FastQCFastQC Report
Thu 26 May 2016
SRR1546083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546083_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences801245
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151581.8918058771037571No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12940.16149866769839438No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT11960.1492677021385469No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10530.13142047688285108No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT10260.12805072106534207No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC8200.10234073223545857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAG207.855053E-444.01
CACGACA207.855053E-444.017
ATGTCGA207.855053E-444.016
CCGAACG302.5267545E-644.037
CGATCAA254.441629E-544.010
CGGTCTA1150.043.99999631
CGTTTTT95900.042.9217951
CTCACGA1200.042.16666424
CGACGGT1200.040.33333228
GGTACCT8150.039.950928
TAACGGG2050.039.7073173
CAACGAC501.3460522E-939.612
AGGGTAC4700.039.3191536
TGGGTAC2800.039.285716
GTTTTTT108650.039.24162
TGATCCT8450.039.05325718
GGGTACC8400.039.023817
AGGGCGA5650.038.9380536
ATCCTGC8550.038.853820
GCGATAT850.038.8235329
Produced by FastQC (version 0.11.5)