FastQCFastQC Report
Thu 26 May 2016
SRR1546082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546082_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences683853
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75671.1065243553804691No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7750.11332844924274663No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7130.10426217330332689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG850.044.02
GTCGAAT207.854243E-444.022
ACGTTAG207.854243E-444.01
ACCATCG207.854243E-444.013
ATTAACG302.526207E-644.01
ATTCGAC351.4449324E-744.027
AAGCGAT207.854243E-444.044
TCGGACG254.440941E-544.01
GTTCGCC207.854243E-444.020
CGAATTT254.440941E-544.024
TCGTAAA207.854243E-444.015
CTCGATG302.526207E-644.01
TTACCGC254.440941E-544.033
GCGTATG454.783942E-1044.01
TCGAATT207.854243E-444.023
AGTCGAA207.854243E-444.021
CGTTTTT85600.043.1518671
TGTACGG1450.042.4827582
GCGGAAT2850.041.684218
CGGTCTA1700.041.41176631
Produced by FastQC (version 0.11.5)