##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546083_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	801245
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.71272457238423	31.0	28.0	31.0	16.0	33.0
2	28.608488040486993	31.0	28.0	33.0	16.0	33.0
3	28.146006527341825	31.0	27.0	33.0	16.0	34.0
4	32.13287820828835	35.0	32.0	35.0	25.0	37.0
5	33.64543803705483	35.0	33.0	35.0	28.0	37.0
6	33.474509045298255	35.0	33.0	37.0	28.0	37.0
7	34.3078209536409	35.0	35.0	37.0	31.0	37.0
8	34.71056293643018	35.0	35.0	37.0	32.0	37.0
9	36.52526880042933	39.0	37.0	39.0	32.0	39.0
10	36.233929696909186	38.0	35.0	39.0	31.0	39.0
11	36.34750669270947	38.0	35.0	39.0	32.0	39.0
12	35.230807056518294	37.0	34.0	39.0	30.0	39.0
13	35.32413681208619	37.0	34.0	39.0	30.0	39.0
14	36.06486655142934	38.0	34.0	40.0	29.0	41.0
15	36.55186990246429	38.0	35.0	40.0	31.0	41.0
16	36.18472127751187	38.0	34.0	40.0	31.0	41.0
17	35.69384645145992	37.0	34.0	40.0	30.0	41.0
18	35.41966564533944	37.0	34.0	39.0	29.0	40.0
19	34.85748179395815	37.0	33.0	39.0	27.0	40.0
20	35.284166515859695	35.0	34.0	39.0	30.0	40.0
21	35.44719655036849	36.0	34.0	39.0	30.0	40.0
22	35.786358729227636	36.0	34.0	40.0	31.0	41.0
23	36.14138871381412	37.0	35.0	40.0	31.0	41.0
24	35.75762469656597	36.0	34.0	40.0	31.0	41.0
25	35.4431927812342	36.0	34.0	40.0	30.0	41.0
26	35.572716210397566	36.0	34.0	40.0	30.0	41.0
27	35.90085055132949	37.0	35.0	40.0	31.0	41.0
28	35.82953278959619	36.0	35.0	40.0	31.0	41.0
29	35.93363827543386	37.0	35.0	40.0	31.0	41.0
30	35.57814151726376	36.0	35.0	40.0	30.0	41.0
31	34.87189436439541	36.0	34.0	40.0	28.0	41.0
32	34.84964274348046	36.0	34.0	40.0	27.0	41.0
33	34.55524714662806	36.0	34.0	40.0	24.0	41.0
34	34.16359790076693	36.0	34.0	40.0	22.0	41.0
35	33.886487903200646	36.0	33.0	40.0	20.0	41.0
36	33.51709651854302	36.0	33.0	40.0	18.0	41.0
37	33.467150497038986	36.0	33.0	40.0	18.0	41.0
38	33.292132868223824	36.0	33.0	40.0	18.0	41.0
39	33.053782550905154	35.0	33.0	40.0	16.0	41.0
40	32.870528989260464	35.0	32.0	40.0	15.0	40.0
41	32.56551366935207	35.0	32.0	39.0	15.0	40.0
42	32.78332594899188	35.0	32.0	40.0	15.0	40.0
43	32.60792891063283	35.0	32.0	39.0	15.0	40.0
44	32.44457188500396	35.0	32.0	39.0	13.0	40.0
45	32.3635080406118	35.0	32.0	39.0	14.0	40.0
46	32.17555678974596	35.0	31.0	39.0	13.0	40.0
47	32.15019500901722	35.0	31.0	39.0	13.0	40.0
48	32.1469338342205	35.0	31.0	39.0	13.0	40.0
49	32.193432720328985	35.0	31.0	39.0	13.0	40.0
50	32.30275883156837	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	12.0
13	23.0
14	45.0
15	83.0
16	225.0
17	428.0
18	937.0
19	1659.0
20	2612.0
21	3562.0
22	5193.0
23	7300.0
24	10634.0
25	15791.0
26	22133.0
27	24911.0
28	24015.0
29	23839.0
30	25649.0
31	31000.0
32	41305.0
33	62623.0
34	97208.0
35	74402.0
36	77545.0
37	106122.0
38	116602.0
39	25377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.516440040187458	22.00225898445544	25.48184388046103	22.999457094896066
2	39.609732354024054	20.227645726338388	27.555616571710278	12.607005347927288
3	29.566362348595	18.67506193486387	38.80585838289162	12.952717333649508
4	25.422436333456062	24.28058833440458	35.62905228737777	14.667923044761592
5	20.147021198260205	28.270753639648298	36.79960561376358	14.782619548327917
6	18.54239340027083	36.815206335140935	34.40670456601913	10.235695698569101
7	74.97494524146796	5.138752815930209	17.204600340719757	2.681701601882071
8	76.30475073167383	3.967076237605227	16.714612883699743	3.0135601470211983
9	72.75028237305693	4.908860585713484	18.02794401213112	4.312913029098466
10	42.215801658668695	22.155395665495572	23.723829789889482	11.904972885946247
11	37.064693071407625	20.452420919943336	28.643174060368555	13.83971194828049
12	33.50273636652959	20.057161043126634	32.839019276251335	13.601083314092444
13	20.586337512246565	31.757078047288907	33.48925734325955	14.167327097204973
14	15.464433475403903	34.813196962227536	35.27435428614219	14.448015276226373
15	14.87859518624141	22.091869528046974	47.99992511653739	15.029610169174223
16	16.860885247333837	17.3564889640497	47.99643055494886	17.786195233667605
17	16.70387958739212	17.740266709932666	35.671985472608256	29.883868230066955
18	19.929609545145368	21.972929628266012	39.65016942383416	18.447291402754463
19	25.437288220207304	23.429163364513975	32.23945235227677	18.894096063001953
20	26.359602868036617	21.692241449244612	32.44001522630406	19.508140456414704
21	21.824660372295615	25.163713970133976	34.736815830364	18.274809827206408
22	20.274822307783513	21.547466754862747	32.692871718388254	25.484839218965483
23	18.22688441113517	27.285786494767517	32.607379765240346	21.879949328856966
24	18.07911437824885	23.24507485226117	42.18597307939519	16.48983769009479
25	19.200993453937308	22.221542724135563	39.191882632652934	19.38558118927419
26	15.972018546137573	28.043607136393987	34.11203814064363	21.872336176824817
27	16.53114840030203	28.010658412845007	35.98986577139327	19.46832741545969
28	15.218191689183708	25.279658531410494	42.283571192331934	17.218578587073864
29	16.614019432258548	24.097623074090947	39.89004611573239	19.398311377918116
30	18.28367103694875	26.132581170553326	37.84722525569582	17.736522536802102
31	26.841602755711424	23.52638706013766	31.19807299889547	18.43393718525545
32	26.253642768441615	24.34686019881559	31.586468558306137	17.813028474436656
33	24.736878233249506	23.642082009872137	30.190640815231294	21.43039894164706
34	18.10831892866726	25.387365911799765	32.10254042146909	24.40177473806389
35	19.31506592864854	23.912161698356933	33.94542243633346	22.827349936661072
36	27.392994652072712	24.86667623510911	28.71281568059707	19.027513432221106
37	19.173161767000106	29.444177498767544	30.542468283733438	20.84019245049891
38	20.459534848891412	31.02584103488945	27.581825783624236	20.9327983325949
39	19.628827636989936	28.288850476446033	29.052037766226313	23.030284120337726
40	22.904355097379703	24.207701764129574	30.955200968492786	21.93274216999794
41	18.781022034458875	23.347415584496627	31.858295527585195	26.013266853459307
42	20.829084736878233	25.685277287221766	27.58881490680129	25.89682306909872
43	20.069891231770555	24.92808067445039	28.8771848810289	26.12484321275016
44	19.591760322997334	26.495017129592075	31.519323053497995	22.3938994939126
45	19.179402055551048	31.80662593838339	27.234241711336733	21.77973029472883
46	22.376925909054034	28.53584109729234	28.488040486992116	20.599192506661506
47	21.003063981678512	26.457949815599473	29.475628553064293	23.06335764965772
48	20.318753939182148	25.973953035588366	32.108156681164935	21.599136344064547
49	20.322872529625773	25.546742881390838	32.78497837740017	21.345406211583224
50	19.41528496277668	28.05771018851912	31.713271221661287	20.813733627042915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	827.0
2	1087.0
3	12209.0
4	23331.0
5	16318.0
6	9305.0
7	9309.5
8	9314.0
9	9764.5
10	10215.0
11	10114.5
12	10014.0
13	9499.5
14	8985.0
15	8516.0
16	8047.0
17	7462.0
18	6877.0
19	6212.0
20	5547.0
21	5411.0
22	5275.0
23	5145.0
24	5015.0
25	5222.0
26	5429.0
27	6174.5
28	6920.0
29	7893.0
30	8866.0
31	9935.0
32	11004.0
33	13534.5
34	16065.0
35	17952.5
36	19840.0
37	21223.5
38	22607.0
39	27705.5
40	32804.0
41	42403.5
42	52003.0
43	59758.0
44	67513.0
45	70242.5
46	72972.0
47	70451.5
48	67931.0
49	67412.0
50	66893.0
51	60423.5
52	53954.0
53	47131.0
54	40308.0
55	37056.0
56	33804.0
57	30787.5
58	27771.0
59	25954.5
60	24138.0
61	21598.0
62	19058.0
63	17137.5
64	15217.0
65	12886.5
66	10556.0
67	8714.5
68	6873.0
69	6293.5
70	5714.0
71	4888.5
72	4063.0
73	3293.0
74	2523.0
75	1943.0
76	1363.0
77	989.0
78	615.0
79	480.5
80	346.0
81	287.0
82	228.0
83	188.5
84	149.0
85	109.0
86	69.0
87	47.5
88	26.0
89	21.5
90	17.0
91	14.0
92	11.0
93	8.0
94	5.0
95	7.5
96	10.0
97	5.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	801245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.648292507769874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1169803708995	23.022071867077358
2	7.579204922612373	4.645793788891067
3	3.477149604382234	3.19706094505149
4	2.2256826154163223	2.7285348730695094
5	1.5720431781690765	2.4090219579685024
6	1.1686050780247002	2.1489450154419756
7	0.9270401899475521	1.9888539235579785
8	0.7196219454684859	1.7644147103782863
9	0.6269853659062546	1.7294427803147414
>10	5.9547093207560415	39.32174355884754
>50	0.5667701354415111	11.009551859542082
>100	0.06110618662525313	3.1644040196450973
>500	0.0020505431753440645	0.3906485419866922
>1k	0.0016404345402752517	0.5742835226310157
>5k	0.0	0.0
>10k+	4.101086350688129E-4	1.9052286355966155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15158	1.8918058771037571	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1294	0.16149866769839438	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1196	0.1492677021385469	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1053	0.13142047688285108	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	1026	0.12805072106534207	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	820	0.10234073223545857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03357275240407116	0.0
2	0.0	0.0	0.0	0.13840960005990677	0.0
3	0.0	0.0	0.0	0.19120244120088112	0.0
4	0.0	0.0	0.0	0.3103919525238847	0.0
5	0.0	0.0	0.0	0.6275234166827874	0.0
6	0.0	0.0	0.0	0.8864953915469052	0.0
7	1.2480577101885192E-4	0.0	0.0	1.0655916729589576	0.0
8	1.2480577101885192E-4	0.0	0.0	1.4820685308488664	0.0
9	1.2480577101885192E-4	0.0	0.0	1.6792616490586525	0.0
10	1.2480577101885192E-4	0.0	0.0	2.061292114147358	0.0
11	1.2480577101885192E-4	0.0	0.0	2.2684696940386524	0.0
12	1.2480577101885192E-4	0.0	0.0	2.5268176400476756	0.0
13	1.2480577101885192E-4	0.0	0.0	2.620297162540796	0.0
14	2.4961154203770384E-4	0.0	0.0	2.6594861746407155	0.0
15	2.4961154203770384E-4	0.0	0.0	2.7091588715062183	0.0
16	2.4961154203770384E-4	0.0	0.0	2.7967725227614526	0.0
17	2.4961154203770384E-4	0.0	0.0	2.896492333805515	0.0
18	2.4961154203770384E-4	0.0	0.0	3.0613607573214185	0.0
19	3.744173130565557E-4	0.0	0.0	3.125885340938165	0.0
20	3.744173130565557E-4	0.0	0.0	3.203264918969853	0.0
21	3.744173130565557E-4	0.0	0.0	3.2956211895238035	0.0
22	3.744173130565557E-4	0.0	0.0	3.3909727985822062	0.0
23	3.744173130565557E-4	0.0	0.0	3.4980561501163816	0.0
24	3.744173130565557E-4	0.0	0.0	3.582549657096144	0.0
25	3.744173130565557E-4	0.0	0.0	3.6505688023014184	0.0
26	3.744173130565557E-4	0.0	0.0	3.7188375590487306	0.0
27	3.744173130565557E-4	0.0	0.0	3.789976848529476	0.0
28	3.744173130565557E-4	0.0	0.0	3.878214528639804	0.0
29	4.992230840754077E-4	0.0	0.0	3.9654537625819817	0.0
30	4.992230840754077E-4	0.0	0.0	4.098621520259097	0.0
31	4.992230840754077E-4	0.0	0.0	4.193349100462405	0.0
32	4.992230840754077E-4	0.0	0.0	4.28096275171764	0.0
33	6.240288550942596E-4	0.0	0.0	4.389294160962003	0.0
34	6.240288550942596E-4	0.0	0.0	4.492383727823575	0.0
35	6.240288550942596E-4	0.0	0.0	4.627922795150048	0.0
36	6.240288550942596E-4	0.0	0.0	4.720154259932979	0.0
37	6.240288550942596E-4	0.0	0.0	4.815006645907307	0.0
38	6.240288550942596E-4	0.0	0.0	4.934695380314386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAG	20	7.855053E-4	44.0	1
CACGACA	20	7.855053E-4	44.0	17
ATGTCGA	20	7.855053E-4	44.0	16
CCGAACG	30	2.5267545E-6	44.0	37
CGATCAA	25	4.441629E-5	44.0	10
CGGTCTA	115	0.0	43.999996	31
CGTTTTT	9590	0.0	42.921795	1
CTCACGA	120	0.0	42.166664	24
CGACGGT	120	0.0	40.333332	28
GGTACCT	815	0.0	39.95092	8
TAACGGG	205	0.0	39.707317	3
CAACGAC	50	1.3460522E-9	39.6	12
AGGGTAC	470	0.0	39.319153	6
TGGGTAC	280	0.0	39.28571	6
GTTTTTT	10865	0.0	39.2416	2
TGATCCT	845	0.0	39.053257	18
GGGTACC	840	0.0	39.02381	7
AGGGCGA	565	0.0	38.938053	6
ATCCTGC	855	0.0	38.8538	20
GCGATAT	85	0.0	38.823532	9
>>END_MODULE