##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546081_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1727873
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.803503498231642	31.0	28.0	31.0	16.0	33.0
2	28.725093221550427	31.0	28.0	33.0	16.0	33.0
3	28.165413777517212	31.0	27.0	33.0	16.0	34.0
4	32.165192696453964	35.0	32.0	35.0	25.0	37.0
5	33.61073990970401	35.0	33.0	35.0	28.0	37.0
6	33.43200802373786	35.0	33.0	37.0	28.0	37.0
7	34.23186021194845	35.0	33.0	37.0	30.0	37.0
8	34.56228090837695	35.0	35.0	37.0	32.0	37.0
9	36.25994271569728	39.0	35.0	39.0	31.0	39.0
10	36.07383181518549	37.0	35.0	39.0	31.0	39.0
11	36.291818901041914	38.0	35.0	39.0	32.0	39.0
12	35.088849701338006	37.0	34.0	39.0	29.0	39.0
13	35.41477469698294	37.0	35.0	39.0	30.0	39.0
14	36.04276703206775	38.0	34.0	40.0	29.0	41.0
15	36.59993298118554	38.0	35.0	40.0	31.0	41.0
16	36.18626079578765	38.0	34.0	40.0	30.0	41.0
17	35.7496314833324	37.0	34.0	40.0	29.0	41.0
18	35.39217928632486	37.0	33.0	39.0	29.0	40.0
19	34.918509635835505	37.0	33.0	39.0	27.0	40.0
20	35.2375840122509	36.0	34.0	39.0	29.0	40.0
21	35.48753178040284	36.0	34.0	39.0	30.0	40.0
22	35.82395870529837	37.0	34.0	40.0	31.0	41.0
23	36.164546815651384	37.0	35.0	40.0	31.0	41.0
24	35.84263774015798	36.0	35.0	40.0	31.0	41.0
25	35.49873688633366	36.0	34.0	40.0	30.0	41.0
26	35.605403869381604	36.0	34.0	40.0	30.0	41.0
27	35.89320395654079	37.0	35.0	40.0	31.0	41.0
28	35.78998803731524	36.0	35.0	40.0	31.0	41.0
29	35.936388843393004	37.0	35.0	40.0	31.0	41.0
30	35.55087324126252	36.0	35.0	40.0	30.0	41.0
31	34.8429462119033	36.0	34.0	40.0	28.0	41.0
32	34.8051465588038	36.0	34.0	40.0	27.0	41.0
33	34.44897686346161	36.0	34.0	40.0	24.0	41.0
34	34.090081852080566	36.0	34.0	40.0	21.0	41.0
35	33.8268044005549	36.0	33.0	40.0	19.0	41.0
36	33.431416545081724	36.0	33.0	40.0	18.0	41.0
37	33.35161611993474	36.0	33.0	40.0	17.0	41.0
38	33.186263689518846	36.0	33.0	40.0	16.0	41.0
39	33.02626061058886	36.0	33.0	40.0	15.0	41.0
40	32.830916971328335	36.0	32.0	40.0	15.0	40.0
41	32.52483776296059	35.0	32.0	39.0	15.0	40.0
42	32.70595871340081	35.0	32.0	40.0	15.0	40.0
43	32.470437931491496	35.0	32.0	39.0	14.0	40.0
44	32.215375782826634	35.0	31.0	39.0	10.0	40.0
45	32.17879786303739	35.0	31.0	39.0	11.0	40.0
46	32.02324707892305	35.0	31.0	39.0	10.0	40.0
47	32.000820083420486	35.0	31.0	39.0	11.0	40.0
48	32.008773792981316	35.0	31.0	39.0	10.0	40.0
49	31.993832301332333	35.0	31.0	39.0	10.0	40.0
50	32.12230644266101	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	15.0
13	29.0
14	75.0
15	210.0
16	516.0
17	1151.0
18	2114.0
19	3574.0
20	5680.0
21	8058.0
22	11463.0
23	16265.0
24	23470.0
25	35354.0
26	49371.0
27	55634.0
28	53330.0
29	52344.0
30	57263.0
31	68729.0
32	89000.0
33	130556.0
34	200106.0
35	160130.0
36	168455.0
37	231095.0
38	250048.0
39	53826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.707781185307024	21.93378795779551	26.166680074287868	23.191750782609603
2	39.186849959458826	19.57927463418897	28.342418684706573	12.89145672164563
3	28.25097677896466	18.314366854508403	40.14149188047964	13.293164486047296
4	24.998307167251298	24.53478930453801	35.8525192534405	14.614384274770195
5	20.13070405058705	27.942099911278202	37.43457997202341	14.492616066111342
6	18.57271917554126	35.23864311786804	35.47824406076141	10.710393645829294
7	72.94280308795842	5.7119938791797775	18.07349266988951	3.2717103629722786
8	73.71658680933147	4.177043104441125	18.187968675938567	3.9184014102888347
9	69.70298164274806	5.93110720521705	19.733279008353044	4.632632143681857
10	40.50390277526184	21.36163942604578	25.251450772134294	12.883007026558085
11	35.3207672091641	21.160409358789682	29.578447027067384	13.940376404978839
12	32.24912942097017	19.933177959259737	33.73291902819246	14.08477359157762
13	20.735841117952535	29.923669158555054	34.4488860003021	14.891603723190302
14	16.268904022459985	32.41418784829672	36.09507180215212	15.22183632709117
15	15.678582858809648	21.79523610820934	47.657669284721734	14.868511748259275
16	17.115204647563797	18.74206032503546	46.51811794038104	17.624617087019708
17	17.49949215017539	19.174383765473504	36.04362126151633	27.282502822834783
18	20.7004797227574	21.65078104698667	39.16306348904115	18.485675741214777
19	24.38518340178937	23.332096745536273	34.26808567527822	18.01463417739614
20	25.517500418144156	22.32704602710963	33.161985863544366	18.993467691201843
21	21.522415131204667	24.479171790982324	35.88510266668904	18.113310411123965
22	19.91841993016848	21.80825789858398	34.57858303243352	23.694739138814022
23	18.708724541676386	26.22148734310913	33.9845578928544	21.085230222360092
24	19.155748136581796	22.28861727684847	41.19185842941003	17.3637761571597
25	18.701142965947152	21.29080088640774	39.19292679496699	20.81512935267812
26	16.776753847070935	26.506461991130138	35.331589763831026	21.385194397967904
27	17.275864603474908	26.978661047426517	36.810286404151235	18.935187944947344
28	15.445811121534975	25.25243464074038	40.7640492096352	18.537705028089448
29	16.66615544082233	23.998060042607296	38.90534778887106	20.430436727699316
30	18.751030891737994	24.76183145404784	37.76336571032709	18.72377194388708
31	24.37557621422408	22.990347091481837	33.537302799453435	19.096773894840652
32	25.160356114135702	23.20228396415709	33.608488586834795	18.028871334872413
33	23.747231422679793	24.10981594133365	32.15942375394488	19.98352888204168
34	18.43908666898551	25.28142982730791	33.548761974983115	22.730721528723468
35	18.847392140510326	24.423149155059427	34.33811397018184	22.39134473424841
36	25.410895360943776	24.361917802986678	30.79190426611215	19.435282569957398
37	19.29933507844616	28.784465061957675	31.410815493962808	20.505384365633354
38	19.875766332363547	29.500316284819544	29.15729338903959	21.46662399377732
39	19.78345630726332	26.276468235802053	30.73084653791106	23.209228919023563
40	22.067073216607934	23.037804283069416	32.201324981639274	22.693797518683375
41	17.848476132215733	23.150196802658527	31.952753472043373	27.048573593082363
42	20.91988242191411	24.828908143133205	29.05502892863075	25.196180506321937
43	20.503127255301752	24.38026405875895	30.341813316140716	24.774795369798593
44	19.598720507815102	26.551314824642784	30.86337942661295	22.986585240929166
45	19.205115190757656	29.833616243786437	28.23286202168794	22.728406543767974
46	21.297514342778666	27.158651127715984	30.181732106468473	21.362102423036877
47	20.4523712101526	25.063358244500613	31.00615612374289	23.4781144216039
48	20.30045032244847	24.763220445021133	32.63573190853726	22.30059732399314
49	20.540340638461277	23.578121771681136	33.194222029049584	22.687315560808
50	19.748442159811514	26.45043935520724	31.587043723699598	22.214074761281648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	880.0
1	1609.5
2	2339.0
3	28845.0
4	55351.0
5	38235.5
6	21120.0
7	20928.5
8	20737.0
9	21477.5
10	22218.0
11	21969.5
12	21721.0
13	20559.5
14	19398.0
15	18238.0
16	17078.0
17	16013.0
18	14948.0
19	13855.5
20	12763.0
21	12106.0
22	11449.0
23	11188.5
24	10928.0
25	11222.0
26	11516.0
27	14281.0
28	17046.0
29	17385.5
30	17725.0
31	19950.5
32	22176.0
33	26662.0
34	31148.0
35	35401.5
36	39655.0
37	45104.0
38	50553.0
39	59277.0
40	68001.0
41	90711.0
42	113421.0
43	128521.0
44	143621.0
45	145690.5
46	147760.0
47	147782.0
48	147804.0
49	148766.0
50	149728.0
51	136210.0
52	122692.0
53	106689.5
54	90687.0
55	82546.0
56	74405.0
57	72237.0
58	70069.0
59	63987.0
60	57905.0
61	49526.5
62	41148.0
63	35264.0
64	29380.0
65	23644.5
66	17909.0
67	14813.5
68	11718.0
69	9986.5
70	8255.0
71	6787.5
72	5320.0
73	4380.0
74	3440.0
75	2670.0
76	1900.0
77	1400.5
78	901.0
79	696.0
80	491.0
81	365.5
82	240.0
83	186.5
84	133.0
85	110.5
86	88.0
87	62.0
88	36.0
89	31.5
90	27.0
91	22.5
92	18.0
93	13.5
94	9.0
95	12.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1727873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.05375690399587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43838956287718	21.22053123175006
2	7.1838685658236425	3.887012676201005
3	2.691862161477178	2.1847495360700537
4	1.5929124306344071	1.723770626709457
5	1.1061318236174942	1.496251072996066
6	0.8561594108356716	1.3897397143089953
7	0.707410802277243	1.3396683913248453
8	0.5591272370468051	1.2101193879573713
9	0.48938325247869574	1.1915689990900922
>10	5.092533809932791	30.229300157439642
>50	0.9272650968154421	17.533192301602263
>100	0.34933192740178765	13.25327404638458
>500	0.004542395939074554	0.9154050854315032
>1k	8.652182741094387E-4	0.31611700258908015
>5k	0.0	0.0
>10k+	2.1630456852735967E-4	2.1092997701450193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36045	2.0860908180172966	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08438120162766592	0.0
2	0.0	0.0	0.0	0.30552013950099344	0.0
3	0.0	0.0	0.0	0.4055274895782271	0.0
4	0.0	0.0	0.0	0.57810961801012	0.0
5	0.0	0.0	0.0	0.9799331316595606	0.0
6	0.0	0.0	0.0	1.3632367656650692	0.0
7	0.0	0.0	0.0	1.6303860295287906	0.0
8	0.0	0.0	0.0	2.270942366713294	0.0
9	0.0	0.0	0.0	2.513842163168242	0.0
10	5.787462388728801E-5	0.0	0.0	2.9210480168392006	0.0
11	1.1574924777457602E-4	0.0	0.0	3.297638194473784	0.0
12	1.7362387166186403E-4	0.0	0.0	3.599917355037089	0.0
13	2.3149849554915205E-4	0.0	0.0	3.731929372123993	0.0
14	2.3149849554915205E-4	0.0	0.0	3.7779396981143867	0.0
15	2.3149849554915205E-4	0.0	0.0	3.849472733239075	0.0
16	2.3149849554915205E-4	0.0	0.0	4.025353715232543	0.0
17	2.3149849554915205E-4	0.0	0.0	4.228898767444135	0.0
18	2.8937311943644006E-4	0.0	0.0	4.542637103537124	0.0
19	2.8937311943644006E-4	0.0	0.0	4.666430924032032	0.0
20	2.8937311943644006E-4	0.0	0.0	4.809612743529183	0.0
21	2.8937311943644006E-4	0.0	0.0	4.99278592813245	0.0
22	2.8937311943644006E-4	0.0	0.0	5.168146038510932	0.0
23	2.8937311943644006E-4	0.0	0.0	5.3696076042625815	0.0
24	2.8937311943644006E-4	0.0	0.0	5.521702115838375	0.0
25	2.8937311943644006E-4	0.0	0.0	5.642023458900047	0.0
26	2.8937311943644006E-4	0.0	0.0	5.7644861630455475	0.0
27	2.8937311943644006E-4	0.0	0.0	5.902054144025631	0.0
28	5.208716149855921E-4	0.0	0.0	6.038696131023518	0.0
29	5.208716149855921E-4	0.0	0.0	6.184366559347822	0.0
30	5.208716149855921E-4	0.0	0.0	6.362851899416219	0.0
31	5.208716149855921E-4	0.0	0.0	6.5273894551277785	0.0
32	5.208716149855921E-4	0.0	0.0	6.677921351858615	0.0
33	5.787462388728801E-4	0.0	0.0	6.836439946685896	0.0
34	5.787462388728801E-4	0.0	0.0	6.99513216538484	0.0
35	5.787462388728801E-4	0.0	0.0	7.18571330184568	0.0
36	5.787462388728801E-4	0.0	0.0	7.336708195567614	0.0
37	5.787462388728801E-4	0.0	0.0	7.495053166523234	0.0
38	6.366208627601682E-4	0.0	0.0	7.6748117483171505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTG	30	2.5284608E-6	44.0	1
TTACCCG	20	7.8575837E-4	44.0	32
ACGCGGT	25	4.443774E-5	44.0	34
CGTTTTT	21705	0.0	43.118176	1
CGACGGT	360	0.0	42.77778	28
CGGTCTA	345	0.0	42.72464	31
TCACGAC	355	0.0	40.90141	25
GACACGA	135	0.0	40.74074	25
CGTAAGG	180	0.0	40.333332	2
ACGGTCT	370	0.0	39.837837	30
ACGTAAG	45	2.3521352E-8	39.11111	1
GTTTTTT	24715	0.0	38.935062	2
AACGGGC	170	0.0	38.823532	4
GGCGATA	460	0.0	38.739132	8
CTCACGA	370	0.0	38.648647	24
CAATTCG	40	4.126141E-7	38.5	12
TAGGGTA	750	0.0	38.42667	5
GGGCGAT	1625	0.0	38.313847	7
TCGTAAG	70	0.0	37.714287	1
ACGACGG	380	0.0	37.63158	27
>>END_MODULE