FastQCFastQC Report
Thu 26 May 2016
SRR1546080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546080_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1735487
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368012.12049989426599No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18010.10377490583334822No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.857593E-444.019
GTCGATG600.044.01
CGCATCG302.5284662E-644.021
CCCGTAT302.5284662E-644.034
TCTACCG207.857593E-444.01
TCTATCG207.857593E-444.017
CGACGGT3500.043.3714328
CGTTTTT222350.043.218351
CGGGCTA1450.040.965526
GACGGTC3750.040.4829
TATAGCG1550.039.7419321
GTTTTTT250250.039.3318672
CGGTCTA3750.039.30666731
TAGGGTA7850.039.235675
TCACGAC3950.038.98734325
ACGTAAG404.1261592E-738.51
TTCGAAC404.1261592E-738.539
CTCACGA3850.038.28571324
CGTAAGG1900.038.210532
GTTGATC6600.038.016
Produced by FastQC (version 0.11.5)