595004357
35	19.34674856080195	23.9227932913984	33.845789095435315	22.884669052364334
36	27.461578166173968	24.932886817662066	28.527980084610764	19.0775549315532
37	19.15744863711464	29.450523939710504	30.420855838167704	20.971171585007152
38	20.443527375016362	31.099890593781875	27.437498919520987	21.019083111680782
39	19.74387090560194	28.313242597792616	28.847554906856537	23.09533158974891
40	22.948880376772294	24.140864827753123	30.850454295120187	22.0598005003544
41	18.72179949766987	23.371687251335473	31.765095217985095	26.14141803300956
42	20.82113935586163	25.568331962668523	27.46528266565904	26.1452460158108
43	20.054060995818855	24.940295816632215	28.80544709604285	26.20019609150608
44	19.62816703835145	26.383568816017267	31.385630987397295	22.602633158233992
45	19.127812641234044	31.721876057325893	27.209548717872728	21.940762583567334
46	22.340725044639218	28.446851545887636	28.43437973095455	20.778043678518596
47	20.929681190774314	26.430122026213038	29.449783039813493	23.190413743199155
48	20.308436627127307	25.945820460197623	32.06725889265101	21.678484020024055
49	20.343999822184024	25.54820418213295	32.50303151541195	21.604764480271072
50	19.368234657815382	28.08615677935754	31.612469839200024	20.93313872362705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	568.0
1	825.5
2	1083.0
3	11710.0
4	22337.0
5	15760.0
6	9183.0
7	9289.0
8	9395.0
9	9832.5
10	10270.0
11	10271.5
12	10273.0
13	9770.0
14	9267.0
15	8720.0
16	8173.0
17	7584.5
18	6996.0
19	6431.5
20	5867.0
21	5629.5
22	5392.0
23	5194.5
24	4997.0
25	5359.0
26	5721.0
27	6372.0
28	7023.0
29	8025.5
30	9028.0
31	10095.5
32	11163.0
33	13582.0
34	16001.0
35	17830.0
36	19659.0
37	21247.5
38	22836.0
39	27862.5
40	32889.0
41	42723.5
42	52558.0
43	60175.5
44	67793.0
45	71042.5
46	74292.0
47	71562.5
48	68833.0
49	68489.0
50	68145.0
51	61614.5
52	55084.0
53	48232.0
54	41380.0
55	37681.5
56	33983.0
57	30951.5
58	27920.0
59	26141.0
60	24362.0
61	21937.5
62	19513.0
63	17460.0
64	15407.0
65	12807.5
66	10208.0
67	8587.0
68	6966.0
69	6350.0
70	5734.0
71	4954.0
72	4174.0
73	3398.0
74	2622.0
75	1976.0
76	1330.0
77	965.0
78	600.0
79	455.5
80	311.0
81	266.5
82	222.0
83	166.0
84	110.0
85	89.0
86	68.0
87	48.5
88	29.0
89	27.0
90	25.0
91	19.0
92	13.0
93	12.5
94	12.0
95	10.5
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	809826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.166366085880693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.60156242220447	23.562259711149856
2	7.499058552763745	4.674368083097791
3	3.4775857929721528	3.2515113575648344
4	2.2018273284866687	2.744918263100488
5	1.528061102260331	2.3812055857319927
6	1.141620347888985	2.134809461603915
7	0.8946638696842744	1.9518395180473522
8	0.7084731168703489	1.7664426017908983
9	0.5821815564533086	1.633003516518447
>10	5.750489140669389	39.080368428499426
>50	0.5575660329723596	11.109073030141765
>100	0.05293096497159602	2.9269785183242027
>500	0.0023878630814253843	0.5054427265942506
>1k	0.0011939315407126921	0.44702222985170037
>5k	0.0	0.0
>10k+	3.9797718023756407E-4	1.8307569679831017
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14760	1.8226137466566892	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1337	0.16509719371815673	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1188	0.14669817960895304	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1079	0.13323849814651542	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	1000	0.1234833161691524	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2348331616915238E-4	0.0	0.0	0.03827982801243724	0.0
2	1.2348331616915238E-4	0.0	0.0	0.14583379639576896	0.0
3	1.2348331616915238E-4	0.0	0.0	0.1984376890838279	0.0
4	1.2348331616915238E-4	0.0	0.0	0.3226619051499952	0.0
5	1.2348331616915238E-4	0.0	0.0	0.6610061914534727	0.0
6	1.2348331616915238E-4	0.0	0.0	0.9234082383129215	0.0
7	1.2348331616915238E-4	0.0	0.0	1.1128316453164013	0.0
8	1.2348331616915238E-4	0.0	0.0	1.5657684490248522	0.0
9	1.2348331616915238E-4	0.0	0.0	1.7666758044320632	0.0
10	1.2348331616915238E-4	0.0	0.0	2.1666382655039476	0.0
11	1.2348331616915238E-4	0.0	0.0	2.3870559848658846	0.0
12	3.7044994850745716E-4	0.0	0.0	2.6543973643720995	0.0
13	4.939332646766095E-4	0.0	0.0	2.7473803014474716	0.0
14	4.939332646766095E-4	0.0	0.0	2.785042712879063	0.0
15	6.17416580845762E-4	0.0	0.0	2.8403632385228432	0.0
16	6.17416580845762E-4	0.0	0.0	2.930012126061648	0.0
17	6.17416580845762E-4	0.0	0.0	3.0365782279156264	0.0
18	6.17416580845762E-4	0.0	0.0	3.219457019162141	0.0
19	6.17416580845762E-4	0.0	0.0	3.2902129593270653	0.0
20	6.17416580845762E-4	0.0	0.0	3.3683778984621386	0.0
21	6.17416580845762E-4	0.0	0.0	3.463460051912386	0.0
22	6.17416580845762E-4	0.0	0.0	3.566445137597459	0.0
23	6.17416580845762E-4	0.0	0.0	3.6859769876491986	0.0
24	6.17416580845762E-4	0.0	0.0	3.7725387922837745	0.0
25	7.408998970149143E-4	0.0	0.0	3.8395902329636242	0.0
26	0.0011113498455223715	0.0	0.0	3.909975723180041	0.0
27	0.0011113498455223715	0.0	0.0	3.9894989787929753	0.0
28	0.0011113498455223715	0.0	0.0	4.085445515456406	0.0
29	0.0011113498455223715	0.0	0.0	4.177811035950933	0.0
30	0.0011113498455223715	0.0	0.0	4.306480651399189	0.0
31	0.0011113498455223715	0.0	0.0	4.40403247117282	0.0
32	0.0011113498455223715	0.0	0.0	4.497756308145206	0.0
33	0.0011113498455223715	0.0	0.0	4.602593643572817	0.0
34	0.0011113498455223715	0.0	0.0	4.713358178176547	0.0
35	0.001234833161691524	0.0	0.0	4.8584510746753	0.0
36	0.0014817997940298287	0.0	0.0	4.955508961184254	0.0
37	0.0016052831101989811	0.0	0.0	5.063556862832263	0.0
38	0.0018522497425372858	0.0	0.0	5.197536260875793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	25	4.4416716E-5	44.0	14
GCGTTAG	30	2.5267855E-6	44.0	1
GTCCGAC	20	7.8551035E-4	44.0	25
TCGTAAG	25	4.4416716E-5	44.0	1
CGGTCTA	170	0.0	44.0	31
ATCGATC	20	7.8551035E-4	44.0	10
CGTTTTT	9310	0.0	43.50376	1
TCACGAC	170	0.0	41.411766	25
CGACGGT	165	0.0	41.333332	28
TAGCCGT	60	3.6379788E-12	40.333332	44
GTACGAG	60	3.6379788E-12	40.333332	1
CTCACGA	175	0.0	40.22857	24
TTCGAAT	55	7.8216544E-11	40.000004	16
TATCGGG	50	1.3460522E-9	39.6	3
CATATGC	695	0.0	39.568348	33
ATATGCT	690	0.0	39.536232	34
TAGGGTA	430	0.0	39.39535	5
GTTTTTT	10690	0.0	39.143124	2
GTTGATC	720	0.0	38.805557	16
AGCATAT	710	0.0	38.732395	31
>>END_MODULE