FastQCFastQC Report
Thu 26 May 2016
SRR1546079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546079_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1707668
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366232.144620617122298No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18840.110325894728952No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC207.8575575E-444.030
TATACGT207.8575575E-444.016
CGTTTTT213400.043.1958771
CGACGGT3150.042.60317628
CGGTCTA3300.042.031
TCACGAC3200.041.937525
ACGTAAG557.8216544E-1140.01
TAGCCGT452.3521352E-839.1111144
GCGATAC452.3521352E-839.111119
TAGGGCG5800.039.0689665
GTTTTTT241400.039.0240252
GGGCGAT17100.038.9824567
GACGGTC3450.038.8985529
ACGGTCT3450.038.8985530
TATTACG404.1261046E-738.51
GTCGATG750.038.133331
ATTCGGC1100.038.017
TAGGGTA7700.038.05
AGGGCGA9500.037.978956
CACGGGA3200.037.81254
Produced by FastQC (version 0.11.5)