FastQCFastQC Report
Thu 26 May 2016
SRR1546084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546084_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences813353
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154001.8933968399944427No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12820.15761913953719972No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT11440.14065233668530144No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10940.13450494434765717No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT9900.12171836828535702No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC8270.10167786926463664No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA207.8551215E-444.00000430
GTTTGCG351.4453872E-744.01
TAAGTCG254.4416884E-544.013
AACTCCG302.5268018E-644.029
TGTCGGT254.4416884E-544.044
GCGTAAG302.5268018E-644.01
TACGTAG454.802132E-1044.01
CGTTTTT99900.042.810811
CGTAAGG1050.041.9047622
TCACGAC1450.040.96551525
CGGTCTA1350.040.7407431
GGTACCT7400.040.729738
ATGGGAC3800.040.5263145
ATCCTGC7450.040.4563820
GTTGATC7400.040.4324316
TCGTTCA1100.040.016
TGGGCGA2750.040.06
ATGGGCC1050.039.8095255
ATGGGCG1900.039.368425
GACGGGA1750.038.9714284
Produced by FastQC (version 0.11.5)