##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546078_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1428971
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.56606117269	28.0	16.0	30.0	16.0	33.0
2	26.01874075820993	30.0	25.0	31.0	10.0	33.0
3	26.350576043880526	30.0	25.0	31.0	16.0	33.0
4	27.965630513145474	32.0	19.0	35.0	10.0	35.0
5	31.130671651139178	33.0	30.0	35.0	19.0	37.0
6	29.800903587266642	33.0	28.0	35.0	17.0	36.0
7	29.777439150269668	32.0	28.0	35.0	17.0	35.0
8	32.31837245122539	33.0	32.0	35.0	28.0	35.0
9	27.02149588760024	30.0	23.0	34.0	10.0	35.0
10	31.05231036878985	34.0	27.0	37.0	17.0	37.0
11	33.51059118764481	35.0	32.0	37.0	27.0	39.0
12	33.03766836415854	35.0	31.0	37.0	23.0	39.0
13	33.28403865438837	35.0	32.0	38.0	24.0	39.0
14	32.980663008556505	35.0	31.0	39.0	21.0	40.0
15	31.809662337444216	34.0	27.0	38.0	18.0	40.0
16	30.385404602332727	33.0	26.0	38.0	17.0	40.0
17	30.57072396850601	33.0	26.0	38.0	17.0	40.0
18	31.503867468269124	34.0	27.0	38.0	17.0	39.0
19	31.270172032882403	34.0	27.0	37.0	18.0	39.0
20	31.784377009750372	34.0	29.0	37.0	19.0	39.0
21	32.66148018399254	34.0	31.0	37.0	23.0	39.0
22	33.0400295037478	35.0	31.0	37.0	25.0	39.0
23	34.30343302978157	35.0	33.0	38.0	28.0	40.0
24	33.91374492554433	35.0	33.0	38.0	26.0	40.0
25	32.773415975551636	35.0	31.0	38.0	23.0	39.0
26	26.349899333156515	33.0	19.0	36.0	0.0	39.0
27	29.65124414701208	34.0	23.0	37.0	15.0	39.0
28	31.094541456754545	34.0	27.0	37.0	18.0	39.0
29	32.397974486536114	34.0	30.0	37.0	23.0	39.0
30	32.188817687692755	34.0	30.0	37.0	22.0	39.0
31	30.98966179159689	34.0	29.0	36.0	18.0	39.0
32	31.325205340066383	34.0	30.0	37.0	18.0	39.0
33	30.971468280321993	34.0	29.0	37.0	17.0	39.0
34	30.881684092959198	34.0	29.0	37.0	15.0	39.0
35	30.075284242997235	34.0	27.0	37.0	10.0	39.0
36	29.839065313431835	34.0	26.0	37.0	10.0	39.0
37	29.953831113437573	34.0	27.0	37.0	10.0	39.0
38	29.68361919171208	33.0	26.0	37.0	10.0	39.0
39	29.645144653040546	34.0	26.0	37.0	10.0	39.0
40	29.505170503810085	33.0	26.0	37.0	10.0	39.0
41	28.898654346379317	33.0	24.0	36.0	9.0	38.0
42	29.070936359100358	33.0	25.0	36.0	9.0	38.0
43	28.611923544984467	33.0	24.0	36.0	9.0	38.0
44	28.391173088887037	32.0	23.0	36.0	8.0	38.0
45	27.857725594151315	32.0	23.0	35.0	8.0	38.0
46	27.46851195720557	31.0	23.0	35.0	8.0	38.0
47	27.528488681715725	31.0	23.0	35.0	8.0	38.0
48	27.589458428477556	31.0	23.0	35.0	8.0	38.0
49	27.42534453113464	31.0	23.0	35.0	8.0	38.0
50	29.219665059682807	33.0	24.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	8.0
11	34.0
12	87.0
13	226.0
14	519.0
15	1158.0
16	2327.0
17	4258.0
18	7525.0
19	11335.0
20	16540.0
21	22894.0
22	30397.0
23	39575.0
24	50719.0
25	63035.0
26	72238.0
27	79513.0
28	86902.0
29	100351.0
30	118295.0
31	137068.0
32	149987.0
33	148572.0
34	131096.0
35	97305.0
36	47246.0
37	9511.0
38	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.903205173512966	22.26490250676886	25.70283091819218	25.12906140152599
2	39.908297649147535	16.550860724255426	29.760436006049108	13.780405620547933
3	29.556233121595888	13.899652267260848	42.388963806823234	14.155150804320032
4	26.103608820612873	24.222255035266635	35.4231121555301	14.251023988590392
5	19.162600220718264	28.005116968783835	38.87454678926304	13.95773602123486
6	18.31072848924156	33.61670740693828	37.429590943413125	10.642973160407033
7	75.85255404063483	3.7359050673526615	18.381198778701595	2.030342113310907
8	77.39317312947568	2.2154403413365285	18.14571464361418	2.2456718855736053
9	64.90341651440093	3.502170442927113	18.23563949163419	13.358773551037775
10	41.717851516930715	20.72435339835448	25.789116784035503	11.7686783006793
11	36.4161344072063	20.47634276692809	30.26933366737324	12.83818915849237
12	32.91886259413242	19.46484568266256	34.72407767547417	12.89221404773085
13	19.926366595263307	30.67752949500025	35.249280776166906	14.14682313356954
14	15.381067915304088	32.61304813043792	37.343724960128654	14.662158994129342
15	13.940730777601503	19.8353220604197	48.77873658737651	17.445210574602285
16	15.130747929804034	16.394454471084437	47.54533157075966	20.92946602835187
17	16.40635114358514	17.771319361974456	37.5957244758641	28.226605018576308
18	20.088861145537592	20.786635977916976	40.98886541434361	18.135637462201824
19	24.7894463918442	22.24775730228255	35.79533804394911	17.16745826192414
20	25.94398346782405	21.305330898947563	34.444715812987106	18.305969820241277
21	21.261733093253817	23.659612406409927	37.21384128859158	17.864813211744675
22	19.51278227479774	21.151024058570815	35.56608216681794	23.770111499813503
23	18.045712614181813	25.919000455572576	35.1518680225141	20.883418907731507
24	18.300231425270354	21.320656612345527	43.92230493131071	16.45680703107341
25	18.013101735444597	19.627760115495697	41.71708173223949	20.642056416820214
26	13.197258726734132	38.56600308893602	30.087174617259553	18.149563567070288
27	16.18479311336619	27.545485527697906	38.253610465152896	18.016110893783008
28	14.54046303248981	24.84123190743549	43.008850424536256	17.60945463553844
29	15.772188518871271	23.311109882565848	41.03162345491966	19.885078143643224
30	17.983150112913417	24.353748256612626	39.32794997239272	18.335151658081234
31	24.973844815605077	22.261753387577496	34.771174502491654	17.993227294325777
32	25.371193677128506	22.37141271586337	34.97971617338631	17.277677433621815
33	23.927287537675713	23.478363101840415	33.19087651183964	19.403472848644235
34	18.10533593753827	24.79224560890319	34.074099474377014	23.028318979181524
35	18.573924873212963	23.923648555499028	34.95326357217886	22.54916299910915
36	25.76392383050461	23.60411792821548	31.014275307196577	19.617682934083337
37	18.72228337733936	29.211439560354968	31.38104272235056	20.685234339955112
38	19.290384479461096	30.119225652584973	29.076237376405818	21.514152491548113
39	19.35637602162675	26.440564574088626	30.794956650624822	23.4081027536598
40	21.81464844283054	22.421098818660422	32.49953987869593	23.264712859813113
41	17.049541243314245	22.53460707040241	32.36398779261441	28.051863893668944
42	20.35212750993547	24.172219030337214	28.570348873420105	26.90530458630721
43	20.052681265050165	23.75079690210648	30.622454899364648	25.574066933478708
44	18.842719691302342	26.19766251379489	31.310642413316998	23.64897538158577
45	18.480780925575115	31.058922819287442	27.787757764153366	22.67253849098407
46	20.569766636271837	27.017273268666752	30.054773679801762	22.358186415259652
47	20.013772147930222	24.543255251506153	30.71091015842869	24.732062442134936
48	19.699280111352856	24.295314600506238	33.33447634696575	22.670928941175152
49	20.045403300696798	23.379690700511066	33.11935651598248	23.45554948280966
50	19.253084912150072	26.915801650278414	31.693995189545483	22.137118248026027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	478.0
1	991.0
2	1504.0
3	17618.0
4	33732.0
5	26470.5
6	19209.0
7	18721.0
8	18233.0
9	19350.5
10	20468.0
11	20899.5
12	21331.0
13	21067.0
14	20803.0
15	20005.5
16	19208.0
17	18275.0
18	17342.0
19	16215.0
20	15088.0
21	14361.0
22	13634.0
23	12953.5
24	12273.0
25	12336.5
26	12400.0
27	13934.0
28	15468.0
29	16091.0
30	16714.0
31	18358.5
32	20003.0
33	22885.0
34	25767.0
35	28200.5
36	30634.0
37	34798.0
38	38962.0
39	45128.5
40	51295.0
41	69368.5
42	87442.0
43	99860.5
44	112279.0
45	113950.5
46	115622.0
47	115660.5
48	115699.0
49	115537.0
50	115375.0
51	107165.5
52	98956.0
53	87504.0
54	76052.0
55	70051.5
56	64051.0
57	61582.5
58	59114.0
59	54374.0
60	49634.0
61	43129.0
62	36624.0
63	31554.0
64	26484.0
65	21736.0
66	16988.0
67	13925.5
68	10863.0
69	9061.0
70	7259.0
71	6076.0
72	4893.0
73	4090.0
74	3287.0
75	2565.5
76	1844.0
77	1383.5
78	923.0
79	716.0
80	509.0
81	359.5
82	210.0
83	166.5
84	123.0
85	102.5
86	82.0
87	60.0
88	38.0
89	27.0
90	16.0
91	16.5
92	17.0
93	17.5
94	18.0
95	18.0
96	18.0
97	11.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1428971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.41464683290392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5763384763863	35.024462332302605
2	7.078853744274774	6.004941630903881
3	2.674830996263387	3.403560361326483
4	1.4277631821549988	2.422322845285094
5	0.9195258285002872	1.9500681634786527
6	0.6581645218094636	1.6749489450297292
7	0.4979548225037777	1.4784404554667363
8	0.3845394611181945	1.3048084349314724
9	0.32191696463664493	1.2288594928125343
>10	2.9630239016097524	26.429958837739637
>50	0.409602662014075	11.801879670485453
>100	0.0856703886870609	5.31464041730141
>500	0.001633545037101239	0.44982163997752
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.815050041223599E-4	1.5112867729588733
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19631	1.3737857521251304	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06410207065083896	0.0
2	0.0	0.0	0.0	0.23422448741087118	0.0
3	0.0	0.0	0.0	0.309663387150614	0.0
4	0.0	0.0	0.0	0.4182730090393717	0.0
5	0.0	0.0	0.0	0.6841986296432888	0.0
6	0.0	0.0	0.0	0.9079960335094274	0.0
7	0.0	0.0	0.0	1.0700707012248674	0.0
8	0.0	0.0	0.0	1.4604915005273025	0.0
9	0.0	0.0	0.0	1.6447499634352272	0.0
10	0.0	0.0	0.0	1.9183034505248882	0.0
11	0.0	0.0	0.0	2.1702329858338625	0.0
12	0.0	0.0	0.0	2.3777249503313924	0.0
13	0.0	0.0	0.0	2.4631710510570195	0.0
14	0.0	0.0	0.0	2.492702791029349	0.0
15	0.0	0.0	0.0	2.539519696340933	0.0
16	0.0	0.0	0.0	2.6328735852582033	0.0
17	0.0	0.0	0.0	2.785011032414234	0.0
18	0.0	0.0	0.0	2.970809064704602	0.0
19	0.0	0.0	0.0	3.0374304307085307	0.0
20	0.0	0.0	0.0	3.1202172752281188	0.0
21	0.0	0.0	0.0	3.2165103420573264	0.0
22	0.0	0.0	0.0	3.3219008643282475	0.0
23	0.0	0.0	0.0	3.4340794879672156	0.0
24	0.0	0.0	0.0	3.519735529972267	0.0
25	0.0	0.0	0.0	3.5897859368734566	0.0
26	0.0	0.0	0.0	3.6645950127749267	0.0
27	0.0	0.0	0.0	3.761727844721831	0.0
28	0.0	0.0	0.0	3.8577409898451402	0.0
29	0.0	0.0	0.0	3.969219809219361	0.0
30	0.0	0.0	0.0	4.101272873977148	0.0
31	0.0	0.0	0.0	4.226397876513939	0.0
32	0.0	0.0	0.0	4.335707302667444	0.0
33	0.0	0.0	0.0	4.449915358674179	0.0
34	0.0	0.0	0.0	4.566712690460478	0.0
35	0.0	0.0	0.0	4.707093425968757	0.0
36	6.998042647471502E-5	0.0	0.0	4.826270092255196	0.0
37	6.998042647471502E-5	0.0	0.0	4.946776386644656	0.0
38	6.998042647471502E-5	0.0	0.0	5.07966921652014	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	25	4.443385E-5	44.0	34
CGTTTTT	19505	0.0	43.436043	1
CGGTCTA	265	0.0	40.679245	31
GCGGAAT	505	0.0	40.51485	8
TGCGACG	60	3.6379788E-12	40.333332	1
CGACGGT	270	0.0	39.92593	28
TAACGAC	50	1.3478711E-9	39.600002	25
TCACGAC	245	0.0	39.510204	25
CGAAGGC	95	0.0	39.368423	3
CGGAATT	470	0.0	39.319145	9
ATAGGCG	185	0.0	39.24324	4
GTTTTTT	22280	0.0	38.756733	2
CTCACGA	245	0.0	38.612244	24
TAGGGTA	690	0.0	37.942028	5
CGTTAGG	245	0.0	37.714287	2
ATTCGGC	100	0.0	37.4	17
CCGTTGG	165	0.0	37.333332	2
TATCGGG	260	0.0	37.230766	3
GGCGGGA	2110	0.0	37.222748	7
TAGGCGA	180	0.0	36.666668	5
>>END_MODULE