##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546080_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1735487
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.765183490282556	31.0	28.0	31.0	16.0	33.0
2	28.690426375997056	31.0	28.0	33.0	16.0	33.0
3	28.159951068489708	31.0	27.0	33.0	16.0	34.0
4	32.15124112136824	35.0	32.0	35.0	25.0	37.0
5	33.64897691541337	35.0	33.0	35.0	28.0	37.0
6	33.528438991476165	35.0	33.0	37.0	28.0	37.0
7	34.291858711704556	35.0	35.0	37.0	31.0	37.0
8	34.59402980258567	35.0	35.0	37.0	32.0	37.0
9	36.366248782042156	39.0	35.0	39.0	31.0	39.0
10	36.07202531623688	37.0	35.0	39.0	31.0	39.0
11	36.333444157173176	38.0	35.0	39.0	32.0	39.0
12	35.02552712869644	37.0	34.0	39.0	28.0	39.0
13	35.40257288011953	37.0	35.0	39.0	30.0	39.0
14	36.049786025478724	38.0	34.0	40.0	29.0	41.0
15	36.6314705900995	38.0	35.0	40.0	31.0	41.0
16	36.2267202231996	38.0	34.0	40.0	31.0	41.0
17	35.81443018587866	37.0	34.0	40.0	30.0	41.0
18	35.4820191681067	37.0	34.0	39.0	29.0	40.0
19	34.99830537480258	37.0	33.0	39.0	27.0	41.0
20	35.23053586687771	36.0	34.0	39.0	29.0	40.0
21	35.39163128274657	36.0	34.0	39.0	30.0	40.0
22	35.80859263134786	37.0	34.0	39.0	31.0	40.0
23	36.18963783652658	37.0	35.0	40.0	31.0	41.0
24	35.739285860395384	36.0	34.0	40.0	31.0	41.0
25	35.424797765699196	36.0	34.0	40.0	30.0	41.0
26	35.574596640597136	36.0	34.0	40.0	30.0	41.0
27	35.919446241890604	37.0	35.0	40.0	31.0	41.0
28	35.80739412049759	36.0	35.0	40.0	31.0	41.0
29	35.95317625542571	37.0	35.0	40.0	31.0	41.0
30	35.60372621633005	36.0	35.0	40.0	30.0	41.0
31	34.87881441923794	36.0	34.0	40.0	28.0	41.0
32	34.88324026627684	36.0	34.0	40.0	27.0	41.0
33	34.550125123380354	36.0	34.0	40.0	24.0	41.0
34	34.11649294981754	36.0	34.0	40.0	21.0	41.0
35	33.87652111482252	37.0	33.0	40.0	20.0	41.0
36	33.45900660736727	36.0	33.0	40.0	18.0	41.0
37	33.38541458391794	36.0	33.0	40.0	18.0	41.0
38	33.26344017558184	36.0	33.0	40.0	17.0	41.0
39	33.05164947936804	36.0	33.0	40.0	15.0	41.0
40	32.82873337570376	36.0	32.0	40.0	15.0	40.0
41	32.52790427125066	35.0	32.0	39.0	15.0	40.0
42	32.70955357199449	35.0	32.0	40.0	15.0	40.0
43	32.493168776257036	35.0	32.0	39.0	14.0	40.0
44	32.228849308580244	35.0	31.0	39.0	10.0	40.0
45	32.19428609952134	35.0	31.0	39.0	12.0	40.0
46	32.016749189132504	35.0	31.0	39.0	11.0	40.0
47	31.99297776359028	35.0	31.0	39.0	11.0	40.0
48	32.0098831048576	35.0	31.0	39.0	11.0	40.0
49	32.05643660828344	35.0	31.0	39.0	11.0	40.0
50	32.21000099683835	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	7.0
13	28.0
14	70.0
15	194.0
16	514.0
17	1063.0
18	1974.0
19	3472.0
20	5327.0
21	8075.0
22	11261.0
23	15803.0
24	23497.0
25	34783.0
26	49319.0
27	55764.0
28	53622.0
29	52127.0
30	57330.0
31	68380.0
32	88989.0
33	131069.0
34	201877.0
35	160929.0
36	172076.0
37	238169.0
38	251333.0
39	48427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.76840909784977	21.867464291003046	26.02623932072093	23.33788729042626
2	39.2387842720804	19.523453647304763	28.281110719930485	12.956651360684349
3	28.172034708413257	18.285472607976896	40.11185332992987	13.430639353679977
4	24.840981234662085	24.60583110101084	35.9068664876199	14.646321176707172
5	20.12132617530411	27.966674483876858	37.47466849362744	14.437330847191596
6	18.663176387953353	35.174622454676985	35.54149354042986	10.620707616939798
7	72.70552876512471	5.772904089745414	18.181928184999368	3.3396389601305
8	73.50063699699277	4.270443973363096	18.266227289515854	3.9626917401282755
9	69.57240244380972	5.985005937814574	19.87246231173152	4.570129306644187
10	40.364750643479326	21.142365226590577	25.350578828882036	13.142305301048063
11	35.289575779017646	21.15256409296065	29.65830340417416	13.899556723847542
12	32.223635210174436	19.567418252052594	33.73243360509183	14.476512932681143
13	20.733661502506212	29.895470262813838	34.46462001732078	14.906248217359162
14	16.269900033823358	32.36757175363457	36.086585494446226	15.275942718095841
15	15.666726400140133	21.78183991006559	47.67589731297325	14.87553637682103
16	17.06990602637761	18.752718977439763	46.5242321031503	17.65314289303233
17	17.44478639137026	19.189656851362184	36.124730407084584	27.240826350182974
18	20.761722790202406	21.567721337008	39.1879628023719	18.4825930704177
19	24.335244228277134	23.297034204232013	34.36044176648975	18.007279801001104
20	25.487139920955904	22.079450897644293	33.24703671073307	19.186372470666736
21	21.42516769068279	24.503496713026372	35.85794650147192	18.21338909481892
22	19.88127828096667	21.80638633421051	34.684558282487856	23.627777102334964
23	18.650845555166935	26.2738355285865	34.03989773475688	21.035421181489692
24	19.217199552632778	22.270002598694198	41.215347622886256	17.29745022578677
25	18.65695335084619	21.289470909318247	39.18778994023003	20.86578579960553
26	16.76370955241958	26.491526585909313	35.4535643309342	21.291199530736908
27	17.325223409913182	26.961711611783894	36.78535189258116	18.92771308572176
28	15.474618939813437	25.192409969074962	40.84415498358674	18.488816107524862
29	16.72879139976272	23.864771098832776	39.09260052077601	20.31383698062849
30	18.786657577959385	24.682927616282925	37.81578312024233	18.71463168551536
31	24.4402867898175	22.950042264793684	33.57766436740811	19.032006577980706
32	25.13928367080825	23.12399920022449	33.718201288744886	18.01851584022237
33	23.73270442244742	24.074856221913503	32.28770944409264	19.904729911546443
34	18.42232180362054	25.17702523844892	33.70460280025146	22.696050157679085
35	18.864733645368705	24.366705138096684	34.3947260912931	22.373835125241502
36	25.311569605534356	24.309603010567063	30.862691567266133	19.51613581663245
37	19.322299734887093	28.772673030682455	31.41077979840817	20.49424743602228
38	19.893032906613534	29.548881668373202	29.17578754551316	21.382297879500108
39	19.815129701346077	26.355599321688956	30.6635543798369	23.165716597128068
40	22.030588532210267	23.042811614261588	32.16249963266795	22.764100220860197
41	17.833841451995895	23.202709095487318	31.97304272518319	26.990406727333593
42	20.942248487024102	24.83959833752716	29.022458825678328	25.19569434977041
43	20.470104356875044	24.504591506591524	30.323649788215068	24.70165434831837
44	19.594903332609235	26.6404185107696	30.872314226496655	22.892363930124514
45	19.136530553095472	29.914427477705107	28.31683556258272	22.6322064066167
46	21.255417067370715	27.240711108755068	30.251739137198953	21.252132686675267
47	20.483069017514968	25.093244720358033	31.0853380059891	23.338348256137902
48	20.389262495195872	24.741700744517246	32.58526281095738	22.283773949329497
49	20.564487086333692	23.555866451318852	33.27089168631053	22.60875477603693
50	19.732501597534295	26.45194115542208	31.54659182120062	22.268965425843003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	939.0
1	1659.5
2	2380.0
3	29452.5
4	56525.0
5	38964.0
6	21403.0
7	21075.5
8	20748.0
9	21646.0
10	22544.0
11	22122.5
12	21701.0
13	20798.0
14	19895.0
15	18404.0
16	16913.0
17	15975.5
18	15038.0
19	13871.5
20	12705.0
21	12174.5
22	11644.0
23	11275.0
24	10906.0
25	11191.0
26	11476.0
27	14189.0
28	16902.0
29	17313.0
30	17724.0
31	20074.0
32	22424.0
33	26527.5
34	30631.0
35	35048.0
36	39465.0
37	45140.0
38	50815.0
39	59326.0
40	67837.0
41	90909.5
42	113982.0
43	129106.5
44	144231.0
45	146610.0
46	148989.0
47	148499.5
48	148010.0
49	149389.0
50	150768.0
51	136764.5
52	122761.0
53	106888.5
54	91016.0
55	82650.0
56	74284.0
57	72472.5
58	70661.0
59	64547.0
60	58433.0
61	49788.0
62	41143.0
63	35534.0
64	29925.0
65	23962.0
66	17999.0
67	14872.0
68	11745.0
69	9877.0
70	8009.0
71	6681.0
72	5353.0
73	4451.0
74	3549.0
75	2748.5
76	1948.0
77	1454.5
78	961.0
79	755.0
80	549.0
81	408.0
82	267.0
83	186.0
84	105.0
85	95.0
86	85.0
87	61.5
88	38.0
89	27.0
90	16.0
91	13.0
92	10.0
93	9.5
94	9.0
95	17.5
96	26.0
97	13.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1735487.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.69539654746372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21774034090522	20.880535954470144
2	7.180102399288536	3.8335136160080636
3	2.7373976642694426	2.1922774846732116
4	1.5817734676229624	1.6890427986580698
5	1.124158195234213	1.500492440192923
6	0.8626443074977723	1.3817179120839156
7	0.6873190016244062	1.2843777312079268
8	0.5800047955821126	1.2386766413996104
9	0.47812461395818656	1.1487353551845114
>10	5.240329374237394	30.53084950993802
>50	0.9580384283770363	17.92406941779059
>100	0.34665927948080044	13.00318605206114
>500	0.004610414244632639	0.9165121758669552
>1k	8.781741418347882E-4	0.31917926267882324
>5k	0.0	0.0
>10k+	2.1954353545869704E-4	2.1568336477861503
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36801	2.12049989426599	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1801	0.10377490583334822	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.762071395521833E-5	0.0	0.0	0.08043851668148479	0.0
2	5.762071395521833E-5	0.0	0.0	0.29778384972056837	0.0
3	5.762071395521833E-5	0.0	0.0	0.3913022684698877	0.0
4	5.762071395521833E-5	0.0	0.0	0.5569042003771852	0.0
5	5.762071395521833E-5	0.0	0.0	0.9382380853328202	0.0
6	5.762071395521833E-5	0.0	0.0	1.3074716203578591	0.0
7	1.1524142791043666E-4	0.0	0.0	1.5683205924331327	0.0
8	1.1524142791043666E-4	0.0	0.0	2.211828725885011	0.0
9	1.1524142791043666E-4	0.0	0.0	2.453547620927152	0.0
10	1.1524142791043666E-4	0.0	0.0	2.8525710650670386	0.0
11	1.1524142791043666E-4	0.0	0.0	3.2216893586641673	0.0
12	1.1524142791043666E-4	0.0	0.0	3.5231033133639147	0.0
13	2.3048285582087332E-4	0.0	0.0	3.650675574060768	0.0
14	2.8810356977609165E-4	0.0	0.0	3.7004598709180767	0.0
15	4.033449976865283E-4	0.0	0.0	3.7717366940806816	0.0
16	4.6096571164174665E-4	0.0	0.0	3.938952005978725	0.0
17	4.6096571164174665E-4	0.0	0.0	4.135496261279975	0.0
18	4.6096571164174665E-4	0.0	0.0	4.446360013068378	0.0
19	5.762071395521833E-4	0.0	0.0	4.567421133088292	0.0
20	5.762071395521833E-4	0.0	0.0	4.7034636387365625	0.0
21	5.762071395521833E-4	0.0	0.0	4.87701722916968	0.0
22	5.762071395521833E-4	0.0	0.0	5.055295718147125	0.0
23	5.762071395521833E-4	0.0	0.0	5.2476912820436	0.0
24	5.762071395521833E-4	0.0	0.0	5.394163136917764	0.0
25	6.9144856746262E-4	0.0	0.0	5.514993774081857	0.0
26	6.9144856746262E-4	0.0	0.0	5.634729617680801	0.0
27	7.490692814178384E-4	0.0	0.0	5.769389226194146	0.0
28	8.64310709328275E-4	0.0	0.0	5.907217973975028	0.0
29	9.795521372387116E-4	0.0	0.0	6.057031830258596	0.0
30	9.795521372387116E-4	0.0	0.0	6.238594699931489	0.0
31	9.795521372387116E-4	0.0	0.0	6.404023769696921	0.0
32	0.00103717285119393	0.0	0.0	6.546462174594221	0.0
33	0.00103717285119393	0.0	0.0	6.70082806728025	0.0
34	0.0010947935651491483	0.0	0.0	6.854675373540683	0.0
35	0.0010947935651491483	0.0	0.0	7.037851623204323	0.0
36	0.0010947935651491483	0.0	0.0	7.200918243697591	0.0
37	0.0010947935651491483	0.0	0.0	7.354362204960337	0.0
38	0.0010947935651491483	0.0	0.0	7.538345144619349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.857593E-4	44.0	19
GTCGATG	60	0.0	44.0	1
CGCATCG	30	2.5284662E-6	44.0	21
CCCGTAT	30	2.5284662E-6	44.0	34
TCTACCG	20	7.857593E-4	44.0	1
TCTATCG	20	7.857593E-4	44.0	17
CGACGGT	350	0.0	43.37143	28
CGTTTTT	22235	0.0	43.21835	1
CGGGCTA	145	0.0	40.96552	6
GACGGTC	375	0.0	40.48	29
TATAGCG	155	0.0	39.741932	1
GTTTTTT	25025	0.0	39.331867	2
CGGTCTA	375	0.0	39.306667	31
TAGGGTA	785	0.0	39.23567	5
TCACGAC	395	0.0	38.987343	25
ACGTAAG	40	4.1261592E-7	38.5	1
TTCGAAC	40	4.1261592E-7	38.5	39
CTCACGA	385	0.0	38.285713	24
CGTAAGG	190	0.0	38.21053	2
GTTGATC	660	0.0	38.0	16
>>END_MODULE