FastQCFastQC Report
Thu 26 May 2016
SRR1546078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546078_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1428971
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196311.3737857521251304No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT254.443385E-544.034
CGTTTTT195050.043.4360431
CGGTCTA2650.040.67924531
GCGGAAT5050.040.514858
TGCGACG603.6379788E-1240.3333321
CGACGGT2700.039.9259328
TAACGAC501.3478711E-939.60000225
TCACGAC2450.039.51020425
CGAAGGC950.039.3684233
CGGAATT4700.039.3191459
ATAGGCG1850.039.243244
GTTTTTT222800.038.7567332
CTCACGA2450.038.61224424
TAGGGTA6900.037.9420285
CGTTAGG2450.037.7142872
ATTCGGC1000.037.417
CCGTTGG1650.037.3333322
TATCGGG2600.037.2307663
GGCGGGA21100.037.2227487
TAGGCGA1800.036.6666685
Produced by FastQC (version 0.11.5)