##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546084_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	813353
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.821637099758654	31.0	28.0	31.0	16.0	33.0
2	28.71797731120436	31.0	28.0	33.0	16.0	33.0
3	28.25953798658147	31.0	27.0	33.0	16.0	34.0
4	32.25288527859367	35.0	32.0	35.0	26.0	37.0
5	33.708762370090234	35.0	33.0	35.0	28.0	37.0
6	33.54175739193192	35.0	33.0	37.0	28.0	37.0
7	34.3960469808312	35.0	35.0	37.0	31.0	37.0
8	34.74664506063173	35.0	35.0	37.0	32.0	37.0
9	36.54078241550717	39.0	37.0	39.0	32.0	39.0
10	36.15974613728603	38.0	35.0	39.0	31.0	39.0
11	36.33316776356637	38.0	35.0	39.0	32.0	39.0
12	35.06811064814416	37.0	34.0	39.0	29.0	39.0
13	35.25384673075528	37.0	34.0	39.0	27.0	39.0
14	36.00382982542635	38.0	34.0	40.0	29.0	41.0
15	36.58515921131415	38.0	35.0	40.0	31.0	41.0
16	36.226974019890505	38.0	34.0	40.0	31.0	41.0
17	35.737415365775995	37.0	34.0	40.0	30.0	41.0
18	35.4841255887665	37.0	34.0	39.0	29.0	40.0
19	34.95381341188881	37.0	33.0	39.0	27.0	40.0
20	35.19608460287231	35.0	34.0	39.0	29.0	40.0
21	35.37913796346728	36.0	34.0	39.0	30.0	40.0
22	35.77689023093294	36.0	34.0	39.0	31.0	40.0
23	36.15917197084169	37.0	35.0	40.0	31.0	41.0
24	35.74741225519547	36.0	34.0	40.0	31.0	41.0
25	35.41361008073985	36.0	34.0	40.0	30.0	41.0
26	35.56772151820919	36.0	34.0	40.0	30.0	41.0
27	35.89678651212942	37.0	35.0	40.0	31.0	41.0
28	35.82010639906658	36.0	35.0	40.0	31.0	41.0
29	35.935942942363276	37.0	35.0	40.0	31.0	41.0
30	35.57623442711836	36.0	35.0	40.0	30.0	41.0
31	34.89767419558297	36.0	34.0	40.0	29.0	41.0
32	34.86003125334265	36.0	34.0	40.0	27.0	41.0
33	34.553356291794586	36.0	34.0	40.0	25.0	41.0
34	34.152869664217135	36.0	34.0	40.0	22.0	41.0
35	33.91876098077956	36.0	33.0	40.0	21.0	41.0
36	33.49939817029014	36.0	33.0	40.0	18.0	41.0
37	33.43237315163281	36.0	33.0	40.0	18.0	41.0
38	33.27835638400547	36.0	33.0	40.0	18.0	41.0
39	33.06346321953691	35.0	33.0	40.0	17.0	40.0
40	32.84017394661359	35.0	32.0	40.0	15.0	40.0
41	32.53277113381275	35.0	32.0	39.0	15.0	40.0
42	32.72226819105604	35.0	32.0	39.0	15.0	40.0
43	32.55820904330592	35.0	32.0	39.0	15.0	40.0
44	32.40144562078212	35.0	32.0	39.0	14.0	40.0
45	32.283517734612154	35.0	31.0	39.0	13.0	40.0
46	32.09108345330994	35.0	31.0	39.0	13.0	40.0
47	32.0465308420821	35.0	31.0	38.0	12.0	40.0
48	32.05483842808719	35.0	31.0	38.0	12.0	40.0
49	32.092519484160015	35.0	31.0	38.0	12.0	40.0
50	32.28325585569857	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	6.0
13	20.0
14	41.0
15	92.0
16	240.0
17	489.0
18	915.0
19	1564.0
20	2532.0
21	3526.0
22	5178.0
23	7348.0
24	10577.0
25	15914.0
26	22713.0
27	25450.0
28	24586.0
29	23795.0
30	25905.0
31	31651.0
32	42051.0
33	63522.0
34	99582.0
35	76380.0
36	79608.0
37	110705.0
38	116255.0
39	22703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.46137777816028	21.968075362112145	25.590364823145666	22.980182036581905
2	39.65166416057973	20.286394714226173	27.447000256961	12.614940868233104
3	29.450681315492783	18.529592931974186	38.90217408677413	13.117551665758903
4	25.359714662637256	24.284781638476773	35.645777417677195	14.709726281208773
5	20.221847094680907	28.28316856272738	36.813413118289354	14.681571224302365
6	18.52012594777421	36.72771846910259	34.46892062855857	10.283234954564623
7	75.03507087328626	5.111679676597984	17.19167446360928	2.661574986506474
8	76.35036693784863	3.9024876037833516	16.734431421535298	3.012714036832716
9	72.76078160405137	4.90402076343236	18.038293336349653	4.296904296166609
10	42.231847672535785	22.002500759202952	23.69635324391746	12.069298324343796
11	37.0518089931432	20.367540293083078	28.76192747798311	13.818723235790609
12	33.4902557684056	19.694646727804532	32.8279357179478	13.987161785842064
13	20.625116032030373	31.632636751816246	33.627219669688316	14.115027546465065
14	15.541591412338802	34.791289882744636	35.26439319704974	14.402725507866817
15	14.935212632153567	22.139464660485668	48.041994066536915	14.883328640823848
16	16.807954233893525	17.220321311902705	48.0733457674589	17.89837868674487
17	16.56562402794359	17.73067782377393	35.76048775869764	29.94321038958484
18	19.911649677323375	22.000533593654907	39.62080425104475	18.467012477976965
19	25.408279062104643	23.420335327957233	32.291022471177946	18.88036313876017
20	26.32989612136428	21.540462751105608	32.396634671538685	19.733006455991433
21	21.839471914408627	25.0699265878407	34.82362516644065	18.266976331310023
22	20.285042287911892	21.551773953006876	32.591138165101746	25.572045593979492
23	18.299065719312523	27.336347194883402	32.55818814217197	21.806398943632104
24	18.2234527935595	23.18796389759428	42.13607129991529	16.45251200893093
25	19.11162803850235	22.23954420774252	39.209174860116086	19.43965289363905
26	15.984326608495941	27.88198973877271	34.26740910773059	21.866274545000756
27	16.57976303032017	27.936824478424498	35.96667129770223	19.516741193553106
28	15.1389372142231	25.389836885091714	42.20307787639561	17.268148024289577
29	16.62832742978756	24.085852022430604	39.83264339099997	19.453177156781866
30	18.215584131367315	26.10453271826624	37.8589616070759	17.820921543290552
31	26.79820446964602	23.66205079467341	31.170229900178644	18.369514835501928
32	26.16858854642449	24.40047556227124	31.58284287388133	17.84809301742294
33	24.702681369589836	23.554225533071126	30.184679960607507	21.55841313673153
34	18.126569890318226	25.360083506177517	32.10451058765382	24.408836015850436
35	19.21699434316957	23.901307304454523	34.02962797211051	22.852070380265395
36	27.381346106794958	24.87136581533479	28.62201282837833	19.125275249491917
37	19.09970209736732	29.385273061020246	30.629874113699714	20.885150727912727
38	20.48175884271651	31.047527949119264	27.565276085537278	20.905437122626953
39	19.622230446067082	28.242472825452168	28.990856368636987	23.144440359843756
40	22.82071867934341	24.198226354362742	30.915481961706664	22.065573004587183
41	18.79429964603315	23.379393694988522	31.760010721052232	26.066295937926093
42	20.724949683593717	25.757573894729596	27.53933408987242	25.97814233180427
43	20.089309315881295	24.870382232560768	28.885981855356775	26.154326596201155
44	19.543420876298484	26.489605374296275	31.43997747595447	22.526996273450763
45	19.127734206426975	31.757428816270426	27.29061059589133	21.82422638141127
46	22.310239219625426	28.594595458552437	28.378944935347878	20.716220386474262
47	20.95055898238526	26.539768095771453	29.486582086744622	23.023090835098657
48	20.30754174386767	25.991051855713327	32.07008519056301	21.63132120985599
49	20.27852605203399	25.498030990234255	32.69306193005988	21.530381027671872
50	19.34289293824453	28.138581894945986	31.654644416385015	20.863880750424478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	488.0
1	774.5
2	1061.0
3	12224.0
4	23387.0
5	16393.0
6	9399.0
7	9415.5
8	9432.0
9	9871.0
10	10310.0
11	10328.0
12	10346.0
13	9840.0
14	9334.0
15	8627.0
16	7920.0
17	7489.0
18	7058.0
19	6445.5
20	5833.0
21	5586.0
22	5339.0
23	5206.0
24	5073.0
25	5368.0
26	5663.0
27	6340.5
28	7018.0
29	8048.0
30	9078.0
31	10121.5
32	11165.0
33	13551.5
34	15938.0
35	17979.0
36	20020.0
37	21468.0
38	22916.0
39	27980.0
40	33044.0
41	42763.5
42	52483.0
43	60339.0
44	68195.0
45	71312.0
46	74429.0
47	71694.5
48	68960.0
49	68401.0
50	67842.0
51	61461.5
52	55081.0
53	48376.5
54	41672.0
55	37996.5
56	34321.0
57	31329.5
58	28338.0
59	26304.5
60	24271.0
61	21867.0
62	19463.0
63	17516.0
64	15569.0
65	13116.5
66	10664.0
67	8836.0
68	7008.0
69	6346.0
70	5684.0
71	4913.5
72	4143.0
73	3389.0
74	2635.0
75	2013.0
76	1391.0
77	995.5
78	600.0
79	450.5
80	301.0
81	259.0
82	217.0
83	169.5
84	122.0
85	93.0
86	64.0
87	47.0
88	30.0
89	27.5
90	25.0
91	15.5
92	6.0
93	8.0
94	10.0
95	8.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	813353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.997708081493826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28437431957052	23.336430582559572
2	7.662798897572863	4.750584066283127
3	3.5194150342485053	3.2728139954776725
4	2.2121178318084347	2.7428233116905965
5	1.5183149802983258	2.3532142267523275
6	1.1458144754672395	2.1310573575650076
7	0.9421248601315617	2.044259797347345
8	0.707038233321284	1.7533251807158627
9	0.5737423654198195	1.6006228521542394
>10	5.8177272644989575	39.0989115160106
>50	0.5538809400762091	10.958993076333886
>100	0.05746315192629455	2.9157680194931923
>500	0.0035914469953934095	0.7008629559837276
>1k	0.0011971489984644698	0.43541080216426425
>5k	0.0	0.0
>10k+	3.9904966615482326E-4	1.9049222594686561
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15400	1.8933968399944427	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1282	0.15761913953719972	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1144	0.14065233668530144	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1094	0.13450494434765717	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	990	0.12171836828535702	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	827	0.10167786926463664	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03159759661549168	0.0
2	0.0	0.0	0.0	0.13389020511389274	0.0
3	0.0	0.0	0.0	0.18319229166179998	0.0
4	0.0	0.0	0.0	0.3058942427211801	0.0
5	0.0	0.0	0.0	0.6265422270527065	0.0
6	0.0	0.0	0.0	0.8870687143220717	0.0
7	0.0	0.0	0.0	1.073211754305941	0.0
8	0.0	0.0	0.0	1.5138568370682841	0.0
9	0.0	0.0	0.0	1.699139242124883	0.0
10	0.0	0.0	0.0	2.0904822383393187	0.0
11	0.0	0.0	0.0	2.3041655959958347	0.0
12	0.0	0.0	0.0	2.565306822498964	0.0
13	0.0	0.0	0.0	2.6608372994259564	0.0
14	0.0	0.0	0.0	2.699442923306363	0.0
15	1.229478467528859E-4	0.0	0.0	2.7470237399997295	0.0
16	1.229478467528859E-4	0.0	0.0	2.837021563822842	0.0
17	1.229478467528859E-4	0.0	0.0	2.937347006773197	0.0
18	1.229478467528859E-4	0.0	0.0	3.108367461606461	0.0
19	1.229478467528859E-4	0.0	0.0	3.177832995021842	0.0
20	2.458956935057718E-4	0.0	0.0	3.257257304024206	0.0
21	2.458956935057718E-4	0.0	0.0	3.34774691923433	0.0
22	2.458956935057718E-4	0.0	0.0	3.4448757181691096	0.0
23	2.458956935057718E-4	0.0	0.0	3.56204501612461	0.0
24	2.458956935057718E-4	0.0	0.0	3.6536411619555103	0.0
25	3.688435402586577E-4	0.0	0.0	3.723475538911149	0.0
26	6.147392337644295E-4	0.0	0.0	3.7979819340433982	0.0
27	6.147392337644295E-4	0.0	0.0	3.874701390417199	0.0
28	6.147392337644295E-4	0.0	0.0	3.9681417539493924	0.0
29	7.376870805173154E-4	0.0	0.0	4.051008602660837	0.0
30	7.376870805173154E-4	0.0	0.0	4.189324930257834	0.0
31	7.376870805173154E-4	0.0	0.0	4.283871824410803	0.0
32	7.376870805173154E-4	0.0	0.0	4.383213684587135	0.0
33	7.376870805173154E-4	1.229478467528859E-4	0.0	4.490424206955652	0.0
34	7.376870805173154E-4	1.229478467528859E-4	0.0	4.5956675637761215	0.0
35	7.376870805173154E-4	1.229478467528859E-4	0.0	4.739270648783492	0.0
36	7.376870805173154E-4	1.229478467528859E-4	0.0	4.834309334323473	0.0
37	7.376870805173154E-4	1.229478467528859E-4	0.0	4.9374625777491445	0.0
38	7.376870805173154E-4	1.229478467528859E-4	0.0	5.06422180775137	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGA	20	7.8551215E-4	44.000004	30
GTTTGCG	35	1.4453872E-7	44.0	1
TAAGTCG	25	4.4416884E-5	44.0	13
AACTCCG	30	2.5268018E-6	44.0	29
TGTCGGT	25	4.4416884E-5	44.0	44
GCGTAAG	30	2.5268018E-6	44.0	1
TACGTAG	45	4.802132E-10	44.0	1
CGTTTTT	9990	0.0	42.81081	1
CGTAAGG	105	0.0	41.904762	2
TCACGAC	145	0.0	40.965515	25
CGGTCTA	135	0.0	40.74074	31
GGTACCT	740	0.0	40.72973	8
ATGGGAC	380	0.0	40.526314	5
ATCCTGC	745	0.0	40.45638	20
GTTGATC	740	0.0	40.43243	16
TCGTTCA	110	0.0	40.0	16
TGGGCGA	275	0.0	40.0	6
ATGGGCC	105	0.0	39.809525	5
ATGGGCG	190	0.0	39.36842	5
GACGGGA	175	0.0	38.971428	4
>>END_MODULE