##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546082_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	683853
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.517282222933876	28.0	16.0	30.0	16.0	33.0
2	25.87860256517117	30.0	25.0	31.0	10.0	33.0
3	26.33400891712108	30.0	25.0	31.0	16.0	33.0
4	28.218716595525645	32.0	19.0	35.0	10.0	37.0
5	31.236828675168493	33.0	30.0	35.0	19.0	37.0
6	29.742481205756206	33.0	28.0	35.0	17.0	37.0
7	29.599623018397228	32.0	28.0	35.0	17.0	35.0
8	32.29712964628363	33.0	32.0	35.0	28.0	35.0
9	26.854019796652203	30.0	23.0	34.0	10.0	35.0
10	31.133211377298924	34.0	28.0	37.0	17.0	37.0
11	33.43233852889437	35.0	32.0	37.0	27.0	39.0
12	32.97092650028588	35.0	31.0	37.0	23.0	39.0
13	33.07130187335582	35.0	31.0	38.0	23.0	39.0
14	32.83887911583337	35.0	31.0	38.0	21.0	40.0
15	31.740311148741032	34.0	27.0	38.0	17.0	40.0
16	30.390696538583583	33.0	26.0	38.0	17.0	40.0
17	30.37735302762436	32.0	25.0	37.0	17.0	40.0
18	31.466940994628963	34.0	27.0	38.0	18.0	39.0
19	31.15518978493916	34.0	27.0	37.0	18.0	39.0
20	31.606257485161283	34.0	29.0	37.0	18.0	39.0
21	32.60504816093517	34.0	31.0	37.0	23.0	39.0
22	33.004185109957845	34.0	31.0	37.0	25.0	39.0
23	34.290873915885435	35.0	33.0	38.0	28.0	40.0
24	33.96222726229175	35.0	33.0	38.0	27.0	40.0
25	32.7846145297308	35.0	31.0	38.0	23.0	39.0
26	26.484340932919793	33.0	21.0	36.0	0.0	39.0
27	29.75046830239832	34.0	24.0	37.0	15.0	39.0
28	31.210400480805085	34.0	27.0	37.0	19.0	39.0
29	32.48187402848273	34.0	30.0	37.0	23.0	39.0
30	32.232064493392585	35.0	30.0	37.0	22.0	39.0
31	31.12447850634566	34.0	29.0	36.0	18.0	39.0
32	31.40932188642881	34.0	30.0	37.0	18.0	39.0
33	31.100980766334285	34.0	29.0	37.0	18.0	39.0
34	31.11839971455854	34.0	30.0	37.0	15.0	39.0
35	30.2825110074826	34.0	28.0	37.0	12.0	39.0
36	30.059960254616122	34.0	27.0	37.0	10.0	39.0
37	30.221344353245506	34.0	27.0	37.0	10.0	39.0
38	29.89961146620692	33.0	27.0	37.0	10.0	39.0
39	29.832557581819486	34.0	27.0	37.0	10.0	39.0
40	29.688409643592994	33.0	27.0	36.0	10.0	39.0
41	29.05280959504455	33.0	25.0	36.0	10.0	38.0
42	29.28411222879771	33.0	26.0	36.0	10.0	38.0
43	28.91677597378384	33.0	25.0	36.0	10.0	38.0
44	28.685970522904775	33.0	24.0	35.0	9.0	38.0
45	28.0424243221862	32.0	23.0	35.0	9.0	38.0
46	27.58727972239648	31.0	23.0	35.0	8.0	38.0
47	27.672899000223733	31.0	23.0	35.0	8.0	38.0
48	27.72488531892088	31.0	23.0	34.0	8.0	37.0
49	27.498229882737956	31.0	23.0	34.0	8.0	37.0
50	29.415086283163195	33.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	15.0
12	47.0
13	109.0
14	268.0
15	561.0
16	1073.0
17	2008.0
18	3358.0
19	5118.0
20	7484.0
21	10234.0
22	13720.0
23	18316.0
24	23322.0
25	29300.0
26	33966.0
27	37655.0
28	41879.0
29	49055.0
30	58485.0
31	67887.0
32	72998.0
33	72242.0
34	62545.0
35	45797.0
36	21833.0
37	4458.0
38	110.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.638980892092306	22.88576638546588	25.294763640723954	24.180489081717855
2	39.505858715250206	17.647798576594678	29.143690237521806	13.702652470633309
3	30.95811526746245	14.373410659893281	40.551112592911046	14.117361479733217
4	26.720216186812078	24.014517739923637	34.64443381837909	14.620832254885189
5	19.46295475782076	28.591671017016818	37.44152617594717	14.503848049215257
6	18.588790280952196	35.28799317982081	35.513041545478345	10.610174993748657
7	77.57368908230278	3.6196375536847833	17.02207930651763	1.7845940574948125
8	79.54911362529667	2.458715542667796	16.070997714421082	1.9211731176144582
9	67.09687608301785	2.9731535871013217	16.045846110202046	13.884124219678789
10	43.50554870710518	21.941703845709533	23.54102416747459	11.011723279710699
11	38.38968316290197	19.852658392958723	28.689791519522473	13.067866924616839
12	34.30620323373591	19.8424222749626	33.07172740340395	12.779647087897544
13	20.071711318075668	32.7631815609495	33.56978765904368	13.595319461931146
14	14.800110550074358	35.36666505813384	35.73019347725315	14.10303091453865
15	13.373049471158275	20.48291080100548	48.23975328031025	17.904286447526
16	15.032324198329173	15.23046619668262	48.1879877693013	21.54922183568691
17	15.680416697740595	16.725963035915616	36.38091812129215	31.21270214505164
18	19.366296557885978	21.475814246629028	40.755250031805076	18.40263916367991
19	26.15138048674203	22.573272326070075	32.88133560867613	18.39401157851176
20	26.903150238428434	21.060959007272032	32.89347272001439	19.142418034285146
21	21.724259453420544	24.798750608683445	35.289601712648775	18.18738822524724
22	19.949316592893503	21.107313998768742	32.68611821546443	26.25725119287332
23	17.704389686087506	27.457362912789733	33.00577755745752	21.83246984366523
24	17.202673674020584	22.726082944726425	44.1479382264902	15.923305154762794
25	18.60940874720152	21.04063300153688	40.96582160201096	19.384136649250642
26	12.680210513078102	39.993829083150914	28.409029425914632	18.916930977856353
27	15.547200933533961	29.05010287298586	36.61313176954696	18.789564423933214
28	14.420643032932517	25.178218125825286	43.77695206425942	16.62418677698277
29	15.769909615078095	23.789030683494843	41.12199551658032	19.31906418484674
30	17.708630363543044	26.076510595113277	38.612684304960275	17.602174736383404
31	27.616753893015016	23.34873137940464	31.54479105889716	17.489723668683183
32	26.76584002702335	23.611214690876547	32.019746933917084	17.603198348183017
33	25.093258346457496	23.141230644597595	30.43592701940329	21.32958398954161
34	17.576730671650193	24.78602857631684	32.27506496279171	25.362175789241252
35	18.97205978477831	23.273569027261708	34.34188341646524	23.41248777149475
36	27.790767898949042	24.352017173281393	28.49501281708203	19.362202110687534
37	18.6154041877421	29.910960396459473	30.14141928162924	21.332216134169187
38	19.767698613590934	31.81926525145024	27.352808278972233	21.060227855986593
39	19.202079979176812	28.509050921762423	28.69827287443354	23.590596224627223
40	22.756206377686432	23.60668155290684	30.990578384535855	22.64653368487087
41	18.141179464007614	22.501765730354332	32.01638363800407	27.340671167633978
42	20.031205536862455	24.954924523252807	26.806345808236564	28.207524131648178
43	19.520277018599028	24.12579896556716	28.869216044968727	27.484707970865085
44	18.753006859661358	26.09888382444765	31.742933057250607	23.40517625864038
45	18.528835875546353	32.857353846513796	26.572816087667967	22.04099419027189
46	21.723235841620934	28.43564333270454	27.891666776339356	21.949454049335166
47	20.40321531089284	25.964790678698492	28.80370488979357	24.828289120615104
48	19.512234354459217	25.49948600064634	32.80368734216272	22.18459230273173
49	19.880588372062416	25.40867701099505	32.44352221895641	22.267212397986118
50	18.911227997829943	28.76173680600948	31.50896464591074	20.818070550249836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	498.5
2	677.0
3	6865.5
4	13054.0
5	10362.5
6	7671.0
7	7735.5
8	7800.0
9	8307.0
10	8814.0
11	9031.0
12	9248.0
13	9185.5
14	9123.0
15	8815.0
16	8507.0
17	8098.5
18	7690.0
19	7234.0
20	6778.0
21	6525.0
22	6272.0
23	5947.5
24	5623.0
25	5686.5
26	5750.0
27	6327.0
28	6904.0
29	7468.0
30	8032.0
31	9086.5
32	10141.0
33	11758.5
34	13376.0
35	14864.5
36	16353.0
37	17480.0
38	18607.0
39	22314.5
40	26022.0
41	34085.0
42	42148.0
43	49050.5
44	55953.0
45	58076.5
46	60200.0
47	58799.0
48	57398.0
49	56153.5
50	54909.0
51	50445.5
52	45982.0
53	40893.5
54	35805.0
55	32639.0
56	29473.0
57	26808.0
58	24143.0
59	22625.0
60	21107.0
61	18907.5
62	16708.0
63	15117.0
64	13526.0
65	11391.0
66	9256.0
67	7867.5
68	6479.0
69	5780.5
70	5082.0
71	4458.5
72	3835.0
73	3112.5
74	2390.0
75	1828.0
76	1266.0
77	968.5
78	671.0
79	494.5
80	318.0
81	261.0
82	204.0
83	164.0
84	124.0
85	87.0
86	50.0
87	38.0
88	26.0
89	20.5
90	15.0
91	10.5
92	6.0
93	6.5
94	7.0
95	6.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	683853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.21979727147737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.93758563611254	38.690761826515505
2	7.0302726241046924	6.6393609614688165
3	2.821580804922227	3.9970342078055854
4	1.5708676176085963	2.967042017752262
5	1.0300451911615942	2.431926255355531
6	0.7501472994054469	2.125308204100286
7	0.5913044681346056	1.9544893977724378
8	0.46632526173399463	1.761582745731828
9	0.3745582563358925	1.5917908437485009
>10	3.2735053658385067	30.60204864974938
>50	0.12744047979156337	3.8604350922823056
>100	0.025014841268308136	1.8426653442096037
>500	0.0010141151865530292	0.327702005514753
>1k	0.0	0.0
>5k	3.380383955176764E-4	1.2078524479932469
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7567	1.1065243553804691	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	775	0.11332844924274663	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	713	0.10426217330332689	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020910926763500343	0.0
2	0.0	0.0	0.0	0.09402605530720784	0.0
3	0.0	0.0	0.0	0.13701775089090784	0.0
4	0.0	0.0	0.0	0.19653346552548573	0.0
5	0.0	0.0	0.0	0.3835619643402895	0.0
6	0.0	0.0	0.0	0.5262826952576065	0.0
7	0.0	0.0	0.0	0.6176766059372409	0.0
8	0.0	0.0	0.0	0.8639283588724478	0.0
9	0.0	0.0	0.0	1.0065028595326773	0.0
10	0.0	0.0	0.0	1.2431034155001148	0.0
11	0.0	0.0	0.0	1.3916733567009285	0.0
12	0.0	0.0	0.0	1.549748264612424	0.0
13	0.0	0.0	0.0	1.6158443408159355	0.0
14	0.0	0.0	0.0	1.6398261029782717	0.0
15	0.0	0.0	0.0	1.6695108451670169	0.0
16	0.0	0.0	0.0	1.723762270546448	0.0
17	0.0	0.0	0.0	1.7983397016610294	0.0
18	0.0	0.0	0.0	1.9053802498490173	0.0
19	0.0	0.0	0.0	1.9438388074630073	0.0
20	0.0	0.0	0.0	1.9925334830731165	0.0
21	0.0	0.0	0.0	2.043860303310799	0.0
22	0.0	0.0	0.0	2.1005976430607163	0.0
23	0.0	0.0	0.0	2.160405818209469	0.0
24	0.0	0.0	0.0	2.211440177932977	0.0
25	0.0	0.0	0.0	2.2509223473465787	0.0
26	0.0	0.0	0.0	2.2924517403594047	0.0
27	0.0	0.0	0.0	2.3503589221660213	0.0
28	0.0	0.0	0.0	2.4142615445132214	0.0
29	0.0	0.0	0.0	2.4717300355485756	0.0
30	0.0	0.0	0.0	2.581110267849962	0.0
31	0.0	0.0	0.0	2.655249008193281	0.0
32	0.0	0.0	0.0	2.7184204792550446	0.0
33	0.0	0.0	0.0	2.800748113995259	0.0
34	0.0	0.0	0.0	2.8782501502515894	0.0
35	0.0	0.0	0.0	2.9847057774112273	0.0
36	0.0	0.0	0.0	3.054750070556099	0.0
37	0.0	0.0	0.0	3.1258179755005826	0.0
38	0.0	0.0	0.0	3.215310892838081	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	85	0.0	44.0	2
GTCGAAT	20	7.854243E-4	44.0	22
ACGTTAG	20	7.854243E-4	44.0	1
ACCATCG	20	7.854243E-4	44.0	13
ATTAACG	30	2.526207E-6	44.0	1
ATTCGAC	35	1.4449324E-7	44.0	27
AAGCGAT	20	7.854243E-4	44.0	44
TCGGACG	25	4.440941E-5	44.0	1
GTTCGCC	20	7.854243E-4	44.0	20
CGAATTT	25	4.440941E-5	44.0	24
TCGTAAA	20	7.854243E-4	44.0	15
CTCGATG	30	2.526207E-6	44.0	1
TTACCGC	25	4.440941E-5	44.0	33
GCGTATG	45	4.783942E-10	44.0	1
TCGAATT	20	7.854243E-4	44.0	23
AGTCGAA	20	7.854243E-4	44.0	21
CGTTTTT	8560	0.0	43.151867	1
TGTACGG	145	0.0	42.482758	2
GCGGAAT	285	0.0	41.68421	8
CGGTCTA	170	0.0	41.411766	31
>>END_MODULE