FastQCFastQC Report
Thu 26 May 2016
SRR1546081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546081_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1727873
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360452.0860908180172966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG302.5284608E-644.01
TTACCCG207.8575837E-444.032
ACGCGGT254.443774E-544.034
CGTTTTT217050.043.1181761
CGACGGT3600.042.7777828
CGGTCTA3450.042.7246431
TCACGAC3550.040.9014125
GACACGA1350.040.7407425
CGTAAGG1800.040.3333322
ACGGTCT3700.039.83783730
ACGTAAG452.3521352E-839.111111
GTTTTTT247150.038.9350622
AACGGGC1700.038.8235324
GGCGATA4600.038.7391328
CTCACGA3700.038.64864724
CAATTCG404.126141E-738.512
TAGGGTA7500.038.426675
GGGCGAT16250.038.3138477
TCGTAAG700.037.7142871
ACGACGG3800.037.6315827
Produced by FastQC (version 0.11.5)